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Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

This interview was transcribed from video as the interviewee expressed a wish to remain anonymous ('Henry Deacon' is a pseudonym, prompted by his similarity to the likeable and creative polymath on the Eureka TV series).

此次采访是按照被采访者的意愿保持匿名从采访录像上转录的（Henry Deacon是假名，是从ERUEKA系列电视节目中可爱的有创造性的学者得到类似的提示

The radiologist's dictation is transcribed in a single document, which covers all three orders.

放射线治疗师在一个单一的覆盖所有三张单子的档案中的口述被转录。

Methods Entire coding sequence of CSP was reverse transcribed and amplified by RT-PCR with sporozoite total RNA as template,then cloned into pFLAG-CMV8 for construction of the recombinant plasmid pFLAG-CMV8-CSP.

以约氏疟原虫BY265株子孢子总RNA为模板，用RT-PCR扩增CSP基因的编码区序列并克隆至pFLAG-CMV8载体，构建重组质粒pFLAG-CMV8-CSP。

Du Daozheng, once the head of China's state publishing watchdog, says he and the three other former officials transcribed recordings made by Zhao that were later turned into the recently published English-language book "Prisoner of the State."

曾经担任中国国家出版监督机构新闻出版署署长的杜导正说，他和其他 3 位中共前官员将赵紫阳的录音转為文字，后来这些文字被翻译成了最近出版的英文书《国家的囚徒》。

The account of the trial was transcribed from the stenographer 's shorthand notes.

这篇审判的报道是从速记员的笔记中抄录而来的。

The account of the trial was transcribed from the stenographer's shorthand notes.

注：审讯的报告是从速记员的速记本上转录过来的。

Stevensonian scholar Barry Menikoff offers a challenging new approach to the old favourite in this handsome edition transcribed from the original manuscript in the Huntington Library (and, for the final chapters, from the 1886 serialised version).

巴里stevensonian学者提供一个具有挑战性的新途径menikoff旧喜爱这个英俊版的转录原稿的亨廷顿图书馆(并为最终章，从1886serialised版)。

The internal transcribed spacer regions of nuclear ribosomal DNA from 8 species of subgenus Amygdalus were sequenced, and analyzed together with other ITS data of 18 species representing subgenus Cerasus, subgenus Armeniaca and subgenus Primus by using of Padus racemosa Gilib. and Padus buergeriana Yüet Ku as outgroup for studying phylogeny of subgenera in stone fruit plants.

采用核核糖体DNA内转录间隔区对桃亚属的光核桃、甘肃桃、新疆桃、山桃、陕甘山桃、扁桃、野扁桃基因序列测序及来源于GenBank的李、杏、梅、樱的18个种的ITS区基因序列，以稠李和楼木、梅和杏分别作为对桃亚属及其近缘亚属植物、桃亚属各种类的外类群，进行系统发育树的建立。

Superpure DNA can be prepared using RNA transcribed from DNA in the course of gene expression.

在基因表达期间，使用从DNA 转录而来的RNA可制备出超纯度的DNA。

Pradeep Indulkar, a senior official at the Maharashtra state Home Ministry say 101 human were killed and 287 injured.

马哈拉施特拉邦内政部高级官员Pradeep Indulkar称，有101人在冲突中丧生，287人受伤。

Mark Frei, a senior vice president of West, says 80% of West's homeagents have some college education, compared with 30% of those whowork in office-based call centers.

Frei说，该公司80%在家办公的人员都拥有本科学位，而在公司电话中心工作的人中只有30%拥有本科学位。