查询词典 toxin

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

At the same time, we separate and purify the Actinobacillus pleuropneumoniae-RTX-toxin by the seperation and purificationtechnique ofprotein and get pure apxl and apx II. Then, we research their biological characterization primarily.

同时我们还利用蛋白质层析技术对APP分泌的天然溶血毒素(Actinobacillus pleuropneumoniae-RTX-toxin，APX)进行了分离纯化，并获得了高纯度的溶血毒素Ⅰ，并对其生物学活性进行了初步的研究。

The book comprehensively reviews the molecular and cellular biology of the most important microbial toxins with a focus on recent advances in the field, covering topics such as toxin ADP-ribosylation of proteins, toxin inhibition of protein synthesis, modulation of actin cytoskeleton, cytolethal distending toxins, cell-damaging/pore-forming toxins, clostridial neurotoxins, toxins of the immune system, streptococcal and staphylococcal toxins, anthrax toxins, Shiga toxin, Yersinia toxin, mycotoxins, and the regulation of toxin expression, as well as the potential of microbial toxins in bioterrorism.

该书全面评论了最重要的微生物毒素的分子与细胞生物学，重点是这一领域中的最新进展，主题包括蛋白质毒素ADP核糖基化，毒素抑制蛋白质合成，肌动蛋白细胞骨架的调控，细胞致死增加毒素，细胞损伤/孔状毒素，梭菌属的神经毒素，免疫系统的毒素，链球菌和葡萄球菌毒素，炭疽毒素，Shiga毒素，Yersinia毒素，毒枝菌素，和毒素表达调节，以及生物恐怖中微生物毒素的潜在可能性。

Therefore, in the present study, a retrograde and anterograde experiments were made in the rat to address this issue. After injection of cholera toxin B subunit, a retrograde tracer, into Barrington's nucleus or D-region, some retrogradely labeled neurons were found in lamina I, sacral parasympathetic nucleus and dorsal commissural nucleus of the lumbosacral spinal cord segments. Injection of biotinylated dextran amine into lamina I, sacral parasympathetic nucleus and dorsal commissural nucleus, respectively, a large number of BDA-labeled axon terminals were seen in Barrington's nucleus and D-region.

发现，将逆行示踪剂霍乱毒素 B 亚单位（ Cholera toxin B subunit； CTb ）注射到 Barrington's 核或 D 区后，在大鼠腰骶髓 I 层、骶髓副交感核和后连合核内分布有一定数量的 CTb 逆标神经元；将顺行示踪剂结合生物素的葡聚糖胺(Biotinylated dextran amine； BDA)分别注射到腰骶髓 I 层、骶髓副交感核和后连合核内后， Barrington's 核和 D 区内出现大量顺行标记的轴突终末。

If the urethral resistance can be reduced by botulinum A toxin injection, patients with detrusor underactivity might restore voiding efficacy by abdominal straining or the Crede maneuver, and even discontinue intermittent catheterization or remove their cystostomy.

如果可以利用注射botulinum A toxin使得尿道阻力减少，那麼逼尿肌膀胱收缩不良病患就可以利用腹部用力，或在下腹部两旁搓压迫膀胱促进排空尿液，甚至以间歇性导尿，恢复排尿能力。

If the urethral resistance can be reduced by botulinum A toxin injection, patients with detrusor underactivity might restore voiding efficacy by abdominal straining or the Crede maneuver, and even discontinue intermittent catheterization or remove their cystostomy.

如果可以利用注射botulinum A toxin使得尿道阻力减少，那么逼尿肌膀胱收缩不良病患就可以利用腹部用力，或在下腹部两旁搓压迫膀胱促进排空尿液，甚至以间歇性导尿，恢复排尿能力。

Edema disease is a common cause of illness and death loss in pigs during the first 2 weeks after weaning. The disease is an enterotoxemia caused by Shiga-like toxin Escherichia coli that colonize the small intestine and produce SLT-IIe.

猪水肿病(Porcine Edema disease，ED)是仔猪常见的一种急性致死性传染病，主要由某些特定血清型产类志贺毒素大肠杆菌(Shiga-like toxin Escherichia coli，SLTEC)引起的肠毒血症。

This study was aimed to construct and characterize Salmonella vaccine strain C500 expressing the recombinant Pasteurella multocida toxin antigen by the Asd+ balanced-lethal host-vector system.

本研究利用Asd+平衡致死系统构建表达巴氏杆菌毒素(Pasteurella multocida toxin， PMT)的重组猪霍乱沙门氏菌株，并对重组菌株的生物学特性进行比较研究。

The authors treated 20 patients who had voiding dysfunction with urethral injection of 50 U of botulinum A toxin.

研究中共治疗二十名排尿失能的病患，为他们进行剂量50 U的botulinum A toxin尿道注射。

The toxin-antitoxin system consists of a pair of co-expressed genes. The upstream gene encodes an unstable antitoxin protein, and the downstream gene encodes a stable toxin.

毒素-抗毒素系统(toxin-antitoxin system， TA)由两个共表达的基因组成，其中一个基因编码不稳定的抗毒素蛋白，另一个基因编码稳定的毒素蛋白。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。