查询词典 total cleavage

Recombinant hK5 with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.

经肠激酶切割和纯化，获得了与天然蛋白N-末端相同的hK5重组蛋白。

In addition, the r8 cDNA was also expressed at high level in pBAD/TOPO system and showed esterase activity after enterokinase cleavage followed by purification and renaturation.

此外类凝血酶基因r8在pBAD／TOPO中也得到高效融合表达，表达产物经酶切、纯化和复性，测得精氨酸酯酶活性。

The expressed IL-29 fusion protein was purified by Ni-NTA affinity chromatography and the fusion tag was removed from IL-29 fusion protein by cleavage with enterokinase.

纯化后的融合蛋白经肠激酶切割和回收后，所得目的蛋白（IL-29）纯度大于96％，该蛋白N-端序列与理论值一致，其抗病毒活性与IFN-α2b相当。

The fused insecticidal protein may delay the process of insects?developing resistance. After investigation of the secondary structure and action mode of Bt insecticidal protein and Cowpea trypsin proteinase inhibitor, as well as the action mechanism of insect enterokinase, we devised the fusion gene on the base of maintaining their functions respectively. The method follows: CpTI gene is linked to 3?-end of GFM crylA gene by a nucleotide sequence encoding cleavage site of insect enterokinase.

根据Bt杀虫蛋白Cry1A和豇豆胰蛋白酶抑制剂的高级结构、杀虫机理及昆虫体内肠激酶的作用机理，本着尽量不影响二者功能的原则，设计出了构建Cry1A和CpTI融合杀虫基因CryCI的方案，即将CpTI基因的编码序列连接到GFM Cry1A基因编码区3'端，并在其间用一段编码肠激酶切割位点的序列相连。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

The expressed protein was enriched with TALON resin and the SLC was released with enterokinase cleavage,purified with weak cation exchange chromatography.

用羊抗人SLC多克隆抗体进行Western blot分析显示，在30 kDa 处出现单一阳性条带，而对照的pET32a/BL21trxB(DE3)诱导菌则无此反应。3。

Based on the above experimental results, the DNA cleavage mechanisms of Ser-His and ethanolamine were proposed.

它们与丝组二肽一样，都可以在没有金属离子存在的条件下以水解方式切割DNA，这在世界上属首次报道。

This the god of fire did, and to the wonder of all the Olympians, a goddess, full-grown and full-armed, issued from the cleavage, radiating light and splendor

只见一位体态丰满、披坚执锐的女神从裂口处走了出来，光彩照人，仪态万方。

Computer analysis of the cleavage reaction of hammerhead ribozyme ［J］.

核酶对靶RNA的体外切割反应的计算机分析［J］。

DMBQ, ferulic acid and resorcin were effective chemical signal substances in the process of seed germination and haustorium formation of Cistanche deserticola. 4.The embryo is consist of 12 cells, the endosperm cell degraded so as to provide nutrition for the embryo development .As a result, germ tube differentiated from the embryo and came out through micropylar end, the small cell at the tip of the germ tube held the capacity of cleavage and the other cells grew big ,so the germ tube elongated .The anterior extremity of germ tube swelled to form haustorium under chemical substances treatment, the haustorium cell stained ,especially its portrait because of the lignification of the cells.

肉苁蓉种子的胚是由12个细胞紧密排列形成的圆球状胚，球形原胚分裂增殖的同时，胚乳细胞分解，为胚细胞的增殖提供营养，最终胚上分化出的芽管由珠孔端冒出，芽管前端细胞较小，具有旺盛的分裂能力，后部细胞体积增大，芽管逐渐伸长，在化学物质的诱导下，芽管由中部至前端细胞膨大分化出初生吸器，初生吸器呈帽子状，而这些细胞比其周围的细胞着色深，吸器纵向局部区域的番红染色加深，细胞高度木质化。

This paper designs the communication system of radio telescope FAST.

针对500m口径球面射电望远镜FAST分布式控制系统的特点完成了其通信系统的设计。

Political sheep's step-by-step a large number of fossils unearthed in the county soil Ma Ali after rural village in the middle of sallow pelitic siltstone.

步氏和政羊化石大量出土于广河县阿力麻土乡后山村，产于灰黄色泥质粉砂岩中。