查询词典 stop buffer

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

This invention discloses a multi-queue sequence buffer management circuit and a method based on a pipeline applying a pipeline structure including: an arbitration circuit selecting one for process from read, write and distribution buffer requests, a buffer slot state module designing state of the slot requiring operation and queue numbers and assigning idle slots, a buffer slot filter module filtering the slot, a buffer slot filter module filtering the slot states not belonging to the current operation queues nor idle aligned in terms of the head pointer, a queue slot selection module computing continuous idle slot numbers from the slot pointed by the head pointer and refreshing the head pointer and selecting preparing slots, a queue slot prior queuing module refreshing the read pointer and result numbers of the current operation queues with the pointer of the first prepare slots and their numbers which can support multi-queue to share one buffer space, queues can access the buffer in overlap.

本发明公开一种基于流水线的多队列顺序化缓冲管理电路及方法，本发明电路采用流水线结构，包括：仲裁电路，从读、写、分配缓冲请求中选取一路进行处理；缓冲槽口状态设置模块，设置请求操作的槽口状态和队列号，分配空闲槽口；缓冲槽口滤除模块，滤除不属于当前操作队列且非空闲态的槽口状态，按头指针对齐；队列槽口选择模块，计算头指针指向槽口起的连续空闲槽口数并更新头指针，选出预备态的槽口；队列槽口优先排队模块，用第一个预备态槽口的指针和预备态槽口数分别更新当前操作队列的读指针和结果数；本发明可以支持多个队列共享一个缓冲空间，各类指令队列能对缓冲进行交叉访问，并对指令结果的写入读出进行顺序化管理。

I'll never stop I don't know, will you believe me After all is said and done All the lies, how I regret them baby Now, I am the loser And you're shining like the sun Tell me why can't I still be the one, alright I will never stop Until you're mine I can wait forever, till the end of time Cause my heart is in your hands Don't you understand I'll never stop I'll never stop How could I ever When my heart is in your hands And I know, baby there is no turning back They say that I'm crazy And I kind of understand How I wish for this nightmare to end Oh yes I will never stop Until you're mine I can wait forever, till the end of time Cause my heart is in your hands Don't you understand I'll never stop I'll never stop Ooh ooh, ooh ooh, ooh oohooh Do you believe me, when my heart is in your hands Don't you understand I'll never stop I will never stop Till the end of time Heart is in your hands I'll never stop Until you're mine I can wait forever, till the end of time Cause my heart is in your hands Don't you understand I'll never stop ..

我将从不停下来我不知道，你将相信我终归被说和做所有谎话，如何我惋惜他们婴儿现在，我是输者同时，你发光像太阳一样告诉我为什么我仍然不能是一，alright 我将从不停下来直到你是我的我永远能等待，直到时间的末端原因我的心在你的手中你不要理解我将从不停下来我将从不停下来如何能我曾经当我的心在你的手中时同时，我知道，婴儿没有往回旋转他们说我是疯狂的同时，我理解的种类我如何希望这恶梦结束啊是我将从不停下来直到你是我的我永远能等待，直到时间的末端原因我的心在你的手中你不要理解我将从不停下来我将从不停下来 Ooh ooh，ooh ooh，ooh oohooh 做当我的心在你的手中时，你相信我，你不要理解我将从不停下来我将从不停下来直到时间的末端我将从不停下来直到你是我的我永远能等待，直到时间的末端原因我的心在你的手中你不要理解我将从不停下来。。

Through a combination of T7 RNA polymerase and T7 Promoter for testing proper buffer was selected.The experimental results showed 5×binding-Buffer 1 was more suitable for the test;TBE running-buffer was better than TAE and TGE buffer;0.2×TBE buffer was the best concentration amang there electrophoresis buffer.

通过用T7RNA聚合酶与T7启动子DNA的结合反应来选择合适的缓冲液，结果表明，不同的缓冲液会影响凝胶迁移试验的结果，5×Buffer1结合缓冲液的效果较好；TBE电泳缓冲液比TAE、TGE的电泳效果好，同时在3种不同浓度的TBE缓冲液中，0.2×的TBE效果最好。

Trans-Blot Cells and Systems 170-3825 Trans-Blot Cell With Wire Electrodes and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3850 Trans-Blot System With Plate Electrodes and PowerPac HC Power Supply, 100120/220240 V 170-3853 Trans-Blot System With Plate Electrodes, Super Cooling Coil, and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3910 Trans-Blot Cell With Wire Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3939 Trans-Blot Cell With Plate Electrodes and Super Cooling Coil, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3946 Trans-Blot Cell With Plate Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) Trans-Blot Cell Accessories 170-3912 Super Cooling Coil, required for all high-intensity transfers 170-3913 Gel Holder Cassette, includes 2 fiber pads 170-3914 Fiber Pads, 15.5 x 20.5 cm, 6 170-3920 Trans-Blot Standard Wire Electrode Card, cathode 170-3921 Trans-Blot Standard Wire Electrode Card, anode 170-3922 Trans-Blot Cell Buffer Tank 170-3923 Trans-Blot Cell Lid With Power Cables 170-3943 Trans-Blot Platinum Anode Plate Electrode 170-3944 Trans-Blot Stainless-Steel Cathode Plate Electrode 170-3945 Trans-Blot Plate Electrode Pair, platinum anode and stainless-steel cathode 16 规格:说明: Trans-Blot Plus

电泳转印槽组件 1。缓冲液槽及带有电缆的盖 2。凝胶支架转印夹 3。纤维衬垫 4。电极丝 5。电极板 6。特级冷却芯 Trans-Blot 转印槽是功能灵活的转印设备，可理想地用于多种转印应用。Trans-Blot 转印槽特点包括：*能进行多胶转印，可容纳3 块PROTEAN I xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶*多组参数灵活可设，可调节的电压设置（从30 V 的过夜转印到200 V 的1 小时快速实验）*电极间距设置为8 cm 用于标准印迹杂交，或设置为4cm 用于高强度印迹杂交*可选择板式电极：涂有铂金的钛作为正极，不锈钢为负极，能提供高强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝*通过特级冷却芯和水循环仪来调节温度―是天然酶（4°C）或高强度转印的理想选择，随着转印时间增加（多达24 小时），不会引起缓冲液耗竭（在高强度转印中必须使用冷却芯，也推荐用于所有板式电极的应用）*带铰链的凝胶支架转印夹能避免滑动，确保凝胶与印迹膜间的紧密接触；每个转印夹都有颜色标记以保证在转印槽中的正确定位 Trans-Blot 转印槽的锁闭凝胶支架转印夹系统。转印夹（1）支撑凝胶（2）印迹膜（3）两侧有纤维衬垫和滤纸（4）确保凝胶三明治内的完全接触。凝胶夹垂直插入缓冲液槽中（5）。

I can't stop my love for you 2つの爱が离れてしまわないように 1つに抱いててそれでも不安な夜は访れるものだからねそんなときはギュッと大げさに抱きしめていてかけがえのないもの君がたくさんここにくれただけど何よりもいちばんかけがえのないものは君なんだ I can't stop my love for you 2人语った未来に君との居场所があるように Oh baby I can't stop my love for you 君のすべてになってねつないだ手信じて歩いてゆくと誓うよ发音: I can't stop my love for you futari katatta mirai ni kimi to no ibasho ga aru you ni Oh baby I can't stop my love for you kimi no subete ni natte ne tsunaida te shinjite aruite yuku to chikau yo futari no me no mae nazomeki hirogatta mikansei na chizu demo totemo totemo kokoro ugoita… donna kotoba narabete mite mo katarikirenai omoi kono ai ga sasayaku yo itoshisa no imi o I can't stop my love for you futari ayumu supiido wa kitto onaji hazu ja nai kara ne moshi mo kimi no tonari kono te ga hanareta toki wa miushinawanai you ni kitto mukae ni kite ne "daikirai" nante uso demo iwanai kedo "daisuki" datte uso nanka ja iwanai kara ne aenai toki sae kimi wa nani mo kangaeru yoyuu mo nai kurai kono kokoro o hitorijime ni surun dakara I can't stop my love for you futatsu no ai ga hanarete shimawanai you ni hitotsu ni daitete sore demo fuan na yoru wa otozureru mono dakara ne sonna toki wa gyutto oogesa ni dakishimete ite kakegae no nai mono kimi ga takusan koko ni kureta dakedo nani yori mo ichiban kakegae no nai mono wa kimi nan da I can't stop my love for you futari katatta mirai ni kimi to no ibasho ga aru you ni Oh baby I can't stop my love for you kimi no subete ni natte ne tsunaida te shinjite aruite yuku to chikau yo

中文歌词： I can't stop my love for you 在我俩所诉说的未来里就像有着与你共处的栖身地一般 Oh baby I can't stop my love for you 请把我当成你的全部喔相系的手相信着我走下去发誓喔在我俩的眼前像谜般扩大的未完成地图可是非常非常心动……即使我尝试着用尽所有话语都无法诉说我的思念这个爱是悄悄的诉说喔爱的意义 I can't stop my love for you 因为我俩走路的速度应该一定不会是相同的才对啊如果当你的身边这相系的手离去时为了不失散你一定得要来接我喔虽然「最讨厌你了」这句话即使说谎也不说但是就是因为「最喜欢你了」这句话虽然不是说谎也不说啊连无法见面的时间你就像都无法思考任何其它东西般因为这个心你想独占 I can't stop my love for you 为了不让两个爱分离请将它抱在一起即使如此不安的夜晚也会来临的啊到时候请你要紧紧地夸张地抱住我喔无法替代的东西你在这里给予我很多话虽如此比任何东西都还重要无法替代的东西是你 I can't stop my love for you 在我俩所诉说的未来里就像有着与你共处的栖身地一般 Oh baby I can't stop my love for you 请把我当成你的全部喔相系的手相信着我走下去发誓喔日文歌词： I Can't Stop My Love For You 2人语った未来に君との居场所があるように Oh baby I can't stop my love for you 君のすべてになってねつないだ手信じて歩いてゆくと誓うよ 2人の目の前谜めきひろがった未完成な地図でもとてもとても心动いた…どんな言叶并べてみても语りきれない想いこの爱がささやくよ爱しさの意味を I can't stop my love for you 2人歩むスピードはきっと同じはずじゃないからねもしも君のとなりこの手が离れたときは见失わないようにきっとむかえに来てね大嫌い？

The invention discloses a LCD panel, colorful filter piece and making method, which comprises the following steps: providing a base; forming a buffer material layer on the base; forming patterned lightproof layer on the buffer material layer; adopting patterned lightproof layer as lid to pattern the buffer material layer to form a patterned buffer layer; forming a separating piece through patterned lightproof layer and patterned buffer layer; making the separating piece form multiple pixel areas on the base; forming a colorful filter layer in each pixel area.

本发明公开了一种液晶显示面板、彩色滤光片及其制作方法，其中彩色滤光片的制作方法包括下列步骤：首先，提供一基板，并在基板上形成一缓冲材料层。接着在缓冲材料层上形成一图案化遮光层。以图案化遮光层为罩幕来图案化缓冲材料层而形成一图案化缓冲层，使得该图案化遮光层与图案化缓冲层共同形成一隔离件，且隔离件在基板上形成多个像素区域。然后在每一像素区域内分别形成一彩色滤光层。

Based on some related concepts of shock disturbed system, weakening buffer operators with variable weights and strengthening ones with variable weights are established respectively. This subject defines buffer operator regulation degree which reflects effect strength to original sequence caused by buffer operator, and also studie the relationship between buffer operator regulation degree and variable weight. Consequently, the monotonicity nonvariable axim of buffer operator is put forward.

基于冲击扰动系统的相关概念，提出了变权缓冲算子的概念，分别构造了变权弱化缓冲算子和变权强化缓冲算子，定义了缓冲算子调节度来反映缓冲算子对原始序列的作用强度，并研究了缓冲算子调节度与可变权重之间的关系，在此基础上提出了缓冲算子的单调性不变公理。

Based on the above ideas, I will use line buffer as a first design. And then integrate loop buffer as my second design. Finally we use instruction content bus to deliver the buffer access position as my third design. Under this design, the buffer inside CPU core can store the nested loop and reuse them.

以上述的想法做为出发，我的设计将会以line buffer做为此种缓冲器的初步设计，之后结合田滨华的loop buffer设计做更进一步的改良，最后再采用藉由指令内容汇流排来传送存取缓冲器中的指令的想法来使得CPU之中的缓冲器可以储存巢状回圈进而提升缓冲器的使用率使得指令内容汇流排的存取次数可以持续地下降。

To render and display the scene, the sample code in this step clears the back buffer to a blue color, transfers the contents of the back buffer to the front buffer, and presents the front buffer to the screen.

为了渲染和显示场景，这段示例代码在这一步中清除了背景缓存，并填充上了蓝色，将背景缓存转换到前景缓存，将前景缓存刷新到屏幕上。

I had a lovely dog named PiPi, he was one of my best friends.

我曾经有过一只小狗，它的名字叫皮皮，它很善良，它是我最忠实的朋友。

Newly ovulated mouse oocytes were cultured in the CZB medium supplemented with different concentrations of pyruvate before treated for activation or assayed for MPF activity.

我们猜测卵母细胞在不含丙酮酸的条件下老化的更快，并且卵丘细胞可能通过消耗不含葡萄糖的CZB中的丙酮酸从而促进卵母细胞老化。