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Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Primate SPAG11, the functional fusion of two ancestrally independent beta-defensin genes, produces a large family of alternatively spliced transcripts that are expressed according to tissue-specific and species-specific constraints.

灵长类动物的SPAG11融合了两个不同祖先的beta-defensins的功能，根据不同的选择性剪接形成一个大的家族，具有很强的组织特异性和物种特异性。

Rather than being selectivelyrendered anergic in vivo, circulating survivin-specific CTLswere highly functional as shown by cytotoxicity and interferongamma enzyme-linked immunospot assays in six of nine patients.Survivin-specific CD107a mobilization by T cells was found infive of five patients.

细胞毒性和γ干扰素酶联免疫印迹测定法显示，9例中有6例的外周血survivin特异性CTLs具有高度的活性，该结果与体内选择性提取时无细胞免疫反应的T细胞不同。5例患儿血标本均可检测到T细胞动员的sruvivin 特异性CD107a。

Similarly, if the strong shear, which could suppress all the scattering in the quiescent two phase region, stopped, the follow-up phase separation also displayed characteristics of anisotropism and relaxation, In this dissertation, the transposed"butterfly'and"streakpattern were observed for the first time within the shear-history-influenced phase separation, which is found closely relevant to the anisotropical relaxation behavior of macromolecules. 5. The phase separation kinetics of PS/PVME under oscillatory shear was further studied on the base of that done under simple shear. It is found that, under specific temperature and strain amplitude, the occurrence of phase separation is strongly contingent on oscillatory frequency and only intermediate frequency could effectively induce phase separation; If all considered oscillatory shear could stimulate phase separation, a fixed frequency can maintain specific most probable phase size and higher frequency yields smaller phase, which makes a higher elasticity but weaker stress relaxation; Furthermore, higher frequency can produces stronger compulsory oscillation on the MPPS but won't change the mean of the MPPS. Under given frequency and strain amplitude, the phase separation kinetics dramatically depends on the phase angle of oscillation and the phase separation corresponding to different phase angle follows different dynamical process.

在简单剪切场下相分离动力学研究的基础上进一步对振动剪切场下PS／PVME的相分离动力力学进行了尝试性的研究，发现：在一定温度和振幅条件下，相分离的发生强烈的依赖于振动频率，只有中等频率的振动剪切才能有效地促进相分离的发生；在都能使相分离发生的前提下，一定频率的剪切能够使体系维持一定的最可几相尺寸，较高频率的剪切导致体系形成的相区尺寸较小，因而体系的弹性效应较强而应力松弛效应较弱；同时，更高频率的剪切对最可几相尺寸的强迫振动效应越强，但不会使最可几相尺寸的平均值发生改变；在一定的振幅和频率条件下，相分离的动力学过程会强烈地依赖于相位角，不同相位角对应的相分离遵循不同的动力学过程但机理一样。

The Alphabet of the Ark is supposedly linked to a specific Archangelic frequency pattern, with 24 main vibratory pathways, that aligns corporeal realms with the full-Light (Metatronic-Christic) spiral in a specific path of viability relative to the core essence of Metatronic consciousness.

据推测圣经方舟字母表与一个确定的大天使频率模式连接在一起，具有24个主要的振动道路，用完美的光(Metatronic-Christic)螺旋以相对于Metatronic意识知觉的核心本质特定道路的发展力量把物质领域排列成行。

By our price and management system for conference and exhibition, we can not only offer professional and specific service for specific customers, but also ensure that customers can get the most reliable quality assurable at the most reasonable price.

公司有着国际化项目人员，策划人员，设计师，技术工人及完整的国际化专业制作工厂，我们依靠严谨的专业会展管理体系，不仅能为顾客提供全程专人专项专业服务，还确保了顾客能够以最合适的价格得到最可靠的质量保证。

The ISSR markers were fitted to a 3:1 ratio of simplex and duplex segregation for the parental alleles, as the sweet potato was expected to be autohexaploid. Fourteen and nine quantitative trait loci linked to specific ISSR markers were detected for traits in (NH×TN27) and (TN27×NH), respectively. Twenty-two of these QTL-mapped markers were unique to the specific traits, and two were linked to two traits in each linkage map. These markers appear to be applicable to crop improvement.

ISSR标识因子符合六倍体亲本对偶基因simplex与duplex的3：1分离比。14与9个与专一性ISSR标识因子连锁之数量性状基因座，分别检测NH×TN27与TN27×NH之性状，其中22个数量性状基因座图绘之标志因子，对於特殊性状而言具有特殊性；每个连锁图谱中，有两个QTL图绘之标志因子与两个性状连锁，这些标志因子应可应用於作物改良。

In order to investigate whether fragments of Sugarcane bacilliform virus were integrated into Saccharum inter-specific hybrids genome, a DNA fragment homologous to SCBV was amplified from a hybrid sugarcane plant Saccharum inter-specific hybrids var.

为研究甘蔗杆状病毒Sugarcane bacilliform virus的基因组片段是否整合进杂种甘蔗Saccharum inter-specific hybrids基因组，以采自广西甘蔗研究所的1株杂种甘蔗植株叶片总DNA为模板，根据已报道的甘蔗杆状病毒基因组序列设计多条特异引物。

Some taxon-selective primers that were intended to be specific to fungi and basidiomycetes respectively have been designed and tested to characterize fungal symbionts by amplification of specific sequences in the nuclear ribosomal unit.

大量DNA分析新技术在丛枝菌根真菌的分子遗传、分类鉴定、种间及种内亲缘关系、菌株持久性等方面得到应用，与传统菌根研究方法相比，表现出巨大的优越性。

Its essence is specific obligational subject violation specific legal benefic protected by law.

在身份犯的法律类型中，作者根据新刑法的规定，对其进行了重新的详细的分类，使之更加趋于完善与合理。

The studies on the developing of new media are very important to the increasing of the area density of HDD and to the implementation of hybrid recoding technology.

光磁混合记录方法，是一种可以突破超顺磁极限的限制，并进一步提高硬盘记录密度和读写速率的一种新型超高密度信息存储方式。

In io, mTOR inhibition promotes T cell anergy under conditions that would normally induce priming.

体内研究中，在正常可激发免疫反应的条件下，抑制mTOR可促使T细胞无能。