查询词典 specific charge

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

Include: Of history of civilization of baleful and misinterpret China, China and historical historical fact; Baleful and misinterpret his country the history, do not respect what civilization and custom are used to human civilization, his country; Derogate in cold blood, evil of figure of the anybody in doing famous book of character of revolutionary cacique, hero, important history character, China and foreign countries and famous work; Ill will derogates people army, armed police, public security and judicatory figure; Have mistreat captive, inquisition by torture to extort a confession of the clue such as convict or guilty suspect; Expression violates guilty aggressive arrogance, specific reveal criminality detail, reveal special investigation shift, expose what ought to be protected to inform against the figure such as person, witness, audio; Advertise religious ultraism, instigate between each religion, denomination, the contradiction between be religious and masses of lack of faith and conflict, harm masses emotive; Advocate sees photograph, fortune-telling, see ghost of geomantic, augur, drive treat a disease those who wait for feudal superstition activity; With evil do means to depict heavy nature calamity, contingency, horrible incident, war to wait for catastrophic occasion; Specific show licentious, rape, incestuous, love cadaver, walk the street, go whoring, of the clue such as sexual allergy, masturbation; Of the detail such as means of process of expression or obscure performance sexual behavior, sex, sex and as associated as this overmuch body contact; Show intentionally, be masked with limbs only or cover position of human body privacy with very small pall; Contain sexual suggestion, sex to tease those who wait to make the person produces a gender to associate easily; Advocate extramarital love, much horn is loved, one-night standing, sex is mistreated and change wife wait for insalubrious content; In order to grow up the film, affection lubricious film, 3 class piece, pat secretly, expose one's body, dew point reachs all sorts of flirtation to sexual written language or picture regard video program as caption or be classified; Have intense of the clue such as excitant homicide, bloody, violent, suicide, kidnap, drug taking, gamble, clever different; Have picture of excessive fright bloodcurdling, caption, background music and sound effect; Specific show animal of cause sb's death by maltreating him, catch kill, edible country protects kind of animal; Contain those who encroach individual privacy content; With affirmative, favorable fundamental key or fetching me-too means expression fights other people of affray, shame, corrupt of character bawdry; The philosophy with inactive, decadent advocate, world outlook and viewpoint of value, unwisdom of sedulous apply colours to a drawing, exaggerated nation lags behind or of social the seamy side; The cutout of the program of movie and TV that total bureau of TV of national broadcast film prohibits travelling and film, teleplay reduces part; Violate relevant law, statute of spirit.

包括：恶意曲解中华文明、中国历史和历史史实的；恶意曲解他国历史，不尊重人类文明、他国文明和风俗习惯的；蓄意贬损、恶搞革命领袖、英雄人物、重要历史人物、中外名著及名著中重要人物形象的；恶意贬损人民军队、武装警察、公安和司法形象的；有虐待俘虏、刑讯逼供罪犯或犯罪嫌疑人等情节的；表现违法犯罪嚣张气焰，具体展示犯罪行为细节，暴露特殊侦查手段，暴露应当受到保护的举报人、证人等形象、声音的；鼓吹宗教极端主义，挑起各宗教、教派之间，信教与不信教群众之间的矛盾和冲突，伤害群众感情的；宣扬看相、算命、看风水、占卜、驱鬼治病等封建迷信活动的；以恶搞方式描绘重大自然灾害、意外事故、恐怖事件、战争等灾难场面的；具体展现淫乱、强奸、乱伦、恋尸、卖淫、嫖娼、性变态、自慰等情节的；表现或隐晦表现性行为、性过程、性方式及与此关联的过多肉体接触等细节的；故意展现、仅用肢体掩盖或用很小的遮盖物掩盖人体隐私部位的；带有性暗示、性挑逗等易使人产生性联想的；宣扬婚外恋、多角恋、一夜情、性虐待和换妻等不健康内容的；以成人电影、情色电影、三级片、偷拍、走光、露点及各种挑逗性文字或图片作为视频节目标题或分类的；有强烈刺激性的凶杀、血腥、暴力、自杀、绑架、吸毒、赌博、灵异等情节的；有过度惊吓恐怖的画面、字幕、背景音乐及声音效果的；具体展示虐杀动物，捕杀、食用国家保护类动物的；带有侵犯个人隐私内容的；以肯定、赞许的基调或引人模仿的方式表现打架斗殴、羞辱他人、污言秽语的；宣扬消极、颓废的人生观、世界观和价值观，刻意渲染、夸大民族愚昧落后或社会阴暗面的；国家广播电影电视总局禁止传播的影视节目以及电影、电视剧的删减片段；违反相关法律、法规精神的。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Uses method which the field investigation sample and the indoor analysis unify, 2007-2008 years live the unicast awnless brome lawn and the awnless brome and the alfalfa interlacing in the Inner Mongolian nationality university agriculture school test farm take 2 years mix broadcast the lawn and the colleague mix broadcast the lawn as the objects of study, have studied under the different sowing seeds way the forage grass to the soil physics and chemistry nature influence, finally indicated:the change tendency which under the different sowing seeds way increases the soil bulk specific gravity overall along with the soil layer depth to present after reduces first increases, is the 10-20cm soil layer is lowest; Looking from the 0-40cm soil layer's mean value, the unicast awnless brome lawn soil bulk specific gravity is smallest, the colleague mixes broadcasts the lawn soil bulk specific gravity to be biggest.

科尔沁草原是典型的农牧交错地区，长期以来，由于不合理利用草地资源造成该区生态环境严重恶化，农牧业生产受到严重影响[1]。建立高产优质的人工草地是解决我国高寒地区草畜供求季节不平衡的矛盾，促进草地畜牧业持续发展的关键措施之一[ 2]。无芒雀麦和紫花苜蓿都是营养价值高、适口性好、抗寒抗旱能力强的多年生牧草。将这两种牧草混播不仅能提高牧草产量和品质，延长草地使用年限，还能改善牧草的营养，提高牧草的适口性，有利于调制和青贮[3]。

One of meanings of deal with concrete matters relating to work, should develop the implication of specific working; deal with concrete matters relating to work with actual and not buckish manner namely, be united in wedlock namely actual, immerse oneself in hardworking get right on the job, to specific work, adopt specific measure, obtain concrete result.

务实的含义之一，就是要用实际而不浮华的态度开展具体的工作；务实的含义之二，就是结合实际，埋头苦干实干，对具体工作，采取具体办法，取得具体效果。

The coating composition comprises, in a specific proportion, a vehicle ingredient comprising in a specific proportion an oligomer having at least twoacrylate groups and an ethylenic monomer copolymerizable with the oligomer, aacrylic-modified chlorinated polyolefin having a specific chlorine content, an organic peroxide polymerization initiator, and a polyisocyanate compound.

该涂料组合物含有特定比例的由至少具有两个丙烯酸酯基的低聚物和可与该低聚物共聚的乙烯性不饱和单体形成的媒介物成分、特定氯含率的丙烯酰基改性氯化聚烯烃、有机过氧化物聚合引发剂、和聚异氰酸酯化合物。

The study was carried out in two breeds of sheep, Indonesia Thin Tail and Merino, both infected with F. gigantica. Kinetic analysis of Fasciola gigantica specific antibody responses revealed that resistant ITT do not produce a parasite specific IgG2 response; whereas susceptible Merino sheep produce a high parasite specific IgG2 response.

以印尼短尾羊和美利奴细毛羊两种品系的绵羊为实验动物，研究动物感染巨片形吸虫后所产生的抗体依赖性细胞介导的细胞毒反应的免疫应答。

According to the successful experiences on transgenic animal production and animal cloning, gene targeting could be utilized in many fields including theory and application research on genomic modification (such as site-specific gene repair, genetic defect therapy, gene knockout, infaust gene modification, even the site-specific integration), improving expression level of specific gene in animal mammary gland bioreactors, and promoting the industrialization of transgenic animal production.

结合现有转基因动物和动物克隆技术方面的经验，利用基因打靶技术，开展动物基因组修饰的理论和应用研究，即可定点进行基因修复、治疗遗传缺陷、基因敲除失活不利基因，还可最终解决动物乳腺反应器存在的随机整合率高而表达率较低的问题，实现定点整合及乳腺特异表达，为转基因动物的产业化生产创造条件，代表了当今动物遗传学发展的主流方向。

On the other hand, on account of the accumulation of past experience and the popularity of "Valuing virtues; undervaluing penalty", law makers and executors were then capable of laying down effective regulations in connection With specific groups, specific procedures and specific situations. This marked the maturity of the Remission Policy and thereby arose such judicative customs as remission coming of suicide and substitution etc. All these embody the class nature of legislation and Judicature as well as a certain degree of reasonableness and progressiveness.

另一方面，由于秦汉时期对既往经验的总结以及汉时&尚德厌刑&的社会风尚的影响，当时法律的制定和执行者们已经能够针对特定人群、特定情节与特定情况作出相应的减免规定与决定，从而使得秦汉律法中的减免体系得到完备，并导致自杀免罪，替代免刑等若干免罪司法司惯的出现，这其中既体现了秦汉立法与司法行为所具有的阶级本质特点，又反映出其所包含的一定程度的科学性与进步性。

The General rules for the labelling of prepackaged foods "and" labelingofprepack the labeling of foods for special dietary uses lawk "in addition to the requirements of prepackaged foods bag must indicate whether the food ingredient lists, name, net, and drain matter content and content, manufacturer's name and address, production date, pasteurizd, product standards, and other items, also specifies specific marking methods, such as, the production of prepackaged foods should be clearly labeled in such a substance's specific site, the date shall be additionally nongraphite 加贴, printing or tampered with; prominent location on food labelling, clearly reflect a true food labels, moreover, the private property; on the use of preservatives in food, discharged the name " and specific provisions of the code for importing food labels to use foreign languages at the same time, you must have a close and character, and foreign language cannot be greater than the corresponding Chinese characters; all ingredients are water, no matter how much you must label, special nutritional food must mark special components and so on.

预包装食品标签通则》和《预包装特殊膳食用食品标签通则》除要求预包装食品袋上必须标明食品名称、配料清单、净含量和沥干物含量，制造者的名称和地址、生产日期、保质期、产品标准号等项目外，还规定了具体的标注方法，如，预包装食品的生产日期，应明显地标示在包装物的具体部位，日期标示不得另外加贴、补印或篡改；食品标签的醒目位置，清晰地标示反映食品真实属性的专用名称；对使用防腐剂的食品，要明确标出&具体名称&和规定代码；对于进口食品，食品标签中若同时使用外文，必须与汉字有严密的对应关系，且外文不得大于相应的汉字；所有配料甚至包括水，不论量的多少必须标注，特殊营养食品必须标注特殊成分含量等等。

Connectors are lines that can be anchored to particular places, called glue points , on the graphic object.

连接符，可固定在图形对象的特定的地方的线条。

Another way of putting it, too many agents have a say in each other's work, and bureaucratic rigor mortis sets in.

另一种表达是，太多的作用物在彼此的工作里都有话语权，官僚的僵尸开始抬头。