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Methods: By using JSB-1 with intramembranous antigen determinant as antibody, the positive rate as optimization index and MCF-7/A as model cells, cell pretreatment methods, such as the presence or Absence of FACS permeabilizing solution, the concentration of FACS permeabilizing solution, the time treating cells with FACS permeabilizing solution, the time incubating cells with JSB-1 working fluid, the time incubating cells with goat-anti-mouse IgG-FITC solution, and the conditions for keeping JSB-1 working fluid and cell numbers, were optimized.

采用抗原决定簇在细胞膜内的单抗JSB-1，以阳性百分比为优化指标，耐阿霉素人乳腺癌细胞系MCF-7/A为模型细胞，对FACS透化溶液的有无、FACS透化溶液的浓度、FACS透化溶液处理细胞的时间、鼠抗JSB-1抗体的孵育时间、羊抗鼠IgG-FITC抗体的孵育时间、JSB-1抗体工作液的保存条件、以及细胞数量进行优化。

The method includes steps:(1) dropping triethoxy - 3 - propyl silicane isocyanic acid to ethanol solution of pH sensitive reagent to obtain reaction fluid A;(2) mixing siloxane, acid, and water to obtain reaction fluid B;(3) mixing reaction fluid A, reaction fluid B and solution of calcium chloride according to proportion to obtain solution C;(4) coating solution C on micropore base of micropore plate, using sealing tape to seal micropores, and using needle to perforate each micropore at center, and protecting from light; after placing it a period of time, the method adds distilled water to micropores, keeping the back up.

本发明公开了一种微孔板pH传感器的原位制备方法，其步骤是首先将三乙氧基-3-异氰酸丙基硅烷滴入pH敏感试剂的乙醇溶液中，得反应液A；其次是将硅氧烷、酸和水混合，得反应液B；第三是取反应液A、反应液B及氯化钙溶液，按比例混合，得溶液C；第四是取溶液C涂于微孔板的微孔底部，用密封带将微孔封住，并用针在每个微孔中央刺孔，避光保存。

In 1966, Ross solved the equation of symmetrical free vibration and got six matching solution, but he believed that it is impossible to find a solution which is useful in everywhere. In 1979, Goldenveizer printed a monography in which the uniformly valid representations of curved solution were published, but the representations of membrane solution were still not found. In 1988, Ruo-jing Zhang published the uniformly valid representation of six solutions for symmetrical free vibration and eight solutions for dissymmetrical free vibration for the first time.

但却碰到巨大困难。1966年，Ross给出了轴对称振动的6个匹配解，但却认为不可能求出全域一致有效解。1979年，Gol&denveizer等出版专著，给出了弯曲解的全域一致有效表达式，但仍然没有得到6个解中奇异薄膜解的全域一致有效表达式。1988年，张若京在其博士论文中第一次给出了轴对称6个解和非轴对称8个解的全部&全域一致有效表达式。

The optimal boron concentration for growth of pea (Pisum sativum L.) andcucumber (Cucumis Sativus L.) plants was screened by designing a series of boronconcentration in nutrient solution and the influeuce of boron deficiency or toxicityon integrity of cell membrane in different organs of the two plants was studied bymeasuring pH of the nutrient solution,〓 concentration and conductance in rootexudate solution and extracting solution of different organs from the two plants.

用不同浓度硼的营养液培养豌豆、黄瓜植株，筛选和确定在温室条件下生长所需的最适硼浓度和硼毒害浓度；进而通过对缺硼、对照和硼毒害处理后营养液pH变化，根分泌物和不同组织浸提液中K+浓度和电导率的测定，研究了缺硼和硼毒害对两种植物不同组织细胞膜完整性的影响。

In the first part, first of all, we have a priori estimate for solution of the reaction-diffusion equation with initial condition and Dirichret boundary conditions. Then, we construct a semidiscrete Legendre pseudospectral scheme, and have a priori estimate for approximate solution. We discuss the asymptotic stability of approximate solution. At last, we get a error estimation on large time for solution of the semidiscrete system.

首先，对此类方程初边值问题的解进行了先验估计；然后建立方程半离散的Legendre拟谱格式，并对近似解进行了先验估计，讨论了近似解的渐近稳定性；最后，得到了半离散系统解的长时间误差估计。

Using New Zealand rabbit ears cartilage makes chondrocyte suspension, Fibrinogen was mixed with D-hank's makes a final fibrinogen concentration of 50mg/ml mixture solution, then chondrocytes resuspend with fibrinogen solution, chondrocytes-seeded fibrinogen was mixed with thrombin (25U/ml in 40mM calcium choride) to make chondrocytes/fibrin glue polymer. Pluronic F-127 was mixed with D-hank's makes a final Pluronic F-127 concentration of 400mg/ml mixture solution, then chondrocytes resuspend with Pluronic F-127 solution to make chondrocytes/Pluronic F-127 polymer. The chondrocyte concentrations was 10 million chondrocyte/ml of polymer.

为确定纤维蛋白凝胶与Pluronic F-127在注射方式形成组织工程化软骨过程中的优劣，我们进行了如下实验：应用新西兰大白兔耳软骨获取软骨细胞并体外培养，纤维蛋白原应用D-hank's液配制为50mg/ml，然后以纤维蛋白原溶液重悬软骨细胞，再与40mM的氯化钙配制的25U/ml凝血酶混合形成软骨细胞—纤维蛋白凝胶复合物；Pluronic F-127应用D-hank's液配制为400mg/ml，同样用PluronicF-127溶液重悬软骨细胞而形成软骨细胞—Pluronic F-127凝胶复合物。

When pump starts, the lower impeller lifts the solution up step by step. And the extreme centrifugal force from the enlarged higher impeller stops the solution as it entering the higher impellr. At the same time, the full solution in the chamber stops the air mixed with the solution in the chamber to go down. This way masterly eliminates both big problems of vertical sealless pump, overflow at high pressure and bubbles at big flow.

塑宝SV泵剖面结构SV Super Series Analytical cross section SV泵剖面结构泵开始运转时，底部叶轮将液体逐步上推，待液体进入上部叶轮时，即受加大之背叶的强大阻力而无法上升，但上部叶轮所卷入液体中的空气，却受阻於水室中贮满的液体而无法下降，如此巧妙地排除了立式无轴封泵高压时溢流，或大流量时吸入空气的两大难题。

In order to enhance the miscibility between the inorganic and the organic phases,the silanol coupling agent is made from the slane VTMS and the colloid silica. Then the silanol coupling agent and nano-particles of ZnSb2O6 as the conductive filler are successfully bonded and the nano-particles of ZnSb2O6 will become more organic-like. The colloid silane-SiO2-ZnSb2O6 precursor solution is subsequently mixed with a homogeneous lsopropylalcohol solution of SR-399 acrylic monomers (Dipentaerythritol pentaacrylate) and the active free radical agent709. Under nitrogen purging, this reaction solution prepolymerize at 60℃. Then, the solution is spin-coated with the spin coator at room temperature. At last, the coated thin film is cured and crosslinked by the UV curing reactor to form the highly transparent thin film with good optoelectronic properties from hybrid organic-inorganic nano-composite.

首先以矽氧烷VTMS和colloid silica制备矽氧偶合剂，再以此偶合剂对分散於异丙醇溶液之奈米级的colloid ZnSb2O6做有机化改质，以增进其与有机高分子基材的键结相容性，我们将此colloid silane-SiO2 - ZnSb2O6前驱物与SR-399有机基材单体(Dipentaerythritol pentaacrylate)的异丙醇溶液与活性自由基光起始剂709均匀掺混后，在充满氮气反应系统下，加热至60℃进行预聚合反应，接著在室温下以旋转涂布机旋转涂布成厚度均匀薄膜，最后将此薄膜直接高温硬化或以UV硬化机网状键结硬化成透明与具部份导电性等光电特性之无机/有机奈米混成薄膜。

Also, the type of dispersion of four gasliquid-liquid systems: air-decane-aqueous solution of sodium sulfite and surfactant, airmixture of decane and dodecene-aqueous solution of rhodium complex, ligand, and surfactant, air-mixture of decane, dodecene and tridecyl aldehyde-aqueous solution of rhodium complex, ligand and surfactant, and air-mixture of decane and dodeceneaqueous solution of trisodium salt of tri (m-sulfophenyl) phosphine, and surfactant is determined by electric conductivity.

本文采用电导率测量的方法实验研究了空气—正癸烷—亚硫酸钠和十六烷基三甲基溴化铵水溶液、空气—正癸烷和1-十二碳烯混合物—铑膦络合物催化剂和十六烷基三甲基溴化铵水溶液、空气—正癸烷、1-十二碳烯和十三醛混合物—铑膦络合物催化剂和十六烷基三甲基溴化铵水溶液、空气—正癸烷和1-十二碳烯混合物—三间磺化苯基膦三钠盐和十六烷基三甲基溴化铵水溶液四个三相体系的液液分散特性。当有机相与水相体积比分别为7／3、1／1和3／7时，前三个体系均形成水包油型分散，并有稳定的乳状液生成。

The results show that the perfect Honeycomb pattern films without defects within area of 0.8cm^2 can be obtained at the PS concentration ranges from 20 mg/mL to 50 mg/mL and PS/TEOS mass ratio less than 2. The films cast from the chloroform solution of dicarboxyl terminated PS were more perfect than those cast from the chloroform solution of monocarboxyl terminated PS. The perfect Honeycomb structure films could be obtained with both chloroform and benzene as solvents, but the pore diameter of films cast from benzene solution of PS was larger than that of films cast from chloroform solution of PS.

研究结果表明，聚合物浓度在20~50mg/mL，PS/TEOS质量比大于2：1的条件下可以制备结构完整的多孔膜，且制备的Honeycomb结构在0.8平方公分内无缺陷；采用双羧基封端的聚苯乙烯成膜效果好于单羧基封端的成膜效果；以氯仿和苯作为溶剂均可以制备完全Honeycomb结构的复合膜，但以苯为溶剂制备的多孔膜孔径较大。

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。