查询词典 sera

TTV DNA isolated from sera of 6 hemophilic patients by polymerase chain reaction were cloned using TA easy vector.

目的 了解血友病人的输血传播病毒基因型及其在个体内存在状况。

TTV DNA isolated from sera of 6 hemophilic patients by polymerase chain reaction were cloned using TA easy vector. 3-5 clones selected from each patient randomly were sequenced, and then compared with TTV DNA sequences in GenBank.

对6例血友病人血清中分离的TTV进行基因克隆，从每例病人的标本随机选多个克隆进行测序，比较其核苷酸和氨基酸序列。

Experimental results show that the proposed method can be used to prepare high titer and specific rabbit anti-Humulus pollen protein polyclonal antibody. It has similar ability in determining the composition of Humulus pollen allergic protein compared with sera of patients. Therefore, it can be used to immunoscreen cDNA library.

制备的兔抗血清具有较高效价和较好的特异性，应用该血清筛选出的葎草花粉致敏蛋白组分与患者血清筛选的组分相似，因此可进一步应用于初筛cDNA表达文库。

The specificity of polyclonal antibody was detected with Western blotting, which applied rabbit antiserum and sera of known Humulus pollen allergic patients.

同时对葎草花粉过敏性哮喘患者血清进行Western blotting检测。

Methods The in vitro cultured porcine vascular endothelial cells and human sera were co-reacted to set up an in vitro model of hyperacute rejection of pig-to-human xenotransplantation.

用体外培养的猪血管内皮细胞和不同人的血清共同反应，建立猪与人异种移植超急性排斥反应的体外实验模型。

Western-blot analysis and indirect immunofluorescence assay showed that the gp90 protein expressed successfully was 45ku in molecular weight,possessed good reactionogenicity and could be recognized by mouse hyperimmune sera against the gp90 protein under natural or denatured state.

抗gp90蛋白小鼠超免疫血清介导的Western-blot分析和间接免疫荧光试验结果显示，gp90蛋白被正确表达，分子质量约为45ku；gp90蛋白在变性和自然状态下均能够被识别，具有良好的生物活性。

Nucleoprotein is also a major immunogenetic antigen for SARS; however, several tests have shown that N protein had the reactivity with other coronavirus positive sera. It is therefore an advantage of S protein as recombinant target antigen for SARS serum diagnosis compared with recombinant N protein. Moreover, S protein has been shown to be the major antigenic determinant that induces neutralizing antibodies and protective immunity to SARS-CoV, so it is also important in designing SARS genetic engineering vaccine.

SARS-CoV S 蛋白由1255 个氨基酸组成，体外表达如此大的蛋白很困难，而且表达蛋白的可溶性不高；其次，实验已经证实，全长S 蛋白含有大量无关的表位，致使表达的全长S蛋白对SARS 阳性血清的检出率低于S 蛋白片段；另外作为疫苗研究，S 蛋白上大量无关表位作为异体蛋白进入体内，会引发机体的过度免疫反应，这也是SARS-CoV 可能的致病机理之一。

Methods With indirected immunofluorescence,ANA in sera of normal people of Han,Hasake nationatilities were detected.

应用间接免疫荧光法检测汉族、哈萨克族正常人群血清中ANA。

Pallidum-antibody with indirected-ELISA based on the fusion protein. Zealand rabbits were immunized with the fusion protein, antibodies to anti-Gpd in sera were detected with indirected ELISA.

同时用纯化的Gpd重组蛋白免疫新西兰兔，间接ELISA方法检测免疫兔血清中Gpd多克隆抗体的效价，对Gpd重组蛋白的免疫原性进行分析。

Methods Rabbits were randomized to three groups. Complex model group were fed with feed containing 0.5% cholesterol for 3 weeks. At the fourth, eighth and twelfth weeks, rabbits were given injection of lipopolysaccharide (1 μg) at auricular artery, cervical part and fold inguen, respectively. Normal group were fed with normal diet, while High cholesterol group were fed with feed confaining 0.5% cholesterol only. After 16 weeks, vascular tissues were analyzed using morphometric and histological methods, and the sera were checked for cholesterol, LDL-C, C-reaction protein and tumor necrosis factor-α. Results The cholesterol and LDL-C of High cholesterol group were increased, compared to those of normal group.

给含0.5%胆固醇的饲料，3周后，分别在第4、8、12周采用耳动脉内、颈部、腹股沟处肌肉注射大肠埃希菌脂多糖，并设立正常组和单纯高脂组。16周后观察兔的一般状态，取血清检查血脂六项、C-反应蛋白和TNF-α，取耳动脉、颈动脉、主动脉弓、胸主动脉、腹主动脉、髂动脉、肝脏，放置4%多聚甲醛中过夜，常规行HE染色，检查血管病变和相关脏器病变情况。

Connectors are lines that can be anchored to particular places, called glue points , on the graphic object.

连接符，可固定在图形对象的特定的地方的线条。

Another way of putting it, too many agents have a say in each other's work, and bureaucratic rigor mortis sets in.

另一种表达是，太多的作用物在彼此的工作里都有话语权，官僚的僵尸开始抬头。