查询词典 sequence number

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

Based on the sedimentary combination, lithology and sequence characteristic, two classes of sequence structures were generalized as structure entireness and structure lackness.

并根据剖面纵向沉积组合、岩性特征及层序特征，概括出结构完全型和结构缺失型两类层序结构。

The most common approach is to consider the problem of machine equivalence which defines two synchronous design specifications functionally equivalent if, clock by clock, they produce exactly the same sequence of output signals for any valid sequence of input signals.

最常用的方法是考虑的问题，机等价定义两个同步设计规格等同功能，如时钟的时钟，它们产生完全相同的序列输出信号的任何有效的序列输入信号。

Chapter 3, in the above system, gives out the definitions of topological new transitivity, topological transitivity, topological strong transitivity and topological conjugate of a sequence of maps, studies basic properties of the above topological conjugate, and obtains some main results, proves topological new transitivity and topological transitivity are equivalent u-under a compact metric space, a sequence of full maps and interchangeable with each other, and that some conclusions associated with topological conjugate.

第三章，在上述系统中给出了一列映射的拓扑新传递、拓扑传递、拓扑强传递和拓扑共轭的定义；研究了一列映射的拓扑共轭的基本性质，得到了一些主要结果；证明了在底空间是紧致度量空间、一列映射是满映射并且两两可交换的条件下拓扑新传递和拓扑传递是等价的；还证明了几个与拓扑共轭相关的结论。

On the basis of analyzing the characteristic of the basic immune algorithm and utilizing the ergodic property of the chaotic sequence, an adaptive immune evolutionary algorithm combined with chaotic sequence is proposed and used at the training of the neural networks.

在分析基本免疫算法优化性能的基础上，利用混沌序列的遍历性，给出了一种嵌入混沌序列的自适应免疫进化算法，并将其应用于神经网络的训练中。

It is proved that a generalized Ishikawa type iteration sequence convergences to the unique common fixed point of its more generalized quasi-contractive mapping sequence respect to p.

证明了广义Ish ikawa型迭代序列收敛于关于p的更广义拟压缩映射序列的唯一公共不动点。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段，序列测定表明除585位有一点突变外，所得suc2基因与文献报道相同，构建的定位突变载体导入酵母细胞后，得到4株营养型符合的突变体，PCR表明这些突变体中均有suc2基因的同源重组，取其中1株的PCR产物进行序列测定证实了同源重组的发生。

First is the method of recognition and reconstruction of the m sequence, then how to display the frequence of the sequence is also concerned .

本文首先介绍了m序列的特性和已调伪码解调的方法。

I had a lovely dog named PiPi, he was one of my best friends.

我曾经有过一只小狗，它的名字叫皮皮，它很善良，它是我最忠实的朋友。

Newly ovulated mouse oocytes were cultured in the CZB medium supplemented with different concentrations of pyruvate before treated for activation or assayed for MPF activity.

我们猜测卵母细胞在不含丙酮酸的条件下老化的更快，并且卵丘细胞可能通过消耗不含葡萄糖的CZB中的丙酮酸从而促进卵母细胞老化。