查询词典 recombinant

After linearisation by restriction enzyme PacI, the recombinant plasmids were transfected into HEK 293-cells and amplified to give large quantities of infectious recombinant adenovirus carrying the sequences of si-FOXO1s, designated Ad-si-FOXO1-1, Ad-si-FOXO1-2, Ad-si-FOXO1-3, Ad-si-FOXO-pan and Ad-NC, respectively.

人工合成si-FOXO1s的cDNA序列，退火形成的双链cDNA克隆到腺病毒表达载体pAdTrack-CMV上，经PmeI线性化后与pAdEasy-1细胞内同源重组，最后在HEK293细胞中包装为重组腺病毒，分别命名为Ad-si-FOXO1-1、Ad-si-FOXO1-2、Ad-si-FOXO1-3、Ad-si-FOXO-pan和Ad-NC。

Pocine interferon α mature protein genes were amplified by reverse transcription polymerase chain reaction, and the recombinant replication-defective human adenovius serotype 5 plasmid pAd-poIFN-α was constructed. When the recombinant plasmid pAd-poIFN-α was linearized with PacI, and then transferred into HEK-293A cells, the virus plaque was isolated and purified in HEK-293A cells by three of plaque purification passages.

本研究利用RT-PCR方法扩增猪α干扰素成熟蛋白基因后构建了重组腺病毒质粒pAd-poIFN-α，经PacI酶切后转染HEK-293A细胞，3次噬斑纯化后获得了重组腺病毒rAd-poIFN-α。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

PAP-C23, amplified from mature pokeweed antiviral protein gene by PCR, was cloned into the expression vector PET101, and then the recombinant plasmid was transformed into Escherichia coli BL21(DE3). The recombinant mutant PAP was expressed high-levelly induced with IPTG.

用KCL溶液染SDS-PAGE胶，切下表达的目的蛋白，冰浴研磨后用磷酸缓冲液溶解，再加入等体积的福氏不完全佐剂，乳化完全后，免疫家兔，制备抗PAP蛋白的多克隆抗体，间接ELISA法测定所制备的抗血清的效价为1：1000。

Pronucleus expression recombinant pMAL-C2X-HBV-X is constructed successfully and with the IPTG induction, the recombinant pMAL-C2X-HBV-X can express the X protein in E. coli JM109, which lays the foundation fur the HBV X protein purification and its biological study.

成功构建了HBV X基因原核表达重组体pMAL-C2X-HBV X；在IPTG的诱导下，该重组体在大肠杆菌中可表达X融合蛋白，为进一步纯化HBV X蛋白和研究其生物学作用奠定了基础。

The recombinant proteins form inclusionbody in cytoplasm. Dissolve the inclusionbody with urea and refold it with metal affinity chromatography. The refold recombinant protein has certain antibacterial activity.

对表达产物进行复性，结果显示无论是融合表达的VHb-Fetidin还是非融合表达的Fetidin都无法用稀释法和尿素浓度梯度透析法成功复性，只有VHb-Fetidin可以通过金属亲和层析柱成功复性。

Tilter of the recombinant vaccinia virus is 1×109 pfu/ml.Conclusion This experiment provides a new approach to study the function of CD20 which will also lay a foundation of preparation for the mAb against CD20. human CD20;recombinant vaccinia virus;gene expression

本实验将编码人CD20的cDNA重组到痘苗病毒载体pJSA1175中，构建含人CD20编码cDNA的重组痘苗病毒，希望获得能够表达CD20的重组病毒，用于CD20分子的功能研究及单抗或多抗研制。

Abstract］ objective to observe the therapeutic effect and safety of low dose recombinant tissue-type plasminogen activator,urokinase and recombinant streptokinase in embolism therapy for acute myocardial infarction.

摘要］ 目的观察小剂量重组织型纤溶酶原激活剂、尿激酶和重组链激酶治疗急性心肌梗死的疗效和安全性。

Key words］ low dose recombinant tissue-type plasminogen activator;urokinase;recombinant streptokinase;acute myocardial infarction

关键词］小剂量重组织型纤溶酶原激活剂；尿激酶；重组链激酶；急性心肌梗死

The invention discloses a pyruvate oxidase gene derived from viridian aerococcus, recombinant expression plasmid constructed by the gene and an engineering strain of escherichia coli gene transformed by the recombinant expression plasmid.

本发明公开了一种绿色气球菌来源的丙酮酸氧化酶基因AvPyOD，该基因所构建的重组表达质粒，以及该重组表达质粒转化的大肠杆菌基因工程菌株。

Do not gum up the work by telling mother what we are going to do .

别告诉母亲我们要干的事，那会把事情搞糟的。

In recent years, application of the partial prestressed concrete beam s is developed.

近年来，随着部分预应力砼梁应用的推广，发现我国规范所采用的名义拉应力法的计算结果不稳定。