查询词典 reagent

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

The soluble peptidoglycan is gained by short dose benzylpenicillin sodium and sephadex G-100; the SLP reagent kit revealed the prepared soluble peptidoglycan is peptidoglycan; the limulus test showed that the content of LPS in the prepared soluble peptidoglycan(10ng/ml) is lower (0.01ng/ml); the improved LOWRY reagent kit showed the content of protein in the prepared soluble peptidoglycan(1.0mg/ml) was 0.06mg/ml; the optimization condition is 0.75mg/ml of the dosage of benzylpenicillin sodium,1h of the incubation time of benzylpenicillin sodium addition and 5×108CFU/ml of the bacterial concentration.

应用青霉素诱导和Sephadex G-100凝胶柱成功分离得到金葡菌可溶性PGN；所制备的可溶性PGN经SLP试剂检测结果为阳性；动态浊度鲎实验检测制备的可溶性PGN(10ng/ml)中LPS含量小于0.01ng/ml；改良LOWRY试剂检测可溶性PGN(1.0mg/ml)中蛋白含量为0.06mg/ml；青霉素诱导和Sephadex G-100凝胶柱法提取金葡菌可溶性PGN的最佳条件是：青霉素的使用浓度为0.75mg/ml，菌液浓度为5×108CFU/ml，加入青霉素后孵育时间为1h。

In all of ruthenium complex being used as CL reagent, tris-(2,2'-bipyridine) ruthenium Ru(bipy32+ is the most widely used and the analytical application of this reagent is mainly based on its electrogenerated chemiluminescence reactions.

三(2，2'-联吡啶)钌[Ru_3~(2+)]是目前最为常用的一个钌配合物类化学发光试剂，它的分析应用主要是通过电致化学发光方法实现的。

Bromate, perbromate, hypochlorite, perchlorate, ferrate,bismuthate, hydrogen peroxide, ozone, oxygen,hydroxyl radical, vanadate, ultraviolet light, bombcolorimetry, combinations of several oxidants andelectrochemical techniques. These approaches have notbeen suitable due to difficulties in reagent preparation,reagent stability, photosensitivity, low oxidationpotential, poor oxidation efficiency, expense, and userprotocols that proved too complex.

哈希实验室就试验过高锰酸盐，铈盐，过硫酸盐，过碘酸盐，碘酸盐，高铁酸盐，溴酸盐，过溴酸盐，过氧化氢，臭氧，氧，羟基，钒酸盐，紫外光，氧化弹，比色计，数种氧化剂与电化学技术的结合，这些方法已不适用，由于试剂难于制备，试剂稳定性，光敏感，低氧化电位，低氧化率，昂贵，用户证实太复杂方面的问题。

Methods The single reagent biurea reaction was altered to the double reagent and direct determination was performed by using the two-end-point method on the automated biochemical analyzer.

将单试剂双缩脲法改成双试剂，在全自动生化仪上采用二点终点法直接测定。

A series of reversible thermochromic paints were prepared using different kinds of inorganic compounds as filler,cresol red as chromophoric reagent and boric acid as delomorphic reagent.

以甲酚红为发色剂，硼酸为显色剂，选择不同的无机填料，制备出系列可逆热致变色颜料。

MethodThe extracts was prepared by grinding 800-1 000 mg fresh leaves of Clerodendron inerme and Vitex rotundifolia added with liquid nitrogen. The sample of total RNA was prepared by centrifugating the extracts added with reagent at 4 ℃, centrifugating at room temperature after ice bath and centrifugating at room temperature after adding reagent in order.

方法］取800～1 000 mg许树和单叶蔓荆的鲜叶，加液氮研磨后制成提取液，提取液加试剂4 ℃下离心，冰浴后室温下离心，依次添加试剂后室温下离心，即得total RNA样品。

Reagent of salt of sulfur cyanic acid once considered as the most effective chemistry to enhance reagent.

硫氰酸盐试剂曾经认为是最有效的化学增强试剂。

A new reagent p-chloroantipyrylazo was studied as analytical reagent for spectrophotometric determination of Ca2+ in the cytolymph of industrial microorganis.

研究了新试剂与Ca2+的显色反应，建立了测定工业微生物胞液中微量Ca2+的新方法。

The sodium bentonite was modified by organic reagent hexadecyl trimenthyl ammonium bromide and cationic polyacrylamide. The structures of modified organo-bentonite and the effect of concentration of the intercalating reagent and reacting time on decoloring capacity were studied.

用阳离子有机改性剂十六烷基三甲基溴化铵和阳离子聚丙烯酰胺复合插层改性钠基膨润土，分析了改性膨润土的结构，研究了改性剂质量浓度及二次改性时间对复合插层膨润土脱色效果的影响。

This paper designs the communication system of radio telescope FAST.

针对500m口径球面射电望远镜FAST分布式控制系统的特点完成了其通信系统的设计。

Political sheep's step-by-step a large number of fossils unearthed in the county soil Ma Ali after rural village in the middle of sallow pelitic siltstone.

步氏和政羊化石大量出土于广河县阿力麻土乡后山村，产于灰黄色泥质粉砂岩中。