查询词典 pinch effect

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The formula was established about calculating design parameters and technological parameters of ideal strip and bent strip in the period of forming. The thesis presented how to calculate the deformed area length and contact are radius of contact deforming between two elastic bodies on condition of the friction. The calculating formula of pinch force about plate-strip pinch mill was given and the results were analyzed that friction coefficient and radius of pinch rolls are very important for determining pinch force. Moreover, the rule of deforming and stress distribution of different 〓 ratio were gained on condition that the deforming was considered to be non-uniform distribution in the gap. The differential equations were got that calculate the displacements of large deflection of elastic cantilever rectangle plate with fixed end and the formula of the Finite Strip Method was conducted to study the regularity of mid-plane displacements, deformation and stresses for elastic-plastic large deflection bend of cantilever parallelogram plate with fixed or slidable end by integrating Prandtl-Reuss equation.

建立了一套完整的设计参数计算公式和在成型过程中理想带钢或具有月牙弯带钢的工艺参数的调整公式；在考虑摩擦力的条件下，对两弹性体接触变形过程中的接触变形区长度和接触弧半径用位移解法进行了研究，推导了板带递送机的递送力计算公式，从而得知在递送机设计过程中，应选择适当摩擦系数和辊身直径；另外，还从辊缝内带钢实际变形出发，分析了对于不同的〓值，带钢的变形规律及应力分布状况；建立了固定端悬臂矩形板弹性大挠度弯曲变形的控制方程，并且在对Prandtl-Reuss方程的积分处理的基础上，导出了固定端和滑动端悬臂平行四边形薄板的弹塑性大挠度的中面伸展位移、变形和应力的有限条元法公式，编写了Fortran语言计算程序，在各种参数条件下，对螺旋焊管成型过程变形规律进行了全面的分析研究，从而为螺旋焊管的生产和进一步的改进提供了理论依据。

The making technology of the brick carving of Linxia is divided into two kinds,pinch * and * carve ". The meaning of " pinch * is to pinch the ooze: Pinch the clay and mud with hands or the mould at first, the mud is pressed into various kinds of pattern, then put the mud into the kiln, fire it for the finished product.

捏活就是捏软泥，即先把配制好的粘土泥巴用手工或模具捏制、倒压成各种图案，然后入窑烧制为成品，作为建筑材料，主要用于屋脊、墙面、墙角等处使用。

Buy 300 coins, gold guess over a hundred, two hundred silver hit, pinch pig firewood burner dog, cat wells Tam hard edge, hard non-steady, falling well doused with a dry fire Tam Tong Ling, shrimp jumped pinch slope, pinch Yutiao pan into the fire.

出售300枚金币，黄金猜超过100 ， 200银击中，捏猪木柴燃烧的狗，猫井潭硬边，硬非稳态，远远浇上干火灾谭童梨嗯，虾跳捏斜坡，捏鱼跳潘纳入火灾。

The operating pinch point was studied for a given HEN, and the change of operating pinch point was qualitatively analyzed.Bypass optimal control was proposed and the bypasses were placed around the operating pinch point.

本文从操作的角度求解并分析换热网络结构已定或网络正在运行情况下的操作夹点，定性分析操作夹点的变化规律，并提出在操作夹点附近设置旁路实现网络的旁路优化控制，从而将夹点技术应用于换热网络旁路优化控制中。

He added: It wasn't about suddenly becoming Tim Burton, but it was finding a pinch of that, a pinch of Twilight, a pinch of Harry Potter - but it's still absolutely, slap-bang, mainstream Doctor Who.

他补充道：『这不是突然就变得好像添布顿一样的风格，而是从中寻找一点点好像暮光之城，哈利波特的影子，而且，最重要的是，神秘博士，仍然是不折不扣的主流神秘博士。

The effect of treatment is negatively correlated with the course of disease, generally, the effect is much worse with the course of disease is much longer; The effect is positively correlated with the course of treatment, it is better if the course of treatment is longer.The group of more than one year is much excellent than the group of less one year. The ex-electroencephalogram before treatment is normal, on edge and a little abnormal, the effect is better. As regards CT or MR result, there is difference between the effective group and the ineffective group. The result of CT and MR is negative, the effect is better, and the effect to demyelination and leukoencephalopathy is most worst.However,for the tipe of much abnormality, the effect is the most worst.The effect is related to course of disease.The effect is better if the course is shorter.There is no difference in the tipe of disease the symptoms before episode, the frequency of episode, the inducing reasons, the age, sex and so on.

病程的长短与疗效存在负相关关系，病程越长疗效越差；疗程与疗效存在正相关关系，疗程越长疗效越好；疗前脑电图的异常程度与疗效存在负相关关系，正常、边缘及轻度异常者，疗效较好，重度异常者疗效最差；无效组与有效组在CT及MR检查结果上有差异，有阴性者疗效好，阳性者疗效差，脱髓鞘及脑白质病疗效最差的趋势；疗效与性别无相关性；无效组与有效组在发作类型、既往病史、辨证分型、发作先兆、疗前发作频率、诱因、发病年龄、就诊前所用药物方面无差异。

As a whole, this article investigates the investment effect problem from a new view of regional economics. It covers the economic, social and ecological effect of regional investment; it defines the concepts of investment effect and regional investment effect, deciphers the connotation, classification and influential factors of investment effect, and establishes a basic theory framework of investment effect research; constructs a fire-new regional investment effect evaluation model that could evaluate comprehensively the economic, social and ecological effect of regional investment, including evaluation index system and evaluation method, which supplies the gap of regional investment effect evaluation in academic community.

总体上看，本文从区域经济学这一新的视角研究投资效应问题，内容涵盖了区域投资的经济效应、社会效应和生态环境效应；界定了投资效应、区域投资效应的概念，解读投资效应的内涵、分类与影响因素，建立了投资效应研究的基本理论框架；构建一个对区域投资的经济效应、社会效应和生态环境效应进行综合评价，包括评价指标体系和评价方法在内的全新的区域投资效应评价模型，弥补了学术界在区域投资效应评价方面的空白。

In the dissertation, the study on carbon fiber reinforced concrete andplain concrete has been reviewed. Then mechano-electric effect of CFRC and plainconcrete, its mechanisms, the interaction among mechano-electric effect,piezoresistivity and seebeck effect, the electrical circuit model of concrete based onmechano-electric effect, electromagnetic emission of concrete, the mathematicalmodel of mechano-electric effect are investigated. Also the converse effect ofmechano-electric effect of CFRC and plain concrete, that is, electromechanicaleffect, and the interaction between electromechanical effect and electrothermaleffect of CFRC are examined.

本文对碳纤维混凝土和素混凝土机敏性的国内外研究现状进行了全面的评述，在大量实验分析的基础上，系统地研究了碳纤维混凝土和素混凝土的力电机敏性，研究了力电效应机理、力电效应与其它效应(压阻效应、塞贝克效应等)的耦合关系、基于力电效应的混凝土电学模型、混凝土电磁发射现象、混凝土力电效应的数学模型等；论文同时对混凝土和素混凝土的电力效应、碳纤维混凝土电力效应与电热效应的耦合关系等进行了探讨；最后开展了基于力电效应的机敏混凝土结构应用研究。

Wash the lentils, place them in a pot: cover with cold water, a carrot, celery, a pinch of salt, and a pinch of fennel seed, boil covered for one hour and half at medium heat.

把洗好的扁豆放在锅里面，用冷水淹没，加入胡萝卜一个、芹菜一个、适量的盐、适量的茴香籽，煮沸后保持中热（不明白中热是什么意思，可能是指炖吧）一个半小时。

The system can be widely used in belt weighing and batching process control,etc.

介绍了基于智能模糊控制的嵌入式配料系统的设计方法，对模糊PID控制过程和系统调零作了重点的讨论，同时给出了PID控制的程序流程图和系统定长调零子程序。

"I dunno," she answered,"but if you show me aroun', I'll recognize it from the lighthouse just outside the porthole."

"我不知道，"她回答说，"如果你能带我转转，我能认出来的，因为灯塔就在舷窗外。"