查询词典 partial antigen

Coli can overproduce 38 000 antigen of Mycobacterium tuberculosis. Inclusion bodies are easy to purify and can protect the recombinant antigen from protease, on the other hand, it has not biological activity unless the denatured protein is accurately folded.

构建的大肠杆菌重组体能高效表达结核分支杆菌38 000蛋白质抗原，包涵体的表达形式有利于蛋白质纯化和蛋白质稳定，蛋白质必须经过正确折叠才具有生物学活性。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

DC takes up antigen through phagocytosis, pinocytosis, and endocytosis via different groups of receptor families, such as C-type lectin receptors for antigen capture and presentation, Toll-like receptors for pathogen recognition and DC activation.

DC通过不同的受体吞饮、吞噬和胞吞抗原，例如C型凝集素受体捕获和呈递抗原，通过Ton样受体识别病原体和激活DC。

Results Same conservative sites and key catalytic sites existed among SjLDH and LDHs from other species. Similarity of SjLDH compared to CsLDH, TvLDH and HsLDH was 75%, 17%, 58%-60% respectively. Phylogenetic analysis demonstrated that the evolution relation between SjLDH and DmLDH was closer than the relation between SjLDH and CeLDH, the relationship between SjLDH and HsLDH-B,-C was closer than HsLDH-A. Three trans-membrane regions were found, the region of 98aa-106aa in three hydrophilia regions located outside of membrane was inferred as the major antigen epitope. This antigen epitope had significant difference with LDHs from protozoon （ Pf ., Tg ., Tv .） and had 1-3 amino acid residue difference with MmLDH, HsLDH-A,-B,-C, and was the same with CsLDH. One of the key catalytic residues and substrate binding loop were located in this region. Tertiary structure demonstrated that 98aa-106aa was on the surface of the protein and formed a substrate binding loop, other two key catalytic sites were at the position near the loop.

结果 SjLDH与其他物种的同源序列含相同的保守位点及催化活性位点，与华支睾吸虫LDH同源性最高为75％，与阴道毛滴虫LDH同源性最低为17%，与人LDH（HsLDH-A，-B，-C）的同源性为58%~60％； SjLDH与果蝇的进化距离较秀丽隐杆线虫为近，3种人LDH中与HsLDH-B、-C的进化距离较HsLDH-A为近；该蛋白具有3个跨膜区域，3个高亲水性区域，主要的线性表位98aa~106aa位于膜外，与原虫LDH相同区域差异显著，而与其他LDH有1~3个氨基酸的差异，关键催化位点之一及底物丙酮酸结合区域位于该区域，蛋白质同源模建分析表明该区域位于蛋白表面形成环状结构，3个关键催化位点位于该区域或在其附近。

The present invention provides a diagnosis antigen for the serodiagnosis of bovine rotavirus diarrhea, and the diagnosis antigen has extremely strong specificity and accuracy.

本发明为血清学方法诊断犊牛轮状病毒腹泻提供了诊断抗原，具有极强的特异性和准确性。

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA，在含有甲醛的琼脂糖凝胶电泳后，转印到硝酸纤维膜上。

objective: to evaluate the diagnosis and prognosis value of serum prostate specific antigen and prostate specific antigen density in patients with prostatic adenocarcinoma.

目的：探讨血清前列腺特异抗原、前列腺特异抗原密度在前列腺癌诊断及预后判定中的价值。

Bullous pemphigoid is an acquired autoimmune subepidermal blistering disease caused by autoantibodies against bullous pemphigoid antigen 1 (BPAg1, 230kD) and bullous pemphigoid antigen 2 (BPAg2, 180kD).

类天疱疮乃因体內產生对抗BPAg1(BP230)及BPAg2(BP180)的自体免疫抗体所引起的后天性表皮下水疱病变，其好发於老年人族群。

HCV core antigen and TMV CP were detected in the systemically infected tobacco by ELISA and Western blotting . Similar expression of HCV core antigen on serial passages suggested that the complementation system of TMV-based vectors was stable in systemic infection and expression.

通过比较它们在侵染叶片中HCV核心抗原的积累水平，我们发现TMV外壳蛋白基因的5，端序列具有增强其表达的功能，其作用机制是它的起始区IV比起始区V具有更强的起始翻译功能。

Reporter molecules (thiophenol, bipyridine derivates, cyanopyridine derivates) were attached to different immuno-gold colloids, respectively. By means of the specific adsorption between antigen and antibody, a sandwich structure was constructed, in which labelled immuno-gold colloids and antigen covalently bound to substrate specifically capture different antibodies.

以多种不同的标记分子(苯硫酚，联吡啶类分子，氰基吡啶类分子)分别标记多种不同免疫金溶胶，通过抗体抗原之间所具有的特异吸附性，进一步组装"固相抗体-待测抗原-标记免疫金溶胶"多组分三明治复合体系。

Sodium citrate; calcium carbonate; calcium hydroxide; potassium bicorbonate; magnesium carbonate; ferrous sulphate; Zinc sulphate; copper sulphate; manganese sulphate; potassium iodide; sodium selenite.

柠檬酸钠；碳酸钙；氢氧化钙；碳酸氢钾；碳酸镁；硫酸亚铁；硫酸锌；硫酸铜；硫酸锰；碘化钾；亚硒酸钠。

All this to say that I'm glad filmmaking hasn't become completely ironic. I can enjoy an ironic film as much as the next person, but they usually involve a message of some sort. One that's delivered in a smirkingly clever way that requires little in the way of talent.

所有这些都是说，我很高兴电影制作没有变成完全讽刺艺术，我可以和下一个人一样享受讽刺电影的乐趣，虽然他们经常会被划归到一定级别，技术方面不需要很高的才能。