查询词典 operon

Recombinant plasmid pFf-22-Trp operon was successfully constructed, and both the anthranilate synthase and tryptophan synthase activities of expressed product increased. It laid a foundation of construction of recombinant E. coli strain for production of tryptophan in a large scale.

已成功构建了重组表达质粒pET-22b-Trp operon，邻氨基苯甲酸合成酶和色氨酸合成酶的活性在大肠杆菌中得到了提高，为高产色氨酸基因工程菌的构建奠定了基础。

Operon containing genes that metabolize the sugar arabinose.

含有阿拉伯糖代谢基因的操纵子。

Ara operon Operon containing genes that metabolize the sugar arabinose.

ara 操纵子含有阿拉伯糖代谢基因的操纵子。

ResultsUsing this method, we successfully cloned the 11kb virB operon of Brucella and constructed complementary plasmid.

我们利用该方法克隆了布鲁氏菌中长度为11kb的virB操纵子，并构建了互补质粒。

The lac operon is an inducible operon, whereas the trp operon is a repressible operon.

乳糖操纵元是可诱导的操纵元；色氨酸操纵元是可阻遏的操纵元。

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

Besides characterisation of the two genes' function, RT-PCR, Northern analysis, primer extension, and lacZ reporter gene fusions were also tried in this study to investigate the genetic organization of the operons related and their transcription regulation, map gene was found to locate at the end of a long ribosome protein genes operon and yflG is in another operon with its downstream gene.

除了对这两个基因的功能鉴定，本文还阐明了它们各自所在操纵子的遗传结构，并采用RT-PCR、Northern印迹及引物延伸等多种方法，最终分别对它们的启动子进行了定位。

The study of compared effectual primers 3′ end sequences between RAPD analysis used in tomato and Operon system.

对番茄RAPD分析的有效引物与Operon引物系列的3′端序列作了统计比较。

Ethambutol is a first-line drug used for antituberculosis therapy. It is often used in combination with isoniazid, rifampin, pyrazinamide, and streptomycin. The action of EMB in antituberculosis mechanism is the destruction of cell wall of tubercle bacilli via binding the target molecule -- arabinosyl transferases. These enzymes are involved in the polymerization of the cell wall arabinan. Inhibition of arabinan synthesis by EMB results in the accumulation of mycolic acids, leading to cell death.The EMB-resistance of MTB is involved with the mutation of emb operon or the over expression of emb operon.

乙胺丁醇是和异烟肼、利福平、链霉素、吡嗪酰胺联合治疗结核病的一线抗结核药物，EMB对结核分枝杆菌的作用机制与分枝杆菌胞壁结构破坏有关，主要作用于靶分子阿拉伯糖基转移酶，抑制了阿拉伯糖基聚合入阿拉伯半乳聚糖，从而影响细胞壁分枝菌酸-阿拉伯半乳聚糖-肽聚糖复合物的形成，导致菌细胞的死亡。

Of all primers, Operon-1 and Operon-10 were those whichled to the most efficient amplifications.

所有9个引物中以operon—1和operon—10的扩增效果为最好。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。