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objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

超氧化物歧化酶(superoxide dismutase,sod)是细胞体内歧化超氧阴离子自由基(o-2)的一个抗氧化酶,按其结合的金属性离子根据其中金属辅基的不同可分为4类:cu/zn-sod、mn-sod、fe-sod和ni-sod,它们通过催化超氧阴离子自由基0-2发生歧化反应,达到清除o-2的效果,具有防御氧毒性、增强机体抗辐射损伤能力、防衰老以及治疗某些肿瘤、炎症、自身免疫疾病等功效,广受国内外科研工作者的关注和重视[1]。

Pyramidal lattice vector quantization has drawn extensive attention by its promising property recently. The coding method based on zerotree coding[1] has made a great coup during the last few years.

近年来,金字塔格型矢量量化方法由于其本身的特点和性质引起了人们的广泛兴趣,而文献[1]介绍的基于零树的编码也获得了极大的成功。

The basic principles and main techniques of image data compression are presented in this article. The zerotree coding method and zero filling of rectangular block coding method are compared.

本文首先介绍图像数据压缩的基本原理和几种常见的图像压缩编码方法,比较了零树编码方法和矩形块填充编码方法的优缺点。

To date, one allele coding fast acetyltor (wild-type NAT2*4) and several mutated alleles coding for impaired acetylator activity have been identified.

NAT2基因具有高度的多态性,至今为止1个快型野生型等位基因(NAT2*4)和一些慢型野生型等位基因已被证实。

Fragments from the 239th to the 944th nucleotide of the coding regions of all actin mRNAs were amplified with two actin specific primers. Fragments from the 239th nucleotide of the coding region to the end of actin mRNAs were also amplified The electrophoresis results were compared between the macropterous and brachypterous brown planthoppers.

利用两条肌动蛋白特异性引物,扩增出了肌动蛋白基因mRNA的编码区片段,另外,将肌动蛋白特异性引物作为上游引物,将与3'锚定反转录引物部分序列相同的引物作为下游引物,扩增出了从肌动蛋白基因mRNA的编码区第239号碱基到Poly尾的这段cDNA,并在翅型之间作了比较。

This dissertation brings forward and realizes the multilevel classifiable method which is based on characters coding. Above all, this method realizes the first-grade classification by extracting enough effective characters from characters and coding them, to the others which couldn't be recognized by the first-grade classification, the method will adopt the second-grade classification using template matching to recognize these characters.

本文使用了基于特征编码的多级分类识别方法,通过给字符抽取足够多的有效的特征并给特征编码实现第一级分类,对于第一级分类后仍不能区分的字符,再进入第二级分类用模板匹配的方法最终达到区分的目的,这种方法的重点在第一级分类阶段。

This dissertation brings forward and realizes the multilevel classifiable method which is based on characters coding. Above all, this method realizes the first-grade classification by extracting enough effective characters from characters and coding them, to the others which coundn t be recognized by the first-grade classification, the method will adopt the second-grade classification using template matching to recognize these characters.

本文提出并实现了基于特征编码的多级分类识别方法,通过给字符抽取足够多的有效的特征并给特征编码实现第一级分类,对于第一级分类后仍不能区分的字符,再进入第二级分类用模板匹配的方法最终达到区分的目的,这种方法的重点在第一级分类阶段。

This paper on OFDM system used in the encoding technology for the study, the focus is on the cutting convolutional coding and coding.

本文主要对OFDM系统中所使用的编码技术进行了研究,重点介绍的是卷积编码和交织编码。

In summary, the work in this dissertation has solved several hard problems (including low compression rate, bad compression quality, and moderate coding efficiency, etc. under the condition of low band-width) of image compression coding in the data link of flighting control of cruise missiles, and provides a foundation for transferring high quality images instantly in man-in-the-loop control of the cruise missile.

总之,本文的研究工作在一定程度上解决了面向巡航导弹飞行控制数据链的图像压缩编码技术中存在的低带宽条件下,压缩率低,压缩质量差,编码效率不高等难题,为实现巡航导弹人在回路控制中实时的、高质量的图像压缩编码奠定了技术基础。

Mitochondrial DNA; non-coding region; coding region ; system performance ; heterogenicity

线粒体DNA;非编码区;编码区;系统效能;异质性

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许多人将继续选择活在二元对立性和冲突中。

I find that students of the University of Physical Education all wear sportswear at first sight.

我发现:体育大学的学生乍一看,都是穿运动衣,大家都一样

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我喜欢唱歌,但我还不愿意把它当作我的终身职业。