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NO elimination reactions are much complicated in the system of NO-CO-CO2-N2 with the existence of metallic oxides. Homogeneous reaction between NO and CO, reduction of NO by the lower valued metallic oxides, direct decomposition of NO as a catalytic reaction and catalytic reaction of NO/CO may all contribute in NO elimination.

金属氧化物存在条件下NO-CO-CO2-N2体系中,NO去除反应较为复杂,NO/CO气相直接反应、低价氧化物对NO的还原作用、金属氧化物对NO分解反应的催化作用及金属氧化物对NO/CO反应的催化作用等都可能存在。

Among the more and more intensive researches on NO transfer, a controversial issue regarding the mechanism is whether the NO group transfer from S -nitroso compounds occurs in a direct nucleophilic way where NO serves as the target or in a multi-step sequence initialed by homolysis of the S -NO bond.

NO转移反应的模拟研究日渐活跃。有关S-亚硝基化合物转移NO的机理存在两种争论,即以NO为靶心的直接亲核进攻还是由S-NO键均裂引发的多步过程。

Hoisting beam of the No.1 crane was replaced with that of No.4 crane and the brake assembly of the hoisting motor was replaced with a ship's spare, which had been sustained damage in the incident of the No.1 crane ar Port of XXX, by the crew so that the No.1 crane could be continued in normal working.

为了不影响卸货,船方将No.1起重机吊钩梁与No.4的进行调换,并由船员利用船上的备件对在XXX港No.1起重机损坏案相关的起货马达刹车部件进行了更换,使No.1

The metalloproteins which are widely distributed in vivo are very important target molecules of NO. A new Y/〓 reaction system which can form Y-NO bond has been established and has been used to measure the NO affinity of metalloporphyrins as metalloprotein models. Three kind of bond energies of the Co-NO bonds in series of cobalt nitrosyl tetraphenylporphyrins have been determined by thermodynamic cycles combined with calorimetric measurements and relevant electrochemical data.

本文以金属卟啉模拟体内重要的NO靶分子金属蛋白,通过确立新的适于量热和电化学测定的Y/〓成Y-NO键反应体系,利用合适的热力学循环,建立了新的适于金属卟啉体系的NO亲合势的测定方法,得到了苯腈中系列亚硝基四苯基钴卟啉Co-NO键的键能,即钴卟啉NO亲合势的定量标度,以表征钴卟啉的NO结合能力。

The nitric oxide released process of nitrosyl metalloporphyrins in vitro was detected with diazotization assay at room temperature, the four nitrosyl metalloporphyrinsbCo-NO、bFe-NO、a_(Co-NO、a_were good nitric oxide donors, the nitric oxide released efficiency is attained about seventy percent.

在室温下采用重氮化反应研究了这些金属卟啉-NO 配合物在水溶液中释放NO的过程,其中四种金属卟啉(bCo-NO、bFe-NO、aCo-NO、aFe-NO)有较好的释放效果,释放率达到近70%。

Result:① In NC group,the levels of plasmic and lymphocytic NO were higher in the morning than those in the afternoon.② The plasmic and lymphocytic NO were significantly decreased in CAD patients then those in NC group (P<0.01).③The physiological NO curve disappeard in CAD patients.The concentrations were no obvious changes and maintained lower levels in 24 hours.Conclusion:As similar as other endocrinic hormones,the NO secretion was not equal in 24 hours.

结果:①正常人24 h血浆和淋巴细胞中NO分泌呈上午高、下午低的趋势,晨6时最高,下午6时呈低谷,晚10时起又渐升高至早晨;②CAD心绞痛患者血浆和淋巴细胞中NO含量明显低于正常人,其差异具有统计学意义(P<0.01);③CAD患者失去了正常的NO生理性分泌曲线,血浆和淋巴细胞中24 h均在较低水平,波动范围不大。

Methods: NO-3 was restored with cadmium column assay and NO-2 was detected with leavy nitrogen assay. The primitive NO-3 and total restored NO-2(NO-3/NO-2) in plasma of patients with chronic hepatitis and cirrhosis as well as ET-1 with radioimmunology, ALT with Lai's assay were determinated.

取慢性乙型肝炎及活动性肝硬化患者血浆,用镉柱法还原NO-3,重氮法测NO-2,计算血浆中原有及NO-3还原后NO-2总和(NO-2/NO-3),放免法测ET-1和赖氏法测定ALT。

This conclusion is based on three groups of experiments with detached root tips of wheat seedlings: By following the time course changes of NO synthase , superoxide synthase and ABA accumulation under dehydration stress, we found that ABA accumulation was preceeded by the changes of NOS and superoxide synthase. ABA accumulation induced by dehydtrion stress and osmotic stress can be blocked by NO scanvegers and NOS inhibitors, while only dehydration stress-induced ABA accumulation can be blocked by ROS scanvegers. Exogenous applied NO or ROS induced ABA accumulation in root tips of wheat seedlings, and synergistic effects were found between NO and NO. The in planta expereiments also proved that ROS and NO are involved in water stress-induced ABA accumulation.Ⅱ.

证据主要来自以离体根尖为材料的实验:(1)通过检测干旱胁迫下小麦根尖NOS活性、O〓合成酶活性和ABA积累的时间变化进程,发现ABA积累滞后于ROS和NO的变化;(2)抑制剂实验表明,NO清除剂和NOS抑制剂可以抑制自然干旱胁迫及渗透胁迫诱导的小麦根尖ABA积累,ROS清除剂抑制自然干旱胁迫诱导的ABA积累而不抑制渗透胁迫诱导的小麦根尖ABA积累;(3)在非胁迫条件下施加外源的ROS或NO可以诱导小麦根尖积累ABA,ROS和NO之间具有协同作用。

The specimens were collected from 4 different origins: 1 Smuggled sturgeons (Nos. 1-15); 2 Aquaculture specimens from Chupei station of Freshwater Aquaculture Research Center, including A. schrenckii (Nos.18, 21-24), A. gueldenstaedtii (No.16), A. sinensis (No.17), A. baerii (No.19) and one unknown species sturgeon (No. 20); 3 A. schrenckii (No.25) and A. transmontanus (No.26) from aquaculture pond in Fu-San; and 4 Polyodon spathula (No.27) and unknown species sturgeons (Nos.28-31) from Taipei city Zoo.

研究材料取自四个地点:1海巡署在云林箔子寮港查获的走私鲟(Nos.1-15),2竹北淡水繁养殖研究中心所饲养的史氏鲟(Nos.18, 21-24)、俄罗斯鲟(No.16)、中华鲟(No.17)、西伯利亚鲟(No.19)、及未知种鲟(No.20),3福山养鳟场所饲养的史氏鲟(No.25)及高首鲟(No.26),以及4台北市立动物园所饲养的匙吻鲟(No.27)及未知种鲟(Nos.28-31)。

The results implied that NO/ROS-induced ABA accumulation come from de novo synthesis pathway rather than degration of conjugated forms. The protein synthesis inhibitor actidone blocked the NO-induced ABA accumulation while having no effects on ROS-induced ABA accumulation, indicating that the action of NO was gene-expression dependent and that of ROS was gene-expression independent. These results suggested that NO may serve as a stress signal in the regulation of ABA-biosynthesis and ROS may serve as substrate in ABA biosynthesis or be involved in the regulation of enzyme activities.

1ABA合成抑制剂fluridone抑制ROS和NO诱导的ABA积累,表明NO/ROS诱导的ABA积累来源于ABA的从新合成而不是已有结合态ABA的代谢;(2)蛋白质合成抑制剂环己亚胺可以抑制NO诱导的ABA积累而对ROS诱导的ABA积累没有影响,说明NO的作用涉及基因表达而ROS的作用与基因表达无关,暗示NO的作用方式可能是作为一种干旱胁迫信号物质参与ABA合成的调节,而ROS则可能参与了相关酶活性的调节或直接参与了ABA合成的底物反应。

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