查询词典 multiple alleles

In 110 Xinjiang unrelated healthy Uygur subjects, 27 alleles were detected in HLA-B loci. Of them, B * 35 and B * 51 were the most common alleles with an allele frequency of 0.1136 and 0.1136, respectively; while B * 41, B * 56 and B * 78 were the rare alleles with a frequency of 0.0045, 0.0045 and 0.0091, respectively. Frequencies of "Caucasoid origin" HLA alleles such as B * 08, B * 35 and B * 50 in Xinjiang Uygurs were higher than other ethnic groups in China.

在维吾尔族样品中共检出27个等位基因，其中高频率的等位基因为B*35(0.1136)和B*51(0.1136)，低频率的等位基因为B*41(0.0045)、B*56(0.0045)和B*78(0.0091)，B*08、B*35、B*50等"高加索人"起源的HLA基因在新疆维吾尔族的分布频率高于国内其他民族，与高加索人种的数值相当。

The frequencies of CCR5delta32,CCR2b-64I,SDF1-3′A alleles in Dai population were 0.0000,0.2130,0.2030,respectively;The frequencies of CCR5delta32,CCR2b-64I,SDF1-3′A alleles in Chingpaw population were 0.000,0.1637,0.1770,respectively.Distributions of the CCR2b-64I,SDF1-3′A alleles among the both populations were in accordance with Hardy-Weinberg equilibrium.No statistical difference was found in the allelic frequencies of both CCR2b-64I and SDF1-3′A between male and female individuals.

结果表明，中国傣族景颇族人群中未发现CCR5△32等位基因突变；傣族CCR2b-64I、SDF1-3′A基因突变频率分别为0.2130和0.2030，景颇族CCR2b-64I和SDF1-3′A基因突变频率分别为0.1637和0.1770；与中国汉族人群相比较，傣族和景颇族中SDF1-3′A突变频率较低（P值分别为0.0322和0.0021）；两个民族的CCR2b-64I和SDF1-3′A等位基因群体分布符合Hardy-Weinberg平衡，在性别之间分布无显著差异。

To inquire into association between susceptibility to AIDP, AMAN and frequencies of HLA alleles and possible differencies in distribution of HLA alleles betwen AIDP and AMAN by genomic typing for HLA-class Ⅰ, class Ⅱ alleles, and to try to found out the effects of self intrinsic fators of GBS patients on occurence of two subtype from immunogenetic angle.

通过对AIDP和AMAN两种GBS亚型的HLA-Ⅰ类和Ⅱ类等位基因的分型，探讨两种亚型的易感性与HLA等位基因分型的关系以及两种亚型在HLA等位基因分布上可能存在的差异，从免疫遗传学角度寻找自身内在因素在GBS不同亚型发病中的可能作用。

The novel PCR technology can accurately categorize the spectrum of FMR1 alleles, including alleles previously considered too large to amplify; reproducibly detect low abundance full mutation alleles; and correctly infer homozygosity in female samples, thus greatly reducing the need for sample reflexing to Southern blotting.

这种新型的PCR技术可以准确判断FMR1基因的类型，包括传统PCR不能扩增的大片段。该法还能检测低浓度的全突变条带，并且重复性好。同时该法还能准确判断女性样本中的纯合子，因此大大减少了需要Southern杂交的样本量。

To investigate the methods to effectively and simply assess the CAG repeat size of HD gene which was necessary for gene diagnosis of Huntington disease, the sequence including polymorphic CAG repeat of HD gene was amplified by PCR with TaKaRa LA Taq DNA polymerase and GC buffer. PCR products were analyzed on polyacrylamide gel to distinguish normal alleles from HD alleles. The DNA fragments of affected alleles were recovered from polyacrylamide gel as templets for secondary PCR. The secondary PCR products were cloned into T vector for sequencing analysis to determine CAG repeat size. A total of 20 normal individuals and 3 members from a HD pedigree were included in this study.

为了简单高效检测HD基因开放阅读框5'端n三核苷酸重复序列，建立快速准确的亨廷顿病(Huntington disease， HD)基因诊断方法，应用TaKaRa LA Taq DNA聚合酶配合GC buffer扩增HD基因包含n重复序列的目的片段，非变性聚丙烯酰胺凝胶电泳检测后回收n拷贝数异常增多的目的片段，再次PCR扩增后将产物连接至T载体，进行DNA测序确定CAG的拷贝数。

The result showed that HMW-GS was controlled by multiple alleles, and HMW-GS in F1 generation had codominance, Then the multiple alleles of Glu-1 site were codominant with two parents dominant together in F1 generation grain, them was a difference between original crass and reciprocal cross.

结果表明，HMW-GS由复等位基因控制，F1代HMW-GS具有共显性。

The allelic diversity in terms of number of alleles per locus and expected heterozygosities in cultured populations N=8.0-9.4, H(subscript e=0.754-0.787 was considerably lower than that found in the wild populations N=11.3-15.0, H(subscript e=0.821-0.866. Allele distributions at all seven microsatellites indicated that the cultured populations lost some rare alleles belonging to wild populations. The small number and the biased sex ratio of abalone for hatchery broodstock might be responsible for the remarkable reduction of genetic variation in the studied populations.

结果表明，从等位基因数与杂合度上分析，养殖群体(N=8.0~9.4， H=0.754~0.787)遗传多样性显著低于野生群体N=11.3~15.0， H(下标 e=0.821~0.866；等位基因频率分布揭示出野生群体中一些稀有等位基因在养殖群体中有所丢失；亲鲍选用数量少与性别比例不均衡可能是产生养殖群体中遗传多样性显著减少的原因。

The results showed that:(1) we can mapping quantitative trait locus while estimating the variance component of QTL;(2) granddaughter design is better than daughter design when mapping QTL;(3) it is easy to map a QTL for trait with a high heriability and a large QTL variance contribution;(4) we can estimate the variance component of a QTL by TM-BLUP based on ML method whether the QTL has only 2 alleles or QTL has normal distributed alleles effects;(5) the estimation accuracy of variance component contributed by QTL was improved by using of grand daughter design;(6) the higher the heritability and the QTL variance contribution was, the more accurate estimation of QTL variance component.

结果表明：（1）采用随机QTL效应模型和最大似然法，在估计QTL方差组分的同时，能够定位QTL；（2）孙女设计与女儿设计相比，在其它因素相同时，容易检出QTL；（3）遗传力高，QTL方差贡献较大的性状，QTL检出的效果优于遗传力低，QTL方差贡献较小的性状；（4）无论QTL上有2个等位基因，还是QTL上等位基因的效应服从正态分布，都可将其看作随机效应，采用基于TM-BLUP的ML法，估计其方差组分和定位QTL；（5）QTL方差组分估计的准确性，孙女设计高于女儿设计；（6）遗传力高的性状，QTL方差贡献大的QTL，QTL方差组分估计的准确性高。

Methods Fifty pairs of fresh colorectal cancer and homologous normal tissues were genotyped with Identifiler Kit and the mutations generated in cancer tissues were determined. The mutation rates, the numbers of locus matched without identical allele (A0), 1 identical allele (A1), or 2 identical alleles (A2) and the number of total identical alleles IA(subscript n were calculated. Frequency distributions of A0, A1, A2 and IA were compared among CR-N group, unrelated individual pairs and full sibling pairs. Discrimination functions were established for individual identification from tumor tissues with discriminatory analysis.

用Identifiler系统对50对新鲜结直肠癌组织及其身源正常组织组进行STR分型，计算CR-N组中变异STR基因座及全不同基因座数(A0)、半相同基因座数(A1)、全相同基因座数(A2)和共有等位基因数IA(下标 n，比较CR-N组、无关个体对组和全同胞对组中上述参数的分布差异，通过判别分析建立判别函数。

Gene mutations were detected in 25 patients and a 18-year-old girl among 16 families. Trinucleotide repeats of CAG were 73～79. The fragments of abnormal alleles were 380～402bp, and all patients were heterozygous. The copy numbers of normal alleles were 18～40, fragments from 202～270bp. SCV reduction was much obvious compared to MCV, MCV and SCV in lower limb ?ere much more slow than that in upper's. BAEP, VEP were also delayed in latency. The anticipation in parental sex bias were much more obvioius than that in matental's. Cerebellar ataxia was most severe, the next were dysarthria and bulging eyes. Amytrophy was seen only in bed ridden patients. Cerebellar atrophy was more severe than brain stem, cord atrophy was n't observed in all MJD.

结果检出10个家系25例病人及1例症状前18岁女孩有MJD基因突变，CAG三核苷酸重复73～79次，异常等位基因片段长380～402bp，均为杂合子；正常人CAG三核苷酸重复18～40次，等位片段长200～270bp，电生理发现MJD的SCV减慢比MCV明显，而下肢的MCV、SCV又较上肢明显，BAEP、VEP均有不同程度的潜伏期延长或波的异常；MJD的父亲遗传早于母亲，进展也较块，临床以小脑性共济失调为突出症状，其次为构音障碍、突眼等，肌肉萎缩仅见于晚期病人；MRI示小脑萎缩较明显，脑干萎缩并不严重，未见明显的颈髓萎缩。

Pradeep Indulkar, a senior official at the Maharashtra state Home Ministry say 101 human were killed and 287 injured.

马哈拉施特拉邦内政部高级官员Pradeep Indulkar称，有101人在冲突中丧生，287人受伤。

Mark Frei, a senior vice president of West, says 80% of West's homeagents have some college education, compared with 30% of those whowork in office-based call centers.

Frei说，该公司80%在家办公的人员都拥有本科学位，而在公司电话中心工作的人中只有30%拥有本科学位。