查询词典 manner of expression

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

According to these characters mentioned above,we conclude that ARVD is a autosome dominant heredity disease,And according to clinical expression of this disease,we classify these clinical expression to four type:①Arrhythmogenic type.

该病为常染色体显性遗传，可分为心律失常、右心衰竭、伴有左心病变及无临床症状四型。

In the present study, we examined the large-scale gene expression profiles with six sets of matched clinical and experimental fluconazole susceptible and resistant C. albicans strains by microarray analysis. We observed that azole resistances developed in multiple circumstances are all associated with increased expression of CDR mRNA. We also identified some common differential expressed genes and some interesting function-linked gene groups involved in drug-resistance.

利用所构建的基因芯片我们研究了共六对氟康唑敏感和耐药白念珠菌配对菌株的大规模基因表达谱，证实不同环境下产生的白念珠菌耐药性均与多药耐药基因CDR的mRNA表达增加有关，并从中鉴定了一些与药物耐受性相关的共同差异表达基因和一些有趣的功能连锁基因群。

And according the results of the fluconazole sensitivity, differential expression level of CDR1 mRNA was showed on the candida albicans azole resistance and sensitive isolates, we want to discover the molecular mechanism of CDR1 expression regulation by detecting whether there were the mutations on the upper stream of the CDR1 gene.

CDR1基因在白念珠菌唑类耐药/敏感菌株中的表达情况观察，对念珠菌的mRNA进行NORTHERN BLOT检查，发现唑类耐药株与敏感株在CDR1基因表达上存在显著差异。3。

And according the results of the fluconazole sensitivity, differential expression level of CDRl mRNA was showed on the Candida albicans azole resistance and sensitive isolates, we want to discover the molecular mechanism of CDRl expression regulation by detecting whether there were the mutations on the upper stream of the CDRl gene.

CDRI基因在白念珠菌哇类耐药／敏感菌株中的表达情况观察，对念珠菌的mRNA进行NORTHERN BLOT检查，发现哇类耐药株与敏感株在CDRI基因表达上存在显著差异。3。

As a result this gene is mixed in new student and grown bandicoot there is taller expression in the pallium of small rat and spinal cord, expression is spent in having in lung.

结果该基因在新生和成年大鼠和小鼠的大脑皮层和脊髓中有较高的表达，在肺中有中度表达。

Successful construction and expression of PEA in prokaryotic expression vector are basis for further study of genetic engineering vaccine and recombinant inununotoxin for immune target therapy.

PET-32a-PEA原核表达质粒的成功构建及表达，为进一步研究有效的基因工程疫苗和免疫导向治疗的基因重组毒素奠定了基础。

Until, before and during the culture revolution, the enthusiasm for politic had made the form of sculpting become a kind of artistic exaggeration in the vision of picture poster, in which there were three things accounted for the occurrence: firstly, the stylized shapes and composition formed an unitary expression; secondly, to be in 'high spirits' for politic must have an exaggerated and strengthened way of expression to suit with; thirdly, it was restricted by then economy and technology level so as to have to use plenty of cement as substitute, in which had only modeled on a big scale briefly.

及至文革前及文革时期，这种政治热情使雕塑语言发展成了一种夸张的宣传画式的视觉效果。其原因有三。

In the market and scientific fundamentalism bewitchment and coerced devoid of humanity self-sufficient, Tian Ran retreated to their homes, of course, there would be no dwelling of the soul, and even thought, language, and even emotional expression have been incorporated into the same institutional paradigms of "currency" of quantitative analysis, philosophy of life is no longer straight leading edge expounded the meaning of religion is no longer concerned about the limited nature of anxiety and thereby losing the salvation of the inherent features, and people still pray and not gratuitous Su the expression of piety, but most are not based on hardship of life with spiritual sustenance, but the heterogeneity of the considerations and the rather alternative pop means "faith show."

在市场与科学原教旨主义的蛊惑与裹挟之下，没有了人性自给自足、可以恬然退守的家园，当然也就不会有灵魂的栖居之所，连思想、语言、乃至情绪表达都被纳入同一性的体制范式进行"货币化"定量分析，哲学不再有直抵生命堂奥的意义阐发，宗教不再关注有限性焦虑从而失去了内在的救赎功能，人们也还在求神拜佛而且不无端肃虔敬的表情，但大多不是基于人生困厄的心灵慰藉，而是异质化的另类利害权衡和颇具波普意味的"信仰秀"。

Using the formula about the magnetic flux density induced by coil which deduced by Biot-Savart law,this paper deduces the formula about random point's magnetic flux density of hollow column solenoid and deduces the formulas about axial specific magnetic force and radial specific magnetic force by using the field gradient expression of binary function and the expression of specific magnetic force .

利用二元函数的梯度公式及比磁力公式可以得到磁性颗粒在厚壁圆环线圈所产生的磁场任意点的比磁力的轴向分量和径向分量。

His female bullfighter Lydia gradually Health love, but she was in a vegetative state after the Bulls Zhuangcheng away from her, just because Lydia's ex-boyfriend showed more affectionate than he is like love.

他对女斗牛士莉迪亚渐生爱意，却在她被公牛撞成植物人后离她而去，仅仅因为莉迪亚的前男友表现出好似比他更深情的爱。

I know of none which are not easy to answer, but what I am trying to do now is only by a series of hints to throw some light on the narrow path of legal doctrine, and upon two pitfalls which, as it seems to me, lie perilously near to it.

我不认为有什么难以回答的问题，但我现在要做的仅仅是通过一系列线索，来给法律理论的狭窄路径以及在我看来离它近得危险的两个陷阱提供一些启示。