查询词典 lipa

It is reported that SarA regulates the transcriptions of lipA encoding lipase, zinC encoding membrane-associated zinc metalloprotease and atlE encoding autolysin. SarA exhibiting an up-regulation on lipA and zinC, and down-regulation on atlE were demonstrated by reverse-transcription PCR and reporter gene lacZ analysis respectively.

RT-PCR和lacZ报告基因的分析结果显示，在表皮葡萄球菌ATCC35984中，SarA对atlE表达起负调控作用，而对lipA和zinC（膜相关锌金属蛋白酶基因）的表达则有正调控作用。

The results demonstrate that SarA regulated directly the expressions of lipA, zinC and atlE by protein-DNA binding to the promoter region of AT-rich sequence.

生物信息学分析表明，SarA控制atlE，lipA和zinC 3种基因表达可能是通过与被调控基因上游的特定DNA序列的结合来实现的，该DNA结合区保守并富含AT碱基。

The art college next to LIPA where John Lennon and original bassist Stu Sutcliffe attended.

LIPA旁边的艺术学院，是约翰列侬和原装贝斯手史都萨克里夫上学的学校。

Under the supervision of two teachers, Michael Romano and Galit Lipa, they try to bring the most egregious injustices of California's three-strikes law before judges.

在两名老师的监督下，他们的名字是Michael Romano和Galit Lipa，他们努力把加州最严重的不公正的三犯就出局的案例提交给法官。

The lip gene has higher potential for lipid-rich food wastes treatment compared to the lipA gene.

此脂解？对C10至C12酯类及C8至C10之三甘油脂有较佳的分解活性，lip基因比lipA基因较有被应用於高油脂厨余分解油脂的应用潜力，因为在选殖这些脂解？

The HPV positivity amongst the group of women with no cervical abnormality was 43%, while that in women with cervical dysplasia and cervical cancer was 81.2% PCR products from 89 HPV positive cases were genotyped by LiPA, of which 49 cases were single HPV infected concerning 11 different HPV types and the commonest type was HPV-16, occurring in 25(51%) of all single HPV infected cases. The other 40 specimens contained multiple HPV types comprising 16 different HPV types. Although double infections(n=25) prevailed, triple(n=9),quadruple(n=3) and quintuple(n=3) infections were also found. PCR products of four HPV positive cases failed to be classified by LiPA. Sequencing analysis revealed that these 4 cases were HPV-66 which was not included in LiPA probes. Follow-up data of 12 cases confirmed the HPV genotyping results. Conclusion The LiPA for HPV genotyping is easy to perform.

结果 155例妇女子宫颈刮片中HPV DNA检出率为60%，其中在宫颈细胞学检查正常或仅有炎症病变的妇女中HPV DNA阳性率为43%，而在宫颈细胞增生不良或宫颈细胞癌的妇女其阳性率为81.2%。93例HPV阳性的标本中，89例利用LiPA成功地作出了基因分型，其中单独一型HPV感染为49例，涉及11种不同基因型HPV，最常见的型别为HPV 16，占单独感染的51%(25/49)；另40例为不同型别HPV重叠感染，涉及所有16种HPV基因型别，二重感染占主导地位(n=25)，同时也检测出其它多重感染。4例HPV阳性的标本应用LiPA未能作出基因分型，DNA序列分析显示这4例为HPV 66感染，此型别不包括在LiPA所标记的探针内。12例随诊资料显示了LiPA的物异性及高度的重复性。

Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

Assisted by DNA2.0 and Gene2Oliga software, we optimized the codon usage and secondary structure of RNA and induced enzyme sites Cla I (237 site) and Pst I (475 site) into the gene. In the first step, fragments F1 (237 bp), F2 (238 bp) and F3 (422 bp) were separately synthesized by assembly PCR. In the second step, fragments F1, F2 and F3 were separately digested by Cla I and Pst I, and then ligated into a full length lipA gene.

首先在DNA2.0和Gene2Oliga软件辅助下对lipA基因密码子及RNA二级结构进行优化并引入Cla I(237位)和Pst I(475位)酶切位点；通过组装PCR分别合成lipA基因的各片段F1(237 bp)、F2(238 bp)和F3(422 bp)；通过该基因内的Cla I和Pst I限制性酶切位点连接成完整的全长lipA基因。

The expression of cloned cold-adapted lipase gene lipA in Pichia pastoris GS115 with the pPIC9k as the expressive vector was performed according to the methods described in the manual, version 3.0, of the pPIC9K identified the lip gene cloned here was a functional lipase gene.

以pPIC9K为表达载体，以GS115为表达宿主菌，成功构建重组表达质粒pPIC9K-lipA，经甲醇诱导表达，能够分泌有脂肪酶活性的表达产物，因此证明本研究所克隆的脂肪酶基因lipA确实为脂肪酶开放阅读框。

Then, we cloned the lipA and its partner gene lipB into the vector pUCPCM and pBBR22b both or separately. Therefore, we got 7 expression plasmids pBBR22blipAB, pBBR22blipA, pUCPCMlipAB, pUCPCMlipA, pUCPCMΔlipAlipB, pUCPCMΔlipA, pUCPCMΔlipB, and then electroporated them into B.

接着把lipA和它的伴侣基因lipB单独或全部克隆到载体pUCPCM和pBBR22b上，构建出pBBR22blipAB、pBBR22blipA、pUCPCMlipAB、pUCPCMlipA、pUCPCMΔlipAlipB、pUCPCMΔlipA、pUCPCMΔlipB七种表达质粒，通过电转化将上述表达质粒转化到含T7 RNAP的B。

Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行，五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益，这将是我们的荣幸。