查询词典 knock into

Hancock: Knock, knock. Gesundheit.

敲门，敲门，身体健康

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

She is not in the washroom and then stopping, because it is finally his own point of view that he cares about and not hers, and with this firmly in mind, and a light, confident step, such as you might find in the works of Bulwer-Lytton, he enters the space she occupies so prettily and, taking her by the hand, proceeds to tear off the stiff white hospital gown (but no, we cannot have that kind of pornographic merde in this majestic and high-minded sentence, which will probably end up in the Library of Congress)(that was just something that took place inside his consciousness, as he looked at her, and since we know that consciousness is always consciousness of something, she is not entirely without responsibility in the matter) so, then, taking her by the hand, he falls into the stupendous white puree of her abyss, no, I mean rather that he asks her how long it has been since her last visit, and she says a fortnight, and he shudders, and tells her that with a condition like hers (she is an immensely popular soldier, and her troops win all their battles by pretending to be forests, the enemy discovering, at the last moment, that those trees they have eaten their lunch under have eyes and swords)(which reminds me of the performance, in 1845, of Robert-Houdin, called The Fantastic Orange Tree, wherein Robert-Houdin borrowed a lady's handkerchief, rubbed it between his hands and passed it into the center of an egg, after which he passed the egg into the center of a lemon, after which he passed the lemon into the center of an orange, then pressed the orange between his hands, making it smaller and smaller, until only a powder remained, whereupon he asked for a small potted orange tree and sprinkled the powder thereupon, upon which the tree burst into blossom, the blossoms turning into oranges, the oranges turning into butterflies, and the butterflies turning into beautiful young ladies, who then married members of the audience), a condition so damaging to real-time social intercourse of any kind, the best thing she can do is give up, and lay down her arms, and he will lie down in them, and together they will permit themselves a bit of the old slap and tickle, she wearing only her Mr.

然后阻止，因为到最后那是他自己的关心的观点而不是她的，在他的脑子里根深蒂固，他自信地走了一步，这就像你也许会在布尔沃？里顿的作品中看到的一样，他进入兴高采烈地进入她占有的空间，抓住她的手，开始撕开他白色僵直的病号衣（但是我们没有在他冠冕堂皇的充满智慧的语句中发现那种带有色情的废话，那也许会在国会图书馆中绝迹）（在他的意识中有一些东西正在被替代，当他看她的时候，就如我们知道的意识始终是意识，她在这个事情中不是完全没有责任的），所以，然后抓起她的手，他掉进了她深渊的巨大白色水中，不，我想他问她自从她上回来已经多久了，她说有两个星期了，他战栗着告诉她她现在的情况（她是一个非常受欢迎的女兵，她的部队通过埋伏在森林里赢得了所有的战斗，在最后的时刻敌人发现了，那些他们吃掉他们午餐的树有了眼睛和剑）（这使我想起了一八四五年罗伯特？乌丹的演出，叫做《奇幻橘子树》，在其中罗伯特？

She is not in the washroom and then stopping, because it is finally his own point of view that he cares about and not hers, and with this firmly in mind, and a light, confident step, such as you might find in the works of Bulwer-Lytton, he enters the space she occupies so prettily and, taking her by the hand, proceeds to tear off the stiff white hospital gown (but no, we cannot h39e that kind of pornographic merde in this majestic and high-minded sentence, which will probably end up in the Library of Congress)(that was just something that took place inside his consciousness, as he looked at her, and since we know that consciousness is always consciousness of something, she is not entirely without responsibility in the matter) so, then, taking her by the hand, he falls into the stupendous white puree of her abyss, no, I mean rather that he asks her how long it has been since her last visit, and she says a fortnight, and he shudders, and tells her that with a condition like hers (she is an immensely popular soldier, and her troops win all their battles by pretending to be forests, the enemy discovering, at the last moment, that those trees they h39e eaten their lunch under h39e eyes and swords)(which reminds me of the performance, in 1845, of Robert-Houdin, called The Fantastic Orange Tree, wherein Robert-Houdin borrowed a lady's handkerchief, rubbed it between his hands and passed it into the center of an egg, after which he passed the egg into the center of a lemon, after which he passed the lemon into the center of an orange, then pressed the orange between his hands, making it 56aller and 56aller, until only a powder remained, whereupon he asked for a 56all potted orange tree and sprinkled the powder thereupon, upon which the tree burst into blossom, the blossoms turning into oranges, the oranges turning into butterflies, and the butterflies turning into beautiful young ladies, who then married members of the audience), a condition so damaging to real-time social intercourse of any kind, the best thing she can do is give up, and lay down her arms, and he will lie down in them, and together they will permit themselves a bit of the old slap and tickle, she wearing only her Mr.

然后阻止，因为到最后那是他自己的关心的观点而不是她的，在他的脑子里根深蒂固，他自信地走了一步，这就像你也许会在布尔沃？里顿的作品中看到的一样，他进入兴高采烈地进入她占有的空间，抓住她的手，开始撕开他白色僵直的病号衣（但是我们没有在他冠冕堂皇的充满智慧的语句中发现那种带有色情的废话，那也许会在国会图书馆中绝迹）（在他的意识中有一些东西正在被替代，当他看她的时候，就如我们知道的意识始终是意识，她在这个事情中不是完全没有责任的），所以，然后抓起她的手，他掉进了她深渊的巨大白色水中，不，我想他问她自从她上回来已经多久了，她说有两个星期了，他战栗着告诉她她现在的情况（她是一个非常受欢迎的女兵，她的部队通过埋伏在森林里赢得了所有的战斗，在最后的时刻敌人发现了，那些他们吃掉他们午餐的树有了眼睛和剑）（这使我想起了一八四五年罗伯特？乌丹的演出，叫做《奇幻橘子树》，在其中罗伯特？

On the other hand, beautiful thoughts of all kinds crystallize into habits of grace and kindliness, which solidify into genial and sunny circumstances; thoughts of courage, self-reliance, and decision crystallize into manly habits, which solidify into circumstances of success, plenty, and freedom; gentle and forgiving thoughts crystallize into habits of gentleness, which solidify into protective and preservative circumstances; loving and unselfish thoughts crystallize into habits of self-forgetfulness for others, which solidify into circumstances of sure and abiding prosperity and true riches.

截然相反的是，各种美好的思想会转变成坚强的习惯，它们会促成充满欢乐的环境；勇敢，自信与果断的思想会转变成坚强的习惯，它们会巩固充满成功，富足与自由的环境；文雅，宽大的思想会转变成温和的习惯，它们能进一步推进安全的，有保障的环境；充满爱心的，慷慨的思想转变成无私的习惯，它们会形成稳定，繁荣，真正富足的环境。——摘自《痛苦是错误思想的结果》詹姆斯。艾伦

PCFK series of pairs of counter-reversible hammer crusher is a new crushing equipment, mainly by knock-on effects to broken materials, broken process in general is this: the material falling into the break in the world, imported high-speed rotation of the hammer impact face was against the entrance of the adjustable crusher tooth wear plate, for the first time broken, broken after the particles basically the same material evenly into the main break, the secondary primary crushing, crushing in the main chamber has been uniformly The material was re-kinetic energy particles from the hammer, high-speed rushed back inside the jagged cavity lining was broken, due to the special lining sawtooth-shaped structure.

PCFK系列可逆双反击锤式破碎机是一种新型破碎设备，主要是靠冲击作用来破碎物料的，破碎过程大体是这样的：落下的物料进入破世界进口后，遇到高速旋转的锤头冲击而被打击到破碎机进口处的可调耐磨齿板上，进行首次破碎，破碎后颗粒基本相同的物料均匀的进入主破碎内，进行二次主破碎，在主破碎腔内已被均匀的物料颗粒从锤头再次获得动能，高速冲向反击腔内部的锯齿形衬板而被破碎，由于衬板特殊的锯齿形结构。

When the studio glass in front of the curtain was opened, I saw the glass door of a happy face, curtain has fallen, and came gently knock on the door, then reached into a head, and then she stops to my side, but then disappeared, and this feeling has always been in control of me, like a pitch-black darkness, like, I will point the lights, quietly slipped into the room to see if he has been asleep.

当工作室玻璃门前的门帘被拉开时，我在玻璃门上看到一张欢乐的脸，门帘又落下了，传来轻轻的敲门声，随即伸进一个头来，接着她站到了我的身边，但随即又消失了，这种感觉一直控制着我，就像黑暗里一片漆黑似的，我会点上灯，悄悄地溜进房间，看看他是否已睡着了。

In heat shock protein gp96-peptide complexes induced tumor rejection model,antigen specific CD8T cells adoptively transffered into perforin knock out mice failed to proliferate and differentiate into CTLs whereas co-transfer of wide type NK cells rescued the clonal expansion and CTL differentiation of transferred CDST cells.These results suggested important immuno-modulatory roles of perforin and presumably different roles of perforin secreted by NK cells versus by CD8~+ CTLs.

例如，在热休克蛋白Gp96-肽复合体所诱导的抗肿瘤免疫应答中，穿孔素基因敲除小鼠中单独回输的野生型CD8T细胞不能产生有效的抗肿瘤免疫应答，而同时回输野生型NK细胞能够恢复CTL应答。

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

Then we put the s中秋节为什么要吃月饼 tuffing in the middle of the dough and make the dough into a ball and then we put the dough with stuffing into the mould, pressing the mould flat with hands, knock the mould slightly and the dough in the shape of moon will come out, and now is ready to put in the oven to bake.

再把馅放上去，这样包起来，然后用两手搓圆，把搓好的面团放进模子，再用手掌压平，然后在模子两面这么一敲，月饼就出来了，接下来就可以放进烤箱了。

No, it's unnecessary. I don't think I have a fever.

不，没有必要，我想我没有发烧。

We live in a culture of instant gratification.

我们生活在即时满足的文化中。