查询词典 insertional

To study the function of this ORF, we constructed Tn5gusA5 insertional and integrational mutants in the corresponding gene of wild type strain 8004 and analyzed the phenotypes of the mutants.

为了研究该σ因子在Xcc中的作用，我们构建了该基因的Tn5gusA5的插入突变体和pK18mob定点整合突变体并进行了表型分析。

The strategis of large-scale mutagenesis and gene screening include chemical mutagenesis,insertional mutagenesis and gene trap.

用于大规模基因突变与筛选的主要策略有化学诱变、插入突变、基因诱捕。

Since SouthLoc uses data of Southern blotting as the only source data and it focuses on special genome, it is very valuable not only in analysis of foreign gene\'s insertion status but also in large-scale analysis of insertional mutagenesis. Therefore, the tool has great application potential.

由于SouthLoc只需要使用Southern杂交的数据，而且其是针对某一特定基因组设计优化方案的，因此，无论是用于大规模的插入突变库的分析，还是用于验证表达菌株的外源基因插入情况，SouthLoc都可以体现出其具有的优势，具有很好的应用前景。

There was higher insertional polymorphism in mangrove populations than inland populations, probably suggesting the activation of retrotransposons by stress and environmental factors.

不同种群的核苷酸多样性水平变化很大，但总的说来，马来半岛的种群核苷酸多样性水平最高，而琉球群岛的种群核苷酸多样性水平最低。

As a result, PB-mediated insertional mutagenesis permits for the first time the efficient production of mouse mutants for systematical functional analysis of the mouse genome.

PB转座系统第一次提供了在哺乳动物基因组水平高效诱变基因的工具，有助于系统地研究基因功能。

Non-viral vectors have important safety advantages over viral approaches, including their reduced pathogenicity and capacity for insertional mutagenesis, as well as their low cost and ease of production.

对于非病毒载体的研究主要集中在靶向性、转染方式和转染效率等领域，较少关注病毒载体介导目的基因定点整合和目的基因长期表达等方面的研究。

Genomic Southern blot analysis showed that about two thirds of the transformants were single T-DNA insertional events.

该突变体不能侵入水稻叶片及擦伤的大麦或水稻叶片，说明Al-412突变体在寄主组织中扩展进程被阻断。

Insertional mutagenesis is a method for identifying genes by using the integration of DNA as the mutagen,thereby facilitating the cloning of the mutated gene.

插入突变是一种通过外源DNA整合的方式来获得突变体，并克隆得到对应突变基因的方法。

But it is also difficult to identify a mutant related to Tos17 because there are multiple copies of Tos17 in a mutant and only about 10 percent of mutants are from Tos17 insertion. Theoretically, Ac/DS two-element system is regarded as a best approach for rice insertional mutagenesis.

Tos17是目前应用最为成功的反转录转座子，但多拷贝的插入使突变体的表型鉴定和分子鉴定较为困难，因为只有10%左右的突变性状是由Tos 17插入引起的。

About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function.

利用一个具有启动子捕获和激活标签功能的载体 p CAS04，我们创制了一含25，000个独立转基因株系的T-DNA突变体库。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。