查询词典 insertion point

On the other hand,the Inverse PCR combined with a tail-to-tail primer design requires T4 DNA ligase enzyme to ligate PCR products,which makes the positive rate relatively low.We aim to find out an easy and effective method to perform gene deletion, insertion and substitution mutation.

近几年，有文献报道使用基于Quikchange原理的方法来制作DNA片段的插入、替代以及删除突变，但由于QCM-PCR的低产量，难以产生阳性克隆。

The results indicate that use of the minimally invasive nail insertion technique significantly decreases the occurrence of malalignment in proximal femoral shaft fractures.

结果显示使用微创髓内钉插入技术明显减少了股骨干近端骨折对线不良的发生率。

The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物，且所有试材的扩增结果一致，均无目的条带以外的产物扩增，说明Ty1-copia类逆转座子在苹果属植物中广泛存在，且存在历史可能较为久远；从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066)，结果表明，同一基因组来源的这些序列存在高度的异质性。

Therefore, insertion of a femoral nail with the hip in increased flexion and adduction might help to lower the risk of injuries to the superior gluteal nerve and the gluteus medius muscle.

因此，插入股骨髓内钉时，增加髋关节的屈曲内收可能降低损伤臀上神经和臀中肌的风险。

Therefore, insertion of a femoral nail with the hip in increased flexion and adduction might help to lower the risk of injuries to the superior gluteal nere and the gluteus medius muscle.

因此，插入股骨髓内钉时，增加髋关节的屈曲内收可能降低损伤臀上神经和臀中肌的风险。

The risk of injury to the superior gluteal nerve and the gluteus medius muscle during closed antegrade insertion of a femoral nail is lessened by increasing the amount of hip flexion and adduction.

在闭合顺性插入股骨髓内钉时，增加髋关节屈曲和内收的度数有利于减小损伤臀上神经和臀中肌的风险。

The risk of injury to the superior gluteal nere and the gluteus medius muscle during closed antegrade insertion of a femoral nail is lessened by increasing the amount of hip flexion and adduction.

在闭合顺性插入股骨髓内钉时，增加髋关节屈曲和内收的度数有利于减小损伤臀上神经和臀中肌的风险。

This additional flexibility may not be aailable when a cementless femoral component is used, since depth of insertion is determined more by the fit within the femoral metaphysis than by the leel of the neck osteotomy.

这种灵活性在无骨水泥固定假体时可能不存在，因为假体植入深度更多地取决于其在股骨干骺端的贴合情况而非股骨颈的截骨水平。

Methods 25 corpses were dissected. The origin, insertion, the construction of muscle bundle, and the relationship with adjacent structures of multifidus muscle were observed and measured.

对25具尸体多裂肌的起、止点、肌束的构成、与周围组织结构的关系、多裂肌短腱与止点及其与棘突和棘间韧带的关系进行解剖测量。

Results: There were 4 mutations which were insertion, replacement and non-sense muta ti on in 13 cases of VHL patients or candidates.

结果：对13例VHL患者或有家族史者检测，发现4例有突变，突变类型有碱基插入、置换和无意义变异。

They have quite a lot of work to do.

他们有许多工作要做。

Let us give you a more elaborate example of this process.

让我们给你这个过程的更多的详细例子。