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The signal peptides of chicken Igλ light chain and murine plasmingen were fused to the GFP N terminus using site-directed mutagenesis to construct the vectors with signal sequence, pEGFP-SPc and pEGFP-SPm. The chicken Igλ gene was inserted into the two vectors with signal sequence after double enzyme cutting.

分别将鸡Igλ轻链信号肽、小鼠纤溶酶原信号肽与pEGFP-C1载体的绿色荧光蛋白N端融合，产生带有信号肽的中间载体pEGFP-SPc和pEGFP-SPm。

By means of polymerase chain reactionand DNA recombinant techniques,the gene encoding mature ouse IL-4 was obtained from plasmid pCD-mIL4 and was inserted into expressionvector pBV220,which contains _RP_L double promoters,synthesized SD sequence and temperaturesensitive CIts857 gene,thus an expression lone eferred as pBV-mIL4 was constructed.

该质粒含有 P_RP_L 串联启动子，合成 SD 序列和温度敏感的CIts857基因，并且去除了完整小鼠 IL—4 cDNA 中3′侧翼非编码区，以原核系统高频使用的强终止密码子TAA 代替了原小鼠 IL-4基因的终止密码子 TAG，经 SDS-PAGE 及凝胶密度扫描分析，表达的重组小鼠 IL-4分子量约为14.2KDa，占总菌体蛋白量的25～30%。

In addition to Dopamine, Doubter and Nipride treatment, IABP was inserted on Aug.

除了给予多巴胺，多巴酚丁胺和硝普钠治疗外，r8月22H起置入主动脉弓内球囊反搏治疗。

Auto inserted technology of dowel bar is one of important technologies for long-life pavement.

传力杆自动植入技术是长寿命路面诸多关键技术的重要技术之一。

Then, two or three drainage tubes will be inserted around you liver, to drain away excess blood and fluid during the operation.

然后，两个或三个引流管将插入你周围的肝，排出过剩的血液和液体在行动中。

Today, one assumes, they will all be passing around snaps, oohing and aahing over each others' pictures of water meters and close-ups of PVC sealant inserted into draughty gaps.

可以想象，3月份上班第一天，他们一定会互相传阅照片，对着彼此的水表照片和插入通风口的聚氯乙烯密封材料大特写，不停地发出啧啧赞叹。

The drawing opens with a title block already inserted.

图面在开启时，已经插入一个标题栏框。

FT van den Ham, Leyden, 1890; Sayyid 'Ali Mohammed, Zad alil (Indian lithograph, 1290=1873; the Biblical references are inserted in the Arabic text with Hebraic letters and Arabic transcription); Proof of the Prophet Mohamet from the Bible, No. 23 of the publications of the Mohammedan Tract and Book Depot, Lahore, is wholly modern; Al-Kanz al-Maurud fi-ma Baiya 'Alaina min Nas Shari'at al-Yahud a Druse polemic against the Pentateuch; extracts from it have been published by I. Goldziher in Geiger's Jüd.

金融时报范登贝火腿，莱顿， 1890 ；赛义德阿里穆罕默德， Zad alil （印度光刻， 1290 = 1873年；圣经提到插入的阿拉伯文与希伯来文和阿拉伯文字母转录）；证明先知Mohamet从圣经，第23号出版物的穆罕默德道和图书站，拉合尔，完全是现代的；基地Kanz基地， Maurud纤维瘤Baiya ' Alaina民Nas Shari'at基地， Yahud （一德鲁西对争论五；提取它已经出版了一Goldziher在盖的珠德。

Typically, this would be inserted into a database to save a lot of data entry.+ Dynamic exchange of up-to-date info between users.

通常人们通过向数据库中插入这些数据来保存大量数据，用户之间可动态交换最新的信息。

And with reference to Figure 1, the treatment line (18) on the plug (10) inserted into the output jack (9), the other side of the two output electrodes, respectively, to insert ear plug mold, patches, foot molds and correct placement in the body or body parts protruding point on.

并参照图1，将诊疗线（18）上的插塞（10）插入输出插口（9），另一端两个输出电极插塞分别插入耳模、贴片、脚模等，并将其正确安置在人体凸出部位或体穴上。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。