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The results showed: after local leafhoppers were inoculated with RDV- SX isolate, RDV- LQ isolate, the vector viruliferous rates were similar as 84 % and 75% respectively,but the rice incidence rates were dissimilar as 28.1% and 2.8 % respectively.

研究表明：用本地黑尾叶蝉分别接种RDV本地分离物和云南分离物，斑点杂交显示介体带毒率相似，分别为84%、75%，但生物学接种结果差异相当大，分别为28.1%，3.8%，另外斑点杂交显示云南病区的黑尾叶蝉带毒率为88%，说明介体叶蝉的传毒能力具有地域性，介体叶蝉带毒率与传毒能力也存在一定差异。

Inoculating test plants through vector,observing symptom and using universal primers(R16mF2/R16mR1)for phytoplasma 16S rDNA were conducted.1.4 kb DNA fragments were amplified by polymerase chain reactionin DNA samples extracted from inoculated wheat and viruliferous Psammotettix striatus L.respectively.

采用介体接种植物，症状观察和应用植原体16S rDNA基因通用引物对R16mF2/R16mR1进行PCR扩增，在接种小麦和传毒介体中均扩增出1.4kb的特异片段，鉴定出小麦蓝矮植原体新寄主7种。

Anticoccidial index increased by 73.50, rate of weight increased by 39.19%, mortality decreased by 33.4% in chickens inoculated with E. tenella by administering vitelline antibody.

tenella鸡的死亡率降低33.34%，增重率和抗球虫指数分别提高39.19%和73.50；(3)特异性高免卵黄抗体对E。

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

Osteoblasts isolated from the cranium of Zelanian rabbit,after being transfected with green fluorescent protein,were inoculated onto the bio-derived bone to investigate the 3D fabrication of engineering bone in rotating wall vessel bioreactor.

用经绿色荧光蛋白标记的新西兰兔颅骨来源的成骨细胞复合到生物衍生骨支架材料上，在新型旋转壁式生物反应器内三维构建组织工程骨，同时与静态培养环境中构建的结果进行比较。

But in Li Zhi's understanding,"human"is inoculated withthe world,and he can not give up the Confucianism ethic and the spirit ofconcerning with the worldly affairs.

然而，在李贽的理解中，&人&是万物一体之&人&，他不能放弃儒家的伦理道德和入世情怀。

Variation of microbial communities in inoculated and natural composts was studied with the traditional incubation method and the PCR-DGGE technology.

采用传统培养方法和PCR-DGGE技术研究了人工接种堆肥和自然堆肥微生物群落的演变过程。

LA795 cells were inoculated subcutaneously into the dorsa of T739 mice, and the mice were randomized into 3 groups.

将接种LA795肺腺癌细胞的T739小鼠随机分成3组，分别给予rhES，hAS或等体积PBS皮下注射，1次／日，共14d。

In general, explants began to swell in four days after being inoculated on media.

外植体在含激素的培养基上产生愈伤组织的过程中对激素种类，用量不敏感，愈伤组织的诱导频率一般为90.0%左右。

He inoculated his computer with a population of 64,000 very simple software programs.

他预先给计算机输入645，000组非常简单的软件程序。

Cable dampener cannot be rolled up but must be hung over the cable.

缆帆不能被卷起，必须保证处于展开状态

"This skylark is a present from my master," he says.

"这只云雀是我主人献上的礼物。"他说。