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The all samples were extracted with 80% methanol and determined for contents of IAA^ ABA GA, and iPA by indirected ELISA.

结果表明:(1)在花器官中,IAA、ABA、GA_3、ZR、iPA在不育系和保持系间的变化趋势不一致,含量也有很大差异。

Methods The virus titer in culture fluid was measured by enzyme-linked immunosorbent assay.The HTNV-antigen in MsC was detected by indirected immunofluoresence assay and enzyme immunoassay.The shape and ultrastructure of infected MsC were observed by light microscope and transmission electron microscopy respectively.

采用免疫标记技术和电镜技术观察HTNV-A9株对人胚肾小球系膜细胞的感染情况和病毒唑、磷甲酸钠对病毒感染的抑制作用。

The distribution of PTA1 molecule on NK cell, NK cell lines and NK cell clone was detected by indirected immunofluorescence staining and FCM analysis. The results showed that PTA1 expression rate on activated NK cell in MLC was above 60%.

结果证明这种方法能够稳定获得NK细胞克隆,应用流式细胞仪检测获得的一株NK细胞克隆表型为 CD56CD3-。

An indirected ELISA was established with the purified recombinant protein, after the square matrix titrate determination best density is 2.43μg/mL, the blood serum best dilution ratio is 1:40,Compares with ten reagents boxes, proved we establish the ELISA method has the high specificity and the sensitivity.

以纯化的重组蛋白建立了初步的间接ELISA方法,经方阵滴定确定最佳包被浓度为2.43μg/mL,血清最佳的稀释比为1:40,与万泰试剂盒相比较,证明我们建立的ELISA方法有较高的特异性和敏感性。

In the indirected oxidation of Mn(superscript 2+), the yield of the oxidative product Mn(superscript 3+) increased by 11.11% or 18.51% respectively in the presence of Ag or Ag2O nanoparticles.

将这2种纳米粒子应用于Mn电氧化,使其转化率分别提高了11.11%和18.51%。

To investigate the interaction between heat shock protein 70(HSP70) and alpha-fetoprotein in human hepatocellular carcinoma cell line BEL-7402,the expression and localization of HSPT0 and AFP in human hepatocellular carcinoma cell line BEL-7402 were determined by immunocytochemistry and indirected immunofluorescence cytochemicai staining.

甲胎蛋白是由胚胎肝细胞、卵黄囊和胚胎消化道微量合成的主要血清蛋白[1]。

MATERIALS AND METHODS: hMSCs labeled with enhanced green fluorescent protein were transplanted into the lateral ventricle of neonatal mouse brain. At 0, 9 and 14 days post-transplantation, MICE were sacrificed and their brains were fixed with 4% paraformaldehyde. The engraftment of transplanted cells in coronal section of the recipient mouse brain was examined wider a fluorescent microscope. Indirected immunofluorescent staining was used to detect the expression of neural proteins of these grafted cells.

材料与方法:将标记绿色荧光蛋白的人骨髓间充质于细胞植入到新生3d的小鼠侧脑室中,分别于移植后0d,9d和14d处死受休鼠,取其脑组织,4%多聚甲醛固定后行冠状面冰冻切片,荧光显微镜下观察hMSCs的植入,激光共聚焦显微镜检测植入细胞神经特异性蛋白的表达,定位双重标记的植入细胞。

TSPG has the similar effect as HGFs, can directly or/and indirected effect and regulate proliferation and differentiation of CD34〓 HSC/HPC to bring into full play in"invigorating qi"of Panax ginseng. 2. During the process of the extensive proliferation, orderly differentiation, functional activation and gene transfer of hematopoietic stem/progenitor cells in ex vivo culture systems, TSPG may be used to regulate the proliferation and differentiation of hematopoietic cells to perform potential application in the domain of hematpoietic stem cell engineering.

我们的研究结果提示:1、TSPG具有类似造血生长因子的功效,能直接或间接影响和调控造血干/祖细胞的增殖与分化,促进血细胞的生成,从而发挥人参&补气生血&之功效。2、在进行对造血干/祖细胞的扩增与诱导分化,功能激活,基因转染等造血干细胞工程研究中可利用人参皂甙的药理作用来调控细胞的增殖分化,发挥其在造血干细胞工程领域的潜在应用价值。

Results showed diural variations of leaf Pn displayed bipeaked curve model, indicating the depression of photosynthesis at noon. Leaf Pn rate in June was higher than in September; Compared to leaf Pn, Cs had a similar changing curve, Cs in June was higher than in September. While diural variations of intercellular CO2 concentration were opposite to that of leaf Pn and Cs, decreased before 1200,increased after 1200, decreased again at 1600,after 1600 increased again; diural variations of climatic factors displayed single peaked curve model, peakedvalue occurred at 1400,and their influences on physiological factors were indirected and integrated.

结果表明,光合速率的日变化具有双峰型,存在光合午休现象,6月的光合速率高于9月;沙棘的气孔导度较低,其日变化与光合速率有相似的变化趋势,6月的气孔导度大于9月;细胞间CO2浓度的日变化与光合速率和气孔导度的日变化相反,1200之前下降,之后上升,1600下降,然后上升;气象因子的日变化具有单峰型,峰值1400出现,对生理因子的影响是间接且综合的。

The entropy-directed algorithm needs manual intervention to construct fault isolation strategy,and it is ofεhigh computation complexity.Theoretical analysis and experimental results illustrate that the entropy indirected algorithm remedies such deficiency. The MFIT of the strategy by this algorithm is about 38% less than that by the entropy- directed algorithm under the assumption of unequal fault rate.

实验验证和理论分析表明,该方法弥补了直接熵法构造故障隔离策略时需进行人工干预、计算复杂性高的不足;在不等故障概率的假设条件下,平均故障隔离时间比直接熵法减少约38%。

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Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行,五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益,这将是我们的荣幸。

The report also shows that the proportion of unmarried men and women living together has doubled between 1986 and 2006, with 13 per cent of those aged 16 to 59 now cohabiting.

报告还指出,从1986年至2006年,英国未婚男女同居的比例增长了一倍,在16岁至59岁的人群中,有13%的人同居。