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In this study, to define the functional roles and mode of the upstream regulatory elements ofα-globin gene cluster,αMⅡwas obtained, in which the 31.7kb sequence including HS-33, HS-10、HS-8 and HS-4 was deleted by defective prophage mediated by the homologous recombination;αMⅢwas also constructed, in which the 35.4kb sequence including HS-40、HS-33、HS-10、HS-8 and HS-4 was deleted by the method of temperature sensitive shuttle vector mediated homologous recombination.

在此基础上，为了定义α-珠蛋白基因簇上游各调控元件的调控功能，并探讨其作用机制，本研究利用缺陷型原噬菌体介导的同源重组法修饰α-BAC，删除包括HS-33、HS-10、HS-8和HS-4在内长达31.7kb的序列得到BAC突变体αMⅡ；又利用温度敏感型的穿梭载体介导的同源重组法删除包括HS-40、HS-33、HS-10、HS-8和HS-4在内达35.4kb的序列，得到BAC突变体αMⅢ。

There is no relation between the repeat sequence of the small plasmid and sequence of 16s RNA in different strains.

具有不同的重复序列的小质粒的各菌株间的16SRNA序列上的差异没有对应关系。

The data in the base touch upon the gene, RNA and protein sequence of the receptors as well as the data of multiple sequence alignments, similarities, atomic ordinates.

它包含的数据涉及受体的基因、RNA和蛋白质序列以及多序列联配、相似关系、原子坐标的数据。

Similar lamellar hybrids can be obtained from octa(y-chloroammoniumpropyl) polyhedral oligomeric silsesquioxanes dodecyl benzene sulfonic acid sodium salt C_(18H_(29)NaO_3S, DBSS. Further research shows that different POSS to surfactant ratios result in different arrangement of layers: when there exists excessive POSS, the layer is arranged in a "Alkyl-POSS-POSS-Alkyl" sequence; while on the contrary, the layer is in "Alkyl-POSS" sequence.

实验研究表明，通过调整POSS和表面活性剂的物质的量比例可以改变片层结构，发现当POSS成分大大过量的时候，片层重复单元按照&烷基-POSS-POSS-烷基&顺序排列，对应较大的层间距；当表面活性剂成分大大过量的时候，片层重复单元按照&烷基-POSS&顺序排列，对应较小的层间距。

The progress of complex structure function chart is introduced too. We study the method which we can transform a complex structure to the simple sequence structures in this paper. In addition, several examples on how to design sequence chart are also described i...

分析了复杂结构功能表图的进展情况，提出了将复杂结构转变为单序列结构的方法，并通过举例说明复杂结构功能表图的顺序图设计。

Mitochondrial cytochrome b sequence were used to study the molecular evolution and phylogeny of Anser cygnoides(15 breed of Chinese goose), Anser anser(2 breed of domestic European goose) and Anser albifrons(the lesser white-fronted goose, Genbank accession number AF363031). Sequence analysis revealed that there were 29 variable sites and 4 haplotypes in 45 sequences, nucleotide diversity and haplotype diversity were 0.0068,0.45 respectively, insertion/deletion or frameshift mutation were not found in Cyt-b gene sequences(1143bp).

本实验测定了中国家鹅15个品种、欧洲鹅2个品种共44个个体的细胞色素b基因全序列，与Genbank库中自额雁细胞色素b基因序列合并在一起比对分析，17个家鹅品种和白额雁细胞色素b基因全序列均为1143bp，序列中没有缺失、插入或移码突变，共检测到29个变异位点、4种单倍型，核苷酸多样度和单倍型多样度分别为0.00648和0.45。

In our former study, we cataloged genes expressed in human brain (about 10,000 gnes), and sequenced the orthologous sequence of these genes in chimpazee. In this study, we selected 16 of these positive-selection genes and then using PCR and Sequencing technique we acquired orthologous sequence in another two representative primates: white-brown ape and macaca, and then took further evolutionary analysis.

本文从目前已知在人的大脑中表达的基因中，选出16 个在人和黑猩猩的比较中明显受到正向选择的基因，利用PCR扩增技术和基因序列测定技术，获得了另外两个灵长类代表动物小猿、猕猴的直系同源序列，并进行了进化分析。

The invention provides a human McAb to tetanus toxin, being a human antibody, which consists of an antibody variable region and an antibody constant region or not, having the ability of neutralizing the tetanus toxin, with the variable region gene and protein sequence indicated as the sequence table 1. The invention is characterized in that the antibody can not induce obvious allergic reaction, has higher titer and longer effect without any animal virus pollution, and can be produced in an unlimited quantity, which also provides the relating biological functions, the testing methods, the manufacturing method and the application.

A 本发明提供一种人源抗破伤风毒素单克隆抗体，其为人源抗体，包括抗体可变区，该抗体包括或不包括抗体的恒定区，该抗体具有中和破伤风毒素的能力，抗体可变区的基因和蛋白质序列如序列表1所示；其具有不会诱发明显的过敏反应，有较高的效价且长效，产品无动物病毒污染，可以无限量地生产；本发明还提供了其生物功能、测定方法、生产制造方法及其应用。

Previously, short echo time STEAM sequence and short TE PRESS sequence have been used to quantify brain Cr concentration.

综上所述，本研究旨在通过动物实验，以长TE的PRESS序列外标准方法及LCModel软件定量检测活体小猪的脑Cr浓度，然后用高效液相色谱方法分析相应离体脑匀浆液的Cr浓度，比较两种方法检测结果的相关性，以此探讨活体~1H-MRS方法检测结果的准确性。

Expressed sequence tags are short, randomly selected single-pass nucleotide sequence reads derived from cDNA libraries and represent a small part of a gene.

表达序列标签(expressed sequence tags， EST)是从随机选择的cDNA 克隆进行单向测序获得的短的cDNA序列，代表一个完整基因的一部分。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。