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When chymotrypsin was immobilized inthe gels, the stability of the immobilized enzyme is much raised when compared withthe free enzyme.

当把糜蛋白酶固定到这些水凝胶中时,与自由酶比较,固定化酶的稳定性有了很大提高,而且由于水凝胶的温度敏感特性,酶的活力在30℃和4O℃表现为"开"或"关"。

The effects of water content and the added amount of triethylamine on peptide synthesis by immobilized enzyme were different from that of free enzyme. The catalytic efficiency of immobilized enzyme was better than that of free enzyme in most cases. The reuse times of immobilized α-chymotrypsin on MCM-22 are about 4-5 times.

结果表明,有机溶剂中含水量、三乙胺的加入量对自由酶与固载化酶的影响程度不同;多数情况下固载化酶的催化效果优于自由酶;吸附固载化酶的重复使用次数有限。

A kinetic model is proposed for producing invert sugar from sucrose by immobilized yeast cells and its application in batch fermentation is presented in detail. A solution of the kinetics differential equation is given to describe the effect of fermentation time, stirring speed, immobilized cells size and substrate concentration on the inversion coefficient.

提出了固定化酵母生产转化糖动力学模型,研究了该模型在批发酵过程中的应用,由给出的动力学微分方程的解表明了转化率与反应时间、搅拌速率、固定化细胞媒体大小和基质浓度间关系。

A selective and mild PS-DIB/ionic-liquid immobilized 2, 2, 6, 6-tetramethyl-piperidin-4-ol-N-oxyl catalytic oxidation procedure of alcohols to the corresponding aldehydes and ketones in ionic liquid BF4 at room temperature was developed. The oxidant PS-DIB was prepared via the iodation and acetylation of polystyrene resin. For the preparation of the ionic-liquid immobilized TeMPO catalyst, 4-(2, 2, 6, 6-Tetramethyl-1-oxyl-4-piperidoxyl)butyl bromide was first synthesized from TeMPO and 1, 4-dibromobutane.

将聚苯乙烯树脂先碘化再乙酰基化,制得了负载型二乙酰氧碘苯作为氧化剂。2, 2, 6, 6-四甲基-N-氧自由基哌啶醇与1, 4-二溴丁烷反应生成4-溴丁氧基-2, 2, 6, 6-四甲基-哌啶-1-氧化物,再与N-甲基咪唑发生季铵化反应,生成的溴化季铵盐与四氟硼酸钠进行离子交换制得氟硼酸型2, 2, 6, 6-四甲基-N-氧自由基哌啶负载离子液体。

A selective and mild PS-DIB/ionic-liquid immobilized TEMPO catalytic oxidation procedure of alcohols to the corresponding aldehydes and ketones in ionic liquid BF4 at room temperature was developed. The oxidant PS-DIB was prepared via Iodation and acetylation of polystyrene resin. For the preparation of the ionic-liquid immobilized TEMPO catalyst, 4-(2,2,6,6-Tetramethyl-1-oxyl-4-piperidoxyl)butyl bromide was first synthesized from 2,2,6,6-tetramethyl-piperidin-4-ol-N-oxyl and 1,4-dibromobutane.

将聚苯乙烯树脂先碘化再乙酰基化,制得了负载型二乙酰氧碘苯(diacetoxyiodo polystyrene,PS-DIB)作为氧化剂。2,2,6,6-四甲基-N-氧自由基哌啶醇(2,2,6,6-tetramethyl-piperidin-4-ol-N-oxyl,TEMPO)与1,4-二溴丁烷反应生成4-溴丁氧基-2,2,6,6-四甲基-哌啶-1-氧化物,再与N-甲基咪唑发生季铵化反应,生成的溴化季铵盐与四氟硼酸钠进行离子交换制得氟硼酸型2,2,6,6-四甲基-N-氧自由基哌啶负载离子液体TEMPO-IL。

Continuous culture of immobilized Acidithiobacillus ferrooxidans can be used to remove H2S and SO2. In order to reduce the negative effect of jarosite which formed during the oxidation of Fe(superscript 2+) by immobilized cells, the changing of initial pH and the ingredient in 9K medium were tried.

该固定化细胞的连续培养技术可以用于处理H2S、SO2,为了减少固定化细胞培养过程中带来许多不利效应的黄铁矾沉淀[NH4Fe3(SO4)26],采取了改变初始pH值和目前普遍采用的9K培养基中的(NH4)2SO4浓度,K2HPO4浓度三种方法。

Continuous culture of immobilized Acidithiobacillus ferrooxidans can be used to remove H2S and SO2. In order to reduce the negative effect of jarosite which formed during the oxidation of Fe2+ by immobilized cells, the changing of initial pH and the ingredient in 9K medium were tried.

该固定化细胞的连续培养技术可以用于处理H2S、SO2,为了减少减少固定化细胞培养过程中带来许多不利效应的黄铁矾沉淀 NH4Fe3(SO426),采取了改变初始pH值和目前普遍采用的9K培养基中的(NH4)2SO4浓度,K2HPO4浓度三种方法。

NMB showed an electrostaticbinding with the immobilized ssDNA, and both electrostatic and intercalative bindingswith the immobilized dsDNA.

NMB与ssDNA主要以静电作用相结合,而NMB与dsDNA通过静电和嵌插两种作用方式相合。

Carambola, after zymolysis,juicing and compositions regulation, was used for the contrast experiment of immobilized fermentation and free fermentation, the contrast experiment of immobilized fermentation under different temperature, and the optimized experiment of immobilized fermentation by multiple yeasts, respectively.

杨桃进行酶解取汁、成分调整后,分别进行固定化和游离发酵对比试验、不同温度下固定化发酵对比试验、复合酵母菌种固定化发酵优化试验。

In addition, under the above-selected nitrosification condition, the immobilized cells N4 and B08 were used for the combined short-cut biodenitriflcation with the ratio of immobilized cells 1:1 and the seed volume 15%, 27h the denitrogenation efficiency reached 97.53%; the immobilized cells N4 and B08 were used to do denitrogenation application test on eutrophic lake water of our school, 12h the NH4+-N removal rate was 84.06%.

在以上所选亚硝化条件下,按1:1的比例,亚硝化细菌N_4和反硝化细菌B_(08)固定化细胞接种量为15%,广西大学硕士学位论文固定化微生物捷径生物脱氮的研究作联合捷径脱氮试验,27h脱氮率为97.53%;并作本校富营养化湖水的脱氮应用试验,处理12h,NH4十一N去除率为84.06%。

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