查询词典 human

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

In lung cancer tissues, benign phymatoid lesions, and adjacent non cancer lung tissues, telomerase activity was determined by telomeric repeat amplification protocol assay, and the expression of human telomerase RNA component, human telomerase reverse transcriptase, and human telomerase associated protein(hTEP1) mRNA were detected with reverse transcriptase PCR. The results were compared with the histological types, degree of differentiation, and TNM stage of lung cancer.

用 TRAP法检测端粒酶活性，用逆转录 PCR法检测肺癌组织、肺部良性瘤样病变和病灶旁非癌肺组织的 RNA成分（ human telomerase RNA component，hTR）、端粒酶逆转录酶（ human telomerase reverse transcriptase， hTERT）、端粒酶相关蛋白 1（ human telomerase associated protein， hTEP1）各亚基的 mRNA表达，其结果与肺癌的组织类型、分化程度及 TNM分期进行了比较。

It is found that the estimating indexes of heart rate variability of MRR, RMSSD and HRVI etc have different sexual sensitivity. The constant temperature airflow of the simulating natural winds can increase the active level of human's pneumogastric nerves, making human body be flabby, relaxed and comfortable. The result indicates the physiological mechanism of the effect of different winding characteristics on the human thermal comfort.

研究发现MRR、RMSSD和HRVI等心率变异评价指标具有不同的性别敏感性；模拟自然风形式的等温气流吹风作用可提高人体副交感神经的活动水平使人体趋向于松弛、放松、舒适和休息状态，研究结果初步揭示了不同风特性对人体热舒适影响的生理机制。

The prehistorical sculpture-a woman touching herself on her breasts and belly shows that our prohistorical ancestors' idea of sculpture was closely associated with the instinctive human nature;The encient Egyption human sculptures have hardly ever changed,and remained the same for almost 3000 years,which reflects that the encient people believed their belief would last as long as forever,and the distinctive taste of their works;The Buddist sculptures are the tool for the religion to express it's idea. While,the encient Geece artist were seeking the hamoney and balance of human movement,which can be found easily in their works.

从史前女体雕刻的姿势多为抚腹或抱胸可以看出原始人雕塑的观念直接与生命的本能联系在一起；古埃及漫长的三千年历史中人像雕刻的姿势都没有明显变化，反映了当时人们坚持自己的信仰永恒的态度，及其艺术法则的独特风格；佛教造像把人物姿势做为传达教义的解说词；古希腊的人物雕塑的姿势体现了他们对和谐。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

Modern philosophy anthropologist Rander is graceful:"the human was the cultural existence, the human has created the culture, similarly the culture was also creating the human".

现代哲学人类学家兰德曼：&人是文化的存在物，人创造了文化，同样文化也创造着人&。

Human infection with swine influenza virus is underrecognized in Canada, and because viral strains could adapt or reassort into a form that results in efficient human-to-human transmission, routine surveillance of swine workers should be considered as part of pandemic influenza preparedness.

在加拿大，人感染猪流感尚未获得足够认识，因为病毒可以通过适应或重排形成足以引起人间传播的毒株，所以应考虑将猪场员工的常规监测纳入应对流感大流行的准备工作中。

In the end, this paper establishes the human resource stratigem for CR8EG according to the demand of its future development stratagem, expatiates the implementing cource of the human resource stratagem, including of organization structure redesignation, employee education mechanism foudation, reward and performance management amending, and the discussion about problem in actualization course of the human resource stratagem.

最后，在中铁八局集团人力资源内部和外部环境分析的基础上，结合集团未来发展战略的要求，制定了中铁八局集团人力资源战略，阐述人力资源战略的实施过程，包括组织结构重新设计、建立员工培训机制、薪酬管理与绩效管理制度的调整，并对战略的实施和发展存在的几个问题进行了探讨。

Methods: The three lung cancer cell lines-A549(highly invasive human lung adenocarcinoma cell ), 95-D(highly metastatic human lung carcinoma cell), and SPC-A-1(lowly metastatic human lung adenocarcinoma cell) were cultured conventionally in vitro. When the cells covered the bottom of culture flask, we abstracted the protein with lysis solution containing RIPA and PMSF, and obtained RNA with RNeasy Mini Kit.

将三株肺癌细胞：高侵袭的肺腺癌细胞(A549)、高转移的肺癌细胞(95-D)、低转移的肺腺癌细胞(SPC-A-1)做常规细胞培养，待细胞长满瓶底后，用RIPA和PMSF组成的细胞裂解液提取细胞蛋白质，RNeasy Mini Ki提取RNA。

The world of exercise gyms and fitness centres seems to me a sad and solipsistic and ultimately inhuman one, because the human needs commerce with the non-human, natural world to feel human.

在我看来，健身馆和美体中心是一个可悲而又唯我的世界。说到底，它是一种违逆人性的场所。因为，人类需要同身外的自然世界进行交流，这样才可以感觉到自己的人性。

These are places without aristocratic baggage; egalitarian places open to talent, self-improving, engaged in learning and innovation through networks that were at once competitive and cooperative.

这些地方没有贵族遗风作祟，而且对于那些有天分的人是开放的平等之地，这些人善于通过那些曾经很具有竞争力与合作精神的关系网进行自我提高以及学习创新。

Christine: You don't want to see me?

你不想见我？