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The colony of embryonic stem cell separated from blastula could gain five strains of embryonic stem cell and go down to posterity F7 in vitro.

从培养的囊胚中分离获得的类胚胎干细胞集落，经传代培养获得了5株胚胎干细胞株，在体外可传代培养到7代，冷冻保存复苏后，干细胞仍能存活、增殖。

AIM:Studies on totipotential stem cells using primordial germ cells have the same application prospect as embryonic stem cells, especially for the animals difficult to isolate embryonic stem cells from blastula.

目的：应用原始生殖细胞进行全能干细胞的研究具有与胚胎干细胞同样广阔的前景，尤其适合于难以从囊胚内细胞团中分离培养胚胎干细胞的动物。

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

Embryonic germ cell lines were isolated and cloned from gonad,gonadal ridge,analogue or the surrounding tissues of 9.5～13.5 days postcoitum mouse fetuses.Primordial germ cells were co-cultured with mouse embryonic fibroblasts obtained from the same conceptus.

自交配后9.5～13.5 d小鼠胎儿生殖嵴/腺或其类似物及周围组织，采用与其同源胎儿成纤维细胞共培养的方式分离克隆到具小鼠ES细胞诸多特征的胚胎生殖细胞系。

Morphologically, embryonic stem cells developed from unicells into compact three-embryonic layer embryoid body and into incompact cell population.

胚胎干细胞在形态学上由单细胞发育为致密的包含3胚层结构的拟胚体，再继续分化为松散的细胞群落。

The embryonic vessels failed to invade into the labyrinthine layer of placenta, which impaired the embryonic-maternal vascular connection. These defects could not be rescued by wild-type tetraploid blastocysts, excluding the possibility that they were due to the extraembryonic tissues.

组织免疫组化分析结果显示：毛细血管腔异常扩张，排列紊乱，不能形成大血管及细致有序的血管网络；胚外中胚层来源的卵黄囊脏层扁平上皮细胞形态变圆，与内皮细胞连接受损；胚胎血管不能侵入胎盘迷路层，胚体与母体血管连接受损。

Here, we report that SSEA-4, an early embryonic glycolipid antigen commonly used as a marker for undifferentiated pluripotent human embryonic stem cells and cleaage to blastocyst stage embryos, also identifies the adult mesenchymal stem cell population.

SSEA是在未分化的人胚胎干细胞表面早期表达，在胚泡期被剪切的一种糖脂类抗原，作者发现，SSEA也可以用来鉴定间充质干细胞。

To investigate the method of RNA isolation from human embryonic tissues and the factors influencing the quality of RNA, the RNA from human embryonic tissues obtained with drug-induced labor or non-drug induced labor were isolated by using grind with liquid nitrogen or homogenizer without liquid nitrogen.

为探讨人类胚胎组织完整RNA的分离方法及其影响因素，采用液氮研磨及匀浆器两种制备组织匀浆方法，对不同引产方式的人类胚胎组织进行总RNA分离，并对RNA质量进行鉴定。

After two hours of the mice leaveing warming boxes, 5 mice randomly selected from each group per timesection were executed by cervical vertebra luxation. The HSP70 of embryonic brain, protein content and deformations of embryonic nervous systems were quantitatively analyzed with the Western dot blot and Lowry method.

移出温箱2h后，每组每时段随机取5只脱颈法处死，采用Western dot blot 和lowry方法定量分析胚胎脑HSP70和蛋白质含量与胚胎神经系统畸形。

The results showed that water content decreased first, then increased in the gastrula stage of embryonic development, reached the highest content at the second phrase larvae stage and then began to decrease from the third phase larva stage. The content of lipid in the dry weight increased with the embryonic development stages, but decreased at intramembranous nauplius stage, the second phase larva, and the infant stage of larvae development. The rate of lipid in wet weight decreased and the content of C20:4w6、20:5w3 and C22: 6w3 decreased.

结果显示，黑斑口虾蛄在胚胎和幼体的发育过程中，水的体积分数先降低，至原肠胚期开始升高，到第二相幼体期达到最高，之后又有所回落；脂类占组织干重的比率呈升高趋势，但分别在膜内无节幼体、第二相幼体和仔虾蛄期略有降低；脂类占组织湿重的比率呈降低趋势；花生四烯酸、EPA和DHA的体积分数都呈降低趋势。

World War II: Battle of Saipan - Americans take Saipan.

1944年的今天，美国在塞班战役中拿下塞班岛。

When the scorner, the rejecter of truth, has become presumptuous; when the routine of work in the various money-making lines is carried on without regard to principle; when the student is eagerly seeking knowledge of everything but his Bible, Christ comes as a thief.{DA 635.3

当讥诮和拒绝真理的人，到了任意妄为时，当追求财利者罔顾按道义经营谋利时，当学人正在兢兢业业地研究各种知识，而单把圣经丢在一边时，基督要像贼一样来临。