查询词典 embryogenic

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis × L . princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

It shows that the embryogenic tissues are composed of a group of small meristematic cells at one end (embryo-proper cells), which are at different phages of cell division and many long cells at the other end (embryo-suspensor cells).

研究表明，胚性愈伤组织是由不同发育阶段的早期胚组成的细胞团，早期胚主要由长的胚柄细胞及其顶端的胚头细胞组成，而后者又具不同的分裂相。

The ECSs from embryogenic calli were higher in quality than those from meristematic globules.

香蕉的基因型和品种不同，从胚性愈伤组织或分生小球体建立ECS的可能性不同。

In the favorable inoculation season, the induction percentages of meristematic globules from flowers and embryogenic calli from scalps were 15～80％ and 0～10％ respectively, depending on the cultivars and incubation conditions.

从多芽体获得胚性愈伤组织的概率大约为0～10％，胚性愈伤组织的诱导率受基因型和品种、接种时期及培养条件等因素的影响。

As explants for the establishing of ECSs, meristematic globules underwent a process completely different from embryogenic calli.

从分生小球体起始建立ECS，其发育途径与以胚性愈伤组织的截然不同。

In this experiment, eight varieties, seven of which belong to species Onobrychis viciaefolia Scop, and one to O. t〓anscaucociea Grosh, have been studied. As a result, embryogenic calli have been obtained from tissue culture of the eight vatie- ties, and four of them have regenerated plantiets.

本实验对8个品种的红豆草（其中7个为Onobrychis vicia〓folia Scop，1个为O.transcaucosia Grosh）进行了组织培养，均得到了胚性愈伤组织，并得到了4个品种(O.viciaefolia)的再生植株。

The results showed that 7-day-precultured embryogenic calli had the best responses for bombardment among 2-7 day' preculture duration. Enhanced transformation efficiency was found in the explants treated with high osmotic medium 4h prior to till 24 h after bombardment. Particles of gold and tungsten were both found suitable for rice transformation and there was no adverse effect when tungsten particles were reduced to 1/3 standard amount for DNA precipitation on transient GUS gene expression. However, it was found that gold particle had higher GUS expression at 120 h post-bombardment. A preliminary microprojectile transformation system with an average of 15-20 blue spots/explant GUS expression was routinely obtained for 'Tainung 67' by using the particle gun with 1,100 psi rupture disk, 6 or 9 cm microcarrier flight distance and 4 mm of macrocarrier flight distance setting.

初步结果显示，以预培养2~7日之胚性癒合组织逐日测试转殖率，以预培养7日之培植体表现最佳；培植体於转殖前4小时至转殖后24小时以高渗透压培养基处理，有助於转殖效率之提高；比较金、钨粒子对转殖率在转殖后48h並无不同，但转殖后120 h取样，则金粒子组有较高之蓝点数；钨粒子用量可减少至1/3標准用量对转殖率並无影响；利用基因鎗在使用1， 100 psi裂碟片、大载片至挡片之距离为4 mm、微载髁至射击目標之飞行距离6或9 cm之设定，GUS基因表现之平均蓝点数均可达15~20个／培植体。

When being transferred to the differentiation medium, IAA and GA〓 significantly decreased, and ABA obviously increased and reached the maximum of ABA/CTK and ABA/IAA, which would help express the embryogenic capability and form multi-cellular proembryo.

具有不同胚性能力的材料之间，随着胚性能力的下降，其内源CTK、IAA和ABA水平也下降，GA〓则升高。

The embryogenic cell first divided into multicellular proembryos,then globular embryoid,pearshaped embryoid,long embryoid,scutiform embryoid and mature embryoid.

胚性细胞继续分裂形成多细胞原胚，经球形胚、梨形胚、长形胚、子叶分化期到成熟胚，其发生的胚状体与单子叶合子胚形成具有相似的过程。

Among different organ explants from ginger lily, only sheaths and leaves cultured in VW medium containing 5mg/l 2, 4-D、0.5mg/l BA、3% sucrose and 200ml/l coconut water for 3 months could be induced to form embryogenic callus. It could produce lots of germinated or ungerminated somatic embryos per culture directly from the surface of calli after culture in MS medium containing 4mg/l BA and 0.05mg/l NAA. The shoots could develop into intact plants with vigorous appearance after 2 months in culture.

叶鞘及叶培植体在含5 mg/l 2，4-D，0.5 mg/1 BA、3%蔗糖及200 ml/1椰子水的VW固体培养基中，经3个月，可诱导产生具体胚分化能力之愈合组织；并於含4 ms/l BA及0.05 ms/l NAA之MS培养基中，直接由此愈合组织的表面产生数个至数十个数目不等之体胚，2个月后可形成一完整植株。

Warden: That's a beautiful dog, but don't bring her again.

那是条漂亮的狗，但是不要再把她带来了。

You get up in the morning – no hangover!

你第二天早上起来的时候—不会宿醉！