查询词典 easy

The P32 gene of capripoxvirus were amplified by PCR.The product of PCR was cloned to the plasmid pGEM-T-Easy.Then the P32 gene was disparted to three parts.Three parts of P32 gene were inserted into the expressed plasmids pGEX-4T-1, which were identified by PCR and restriction enzyme analysis.

本研究利用PCR方法对羊痘病毒P32蛋白基因扩增，将回收PCR产物克隆到pGEM-T-Easy载体中，然后将P32蛋白基因分成3段，分别插入原核表达载体pGEX-4T-1中，经PCR和酶切鉴定，证明成功构建了重组表达载体pGEX-4T-1-N2/3、pGEX-4T-1-N1/3、pGEX-4T-1-M。

Unfortunately, I'm much sloppier than he expected---this super easy care plant still died very soon…I began to wonder whether I should have a child in the future since then

但更绝的还是我，没过多久就愣把这超 easy care 的植物给养死了，原因不祥

Another expression is "as easy as shooting fish in a barrel".

还有另外一个成语&as easy as shooting fish in a barrel&（像在木桶里杀鱼那样容易）。

This company produces the "EASY FILE" 100 licensing round, stainless steel grinding, diamond films, such as mill products, the indicators are similar products in the domestic leading position.

本公司生产的&EASY FILE&牌百页轮、不锈钢磨片、金刚石水磨片等系列产品，各项指标均处于国内同类产品的领先地位。

RAK says that advanced technologies such as Easy Oxide Mixing, Roll Feed, MDR, Double Charge and Dual Charge will make this factory one of the most advanced gres porcellanato factories in the world.

RAK公司称，其拥有的先进的技术，如Easy Oxide Mixing、Roll Feed、MDR、Double Charge和Dual Charge等，将使这家工厂成为全球最先进的gres porcellanato生产厂之一。

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

We go to great lengths to make those patches widely available, in an easy to find format, so that they will be useful to upstreams, and other distributions.

We go to great lengths to make those patches widely available， in an easy to find format， so that they will be useful to upstreams ，和其他发行版。

The reason why I send you this easy A4 copy about a lonely wayfaver's travelog but not word.doc is to persuade you to read those words under a sunshine afternoon with me, quietly and with pleasure.

专门给你寄去这份很easy的copy，是因为我真的很想让你把它拿在手上，让你坐在你办公室后的花园里，静静地阅读着(而不可以在我给你从网上发去word文档一眼就都看完了==)。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

The Globetrotters win this episode, which is fine with me, especially when Big Easy reveals that his father died two days before the race began, and he wanted to win "for his dad".

花式篮球队赢了这个赛段，还可以吧，尤其Big Easy透漏他的父亲在他参赛之前两天去世了，他是为了他父亲而赢的。

The physiology of the skin machine body for the sake of the environment in the maintenance stability, but eject a body metabolism creation of metabolism thing BE'grease', summer perspire many various skin disease light or good, autumn perspire little metabolism thing metabolism don't go out, various skin disease made, this be the skin machine body of various burning disease.

皮肤机体的生理为了维持内环境稳定，而排出身体代谢产生的代谢物是'油泥'，夏天出汗多各种皮肤病就轻或好了，秋天出汗少代谢物代谢不出去，各种皮肤病就犯了，这就是皮肤机体的各种炎症。

If I had anything tender in me, I shot it dead.

如果我有什么招标的话，我枪死亡。