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Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子,用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库,ELISA鉴定阳性克隆,DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列;免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

Seven of them were done in the presence of 3 kg hip traction, and six were in 5 kg hip traction (4 cases after 3 kg traction). Angiography was also performed in a intorted position without traction in 5 patients. In 3 cases, retinacular artery angiography was done simultaneously using the microcatheter.

造影在下列条件下完成,(1)中立位无牵引(9例);(2)中立位3kg牵引(7例);(3)中立位5kg牵引(6例,其中4例先行3kg牵引);(4)伸直内旋位无牵引状态下(5例),3例用微导管同时作了支持带动脉造影。8例作了正常髋关节中立位无牵引状态下血管造影,其中2例加做下肢伸直内旋位时造影。

The femoral circumflex arteriography of the normal leg was done in 8 patients, and three of them were simultaneously done in a postion of internal rotating of straight leg. Results Comparison with the arteriographic findings in absence of traction, all retinacular arteries but inferior arteries were depicted few branches and less femoral head perfusion in all 9 patients, especially in that of medial femoral circumflex arteriography, as hip joint was tractating in neutral position with the same speed and volume of injecting contrast medium and, the veins were demonstrated poorly or delayed.

结果同一血管部位,同等对比剂注射流率、总量造影,与中立位无牵引状态下造影所见比较,9例患髋中立位牵引时,除外下支持带动脉,所有未损伤的支持带动脉均有不同程度闭塞,相应股骨头颈部灌注不佳或几乎无灌注,静脉显影延迟或显示不清,4例牵引力由3kg增至5kg时,上述改变更为明显;5例患髋维持伸直内旋位时,所有支持带动脉尤其细小分支未见明确显示,股骨头灌注差或未见明确灌注,改变甚于牵引时;2例正常髋内旋位时造影改变类似。

He made out that he was the only real horrorshow prestoopnick in the whole zoo, going on that he'd done this and done the other and killed ten rozzes with one crack of his rooker and all that cal.

他想让我们知道他是这整座动物园里唯一一个真正的罪犯。他不停地炫耀自己曾经做过什么事,又做过什么事,甚至一拳打死十个警察,等等等等。

Seventhly, you should think about something you have done, don't think something you haven't done.

第七,经常想想自己做过的事情,不要想没做过的事情。

You shouldnt have done this. I just did what I should have done.

你不用这样,我只是做了分内该做的事情。

When I got to where I found the boy I see I couldn't cut the bullet out without some help, and he warn't in no condition for me to leave to go and get help; and he got a little worse and a little worse, and after a long time he went out of his head, and wouldn't let me come a-nigh him any more, and said if I chalked his raft he'd kill me, and no end of wild foolishness like that, and I see I couldn't do anything at all with him; so I says, I got to have HELP somehow; and the minute I says it out crawls this nigger from somewheres and says he'll help, and he done it, too, and done it very well.

我一到那个孩子所在的地方,发现非有一个助手不可,不然,我就无法把子弹取出来。按当时的情况,我无法离开,到别处去找个帮手。病人的病情越来越糟。又过了一段时间,他神志不清了,又不允许我靠近他身边。要是我用粉笔给木筏子上写下记号,他就要杀死我。他这类傻事几乎没有个完,我简直给弄得束手无策。所以我对自个儿说,我非得有个助手不可,怎么说也非有不可。

When I got to where I found the boy I see I couldn't cut the bullet out without some help, and he warn't in no condition for me to leave to go and get help; and he got a little worse and a little worse, and after a long time he went out of his head, and wouldn't let me come a-nigh him any more, and said if I chalked his raft he'd kill me, and no end of wild foolishness like that, and I see I couldn't do anything at all with him; so I says, I got to have HELP somehow; and the minute I says it out crawls this nigger from somewheres and says he'll help, and he done it, too, and done it very well.

他这类傻事几乎没有个完,我简直给弄得束手无策。所以我对自个儿说,我非得有个助手不可,怎么说也非有不可。我这么刚一说,这个黑奴不知从什么地方爬了出来,说他愿帮忙。他就这么做了个助手,而且做得非常出色。当然我断定他准是个逃亡黑奴。我实在处境为难!可是我不得不钉住在那儿,整整一个白天,又整整一个夜晚;我对你们说吧,我当时实在左右为难!

There may be a ton of reasons why it's not done but the one reason it should be done on a regular basis is that it can be a timesaver, possibly a business saver if your computer system is corrupted for any reason.

另外可能有一吨理由的原因之一,但不是做应该做的是,它有定期可以timesaver,如果你的电脑程式可能是一个商业体系败坏任何原因。

There may be a ton of reasons why it's not done but the one reason it should be done on a regular basis is that it can be a timesaver, possibly a business saver if your computer system is corrupted for any reason.

另外可能有一吨理由的原因之一,但不是做应该做定期在于它可以timesaver,如果你的电脑程式可能是一个商业体系败坏任何原因。

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This paper designs the communication system of radio telescope FAST.

针对500m口径球面射电望远镜FAST分布式控制系统的特点完成了其通信系统的设计。

Political sheep's step-by-step a large number of fossils unearthed in the county soil Ma Ali after rural village in the middle of sallow pelitic siltstone.

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如果您以高剂量快速开始,您会有如同摄入高纤维饮食似的肠胃气胀;但是,如果您以低剂量缓慢开始,并且缓慢滴定,您将可避免绝大多数剂量依赖性症状。