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The transform of phenotype of VSMC: The mRNA expressions of SM22α-a notate gene of contractile VSM-were all detected on normal aortas,but decreased normally when the aortas were destroyed and cultured in vitro for 10 days,especially in the serum groups,whose SM22αmRNA could not be detected by RT-PCR.Here,BQ123 can increased their expressions.This demonstrated that serum culture and the secretion of ET-1 can produce the transform of phenotype from contractile to synthesize type.
血管平滑肌表型的转化:RT-PCR检测发现,血管平滑肌收缩表型标志基因SM22α在正常血管平滑肌中都有表达,但在内皮损伤后体外培养10天的血管表达明显减弱,其中血清培养组血管基本不表达,加内皮素受体阻断剂BQ123后,各组SM22α的表达均有所上调,这表明内皮素分泌和血清培养促进了血管平滑肌的表型由收缩型向分泌型的转化。
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According to the feature, the blurred edge of the target in the superposition region can be detected, and the target edge parallel to the motion blur direction is detected by a Canny operator.
基于该灰度变化特征检测运动模糊方向上的目标边缘,并利用Canny算子检测平行于模糊方向的边缘,两部分边缘进行求或运算并进行形态学处理,从而分割得到完整的目标模糊图像。
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Results Expression of GFP was not detected in control group, and was detected in peritoneum of transfection group at all time points.
结果 正常对照组的小鼠腹膜组织未能检测到GFP表达,而PAMAM G9/pHK-shRNA转染组的各时间点均可检测到GFP表达,以48h表达最强,显著高于其他各时间点(P<0.01)。
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Methods: Human leukemia cell line K562 was treated with different concentrations of WM. The proliferation of K562 cells was examined by MTT assay. DNA damage in K562 cells was examined by single cell gel electrophoresis assay, and apoptosis of K562 cells was detected by Annexin V-FITC/PI double staining. The expressions of total Akt, phosphorate Akt, and NF-κB p65 mRNA and protein were detected by RT-PCR and Western blotting, respectively.
以不同浓度的渥曼青霉素作用于人类髓细胞白血病细胞K562,采用MTT法检测细胞增殖活性,单细胞凝胶电泳技术检测细胞DNA损伤形成的"彗星"样拖尾现象,Annexin V FITC/PI双标法检测细胞凋亡,Western blotting、RT-PCR检测渥曼青霉素作用K562细胞后总Akt和磷酸化Akt以及NF-κB基因及蛋白表达水平的变化。
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The PCR products were detected by both agarose gel electrophoresis and capillary electrophoresis in order to compare their sensitivity. Results Capillary electrophoresis detected the 1/600 of the PCR product from 20 picogram of male DNA, as amount of DNA in 3-4 cells.
结果 毛细管电泳可检测到20 pg男性模板DNA(相当于3-4个细胞中的DNA含量)PCR扩增产物总量的1/600,琼脂糖凝胶电泳可检测到74 pg男性模板DNA(相当于13个细胞中的DNA含量)PCR扩增产物总量的1/3。
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In addition nC〓, nC〓, two isomers of C〓 sterene and three isomers of C〓 isoprenoid hydrocarbons were also identified from aliphatic fraction of pyrolyzed product at 200℃. After pyrolyzed at 300℃, the main aliphatic hydrocarbons were normal alkanes ranged from C〓 to C〓, with C〓 as the main peak and C〓 as the second main peak. Meanwhile, pristane, phytane and C〓 sterane were also detected. In the pyrolysates at 400℃ and 500℃, the content of saturated hydrocarbons decreased, the range of normal alkanes distribution became narrow and the main peak was nC〓. Low concentration of C〓-C〓 steranes was detected and the content of C〓-C〓 increased more.
在200℃热模拟产物饱和烃馏分中除检测出两种长链烯烃外,还检测到正十九碳单烯烃、正三十五碳双烯烃、两个碳二十八甾烯的同分异构体和三个C〓类异戊二烯烷烃化合物同分异构体。300℃模拟产物饱和烃主要为正烷烃,正烷烃的碳数分布范围为C〓-C〓,以C〓为主峰、C〓为次主峰;另外,样品中还检出姥鲛烷、植烷和C〓甾烷等化合物。400和500℃热模拟产物中的饱和烃含量下降,正烷烃系列碳数分布范围变窄,主峰碳后移至C〓,检测到低浓度的C〓-C〓甾烷系列化合物,另外,C〓-C〓藿烷系列化合物含量进一步增加。
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A to-be-detected plate (25) for prevention of rear-end collision is provided at the rear end of the transport mover V. The to-be-detected plate (25) can enter a space between the proximity sensors (23, 24) and the decelerator/stopper W.
在各运输用电车V的后端,设有进入第1及第2非接触式传感器23、24与减速/停止装置W间的防追尾用被检测板25。
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Spermine and spermidine were detected in fairly high amounts in the muscle. In the liver and gonad putrescine was detected in the highest amounts, followed by spermidine and spermine.
肌肉中的多胺检出为精胺和亚精胺,肝脏和生殖腺中有较高浓度的腐胺及亚精胺和精胺检出。
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Quality supervision department asked enterprises of all stocks of dairy raw materials inventory and registration of melamine can not provide proof of dairy products were not detected in raw materials, must be approved by the test to determine melamine not detected before use.
质监部门要求企业对所有库存的乳制品原料进行清查,并登记备案,对不能提供三聚氰胺未检出证明的乳制品原料,必须经检验确定未检出三聚氰胺后方可投入使用。
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1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.
1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异;采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异;采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。
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- 推荐网络例句
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The changes of the HAF after liver injury may be related to the volume of blood loss, the intraabdominal pressure and the pneumoperitoneum duration.
肝撞击伤伴失血后肝动脉血流量的变化与失血量、气腹压力以及气腹持续时间等有密切联系。
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Reneging on our commitments to the world's poor cannot be an austerity measure, she said.
放弃我们给世界上的穷国的承诺不能成为一种紧缩措施。
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Abama respond ed by saying that he ***n and Patrick a are friends and that they often share ideas that re talk and rhetorical flourishes ***Also Although most independ ent ponds pundits view ed the charge pregs *** of plagiarism as exaggerated they did see possible damage to abama's image in the the eyes words of the voters, because he sawing *** his soaring oratory has been his stronger strongest ased asset .
奥把马回应到:他们是哥们,经常分享一些主意和修辞方法。虽然一些独立专家认为剽窃言过其词了,他们仍然认为这可能损害阿宝马在选民中得形象,因为修辞好是他得有力武器。