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Methods: four male volunteers were recruited to live in a disabled submarine simulator which is close, dark and algid for 10 days. they were supplied 1.5 l water and one portion of saving life dry provision everyday. the body weight was detected before the experiment and on day 3, day 4, day 5 and day 10 after exposure. at the same time, venous blood taken suction from elbow was used to detect the level of blood glucose, which was centrifuged to take the upper serum to detect the level of ffa at low temperature by colorimetric.

4名男性受试人员在封闭、黑暗、寒冷的试验舱体中进行为期10 d的模拟失事潜艇环境实验,每人每天供水1.5 l和1盒救生压缩干粮,实验前和第3、4、5、10天出舱的晨起测定体质量,抽取肘部静脉血,测定血糖;低温离心留取上层血清,采用一次提取比色法测定游离脂肪酸。

Methods ICH was induced in rats using stereotactic infusion antilogous blood 50μl into the caudate nucleus. The male rats were randomly divided into control group and hemorrhage group. TUNEL method was used to detect apoptosis, and immunohistochemistry method to detect the expression of Caspase-3、 Ref-1 in cerebral tissues at different time.

采取立体定向技术,将SD大鼠自体不凝血50μl注入大脑尾状核区制备脑出血模型,将大鼠随机分为正常组和出血组,分别在不同时间点断头取脑,连续切片作TUNEL、Ref-1和Caspase-3免疫组化染色。

Secondly, 4 methods of azimuth estimation based on vector hydrophone according to forms of nosie and signal are brought forward. The principles of these 4methods are set forth, simulating them by computer. Acoustic intensity averager can effectively suppress isotropic non-coherent interference; DOA based on line-spectra can effectively detect the target radiating line-spectra and estimate its azimuth angle; Bar graph approach can suppress line-spectra coherent interference and distinguish different targets radiating line-spectrum. Weighted bargraph approach can detect target in broadband coherent interference and estimate its azimuth angle.

其次,根据背景干扰和信号样式的不同,提出了单矢量水听器方位估计的4种方法;阐述了这4种方法的原理,并进行了计算机仿真,指出平均声强器方位估计适用于各向同性噪声场背景干扰,讨论了平均声强器的处理增益;目标辐射噪声中有线谱时,线谱方位估计有很好的性能,其中的LOFAR线谱和高频解调得到的DEMON线谱都是目标的特征谱线,可以利用它们进行方位估计,详细讨论了利用特征线谱单个矢量水听器分辨多目标的性能;直方图方位估计能抗强线谱相干干扰;加权直方图方位估计可以抗宽带相干干扰。

MethodsThe cells of human gastric cancer cell line SGC7901 was treated respectively with Chrysin at different concentration 10、20、40、80μM for 48h. The proliferation inhibitory rate was measured by MTT assay. Acridine orangestaining and flow cytometry were used to detect apoptosis. Western blot assay was used to detect the expression of apoptosis related genes NF拨B,Bcl2,Bax.

方法分别用10、20、40、80μM的ChR处理人胃癌细胞株SGC7901 48h,采用MTT比色法检测ChR对SGC7901细胞的增殖抑制效应;采用丫啶橙染色、流式细胞术检测ChR诱导SGC7901细胞凋亡的发生;应用Westernblot法检测凋亡相关基因NF拨B、Bcl2、Bax的蛋白表达,并用计算机图像分析软件进行半定量分析。

1H-MRS can detect brain metabolic changes in patients with cyanosed congenital heart disease in vivo noninvasively and can detect the metabolism disorder of the energy and amino acid,so the pathophysiology of this disease can be understood.

磁共振质子波谱能无创性地观察到紫绀型先天性心脏病患儿脑代谢异常的部分产物,可揭示慢性缺氧对脑组织代谢的影响及脑损害的病理生理机制。

Luciferase reporter gene method was used to detect AP-1 transactivation activity in p53 normal or defunction L02 cells at different time points and DEN concentrations, to detect the influence of p53 transactivation activity by DEN pretreatment at different time points, and the influence of AP-1 transactivation activity by PFT-α.

采用荧光素酶报告基因法检测不同时间点、不同浓度DEN对p53功能正常和缺失的L02细胞AP-1转录激活活性的影响,检测不同DEN处理时间对p53转录激活活性的影响及PFT-α对AP-1转录激活活性的影响。

Methods Realtime fluorescence quantitive RTPCR was used to detect the expression level of Tiam1 mRNA in gastric cancer tissues of 66 cases, paracancerous tissues (over 3cm) of 66 cases and gastric benign lesion tissues of 11 cases. Meanwhile, CK18 was selected as the hallmarker for lymph node micrometastasis to detect the level of CK18 mRNA expression in 199 lymph nodes from 24 cases of gastric carcinomas diagnosed negative by pathologic examinations.

采用TaqMan探针实时荧光定量PCR方法,定量检测66例胃癌患者癌组织、癌旁组织及11例胃良性病变组织中的Tiam1 mRNA拷贝数,同时将CK18作为淋巴结微转移的标志基因,定量检测24例病理诊断无淋巴结转移的胃癌患者199枚淋巴结的CK18mRNA拷贝数。

Routine CT and MR examinations can only detect infracortical cerebral arteriosclerosis, whereas CT perfusion mapping images can detect evident slowed down cerebral focal blood flow Pictures 3, 4, 7, 8

常规CT和MR检查仅可见皮层下动脉硬化性脑病,CT灌注图像可见明显的脑局部血流缓慢(图3,4,7,8)。

Routine CT and MR examinations can only detect infracortical cerebral arteriosclerosis, whereas CT perfusion mapping images can detect evident slowed down cerebral focal blood flow

常规CT和MR检查仅可见皮层下动脉硬化性脑病,CT灌注图像可见明显的脑局部血流缓慢。

Routine CT and MR examinations can only detect infracortical cerebral arteriosclerosis, whereas CT perfusion mapping image s can detect evident slowed down cerebral focal blood flow

常规CT和MR检查仅可见皮层下动脉硬化性脑病,CT灌注图像可见明显的脑局部血流缓慢。

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