查询词典 destructive effect

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

First of all, this paper introduces the whole design project and interrelated knowledge about Non-destructive detection, ultrasonic detection, and Phased-Array ultrasonic detection Then the paper depicts the principle of Phased-Array focus and abstracts a set of math model and feasible arithmetic on the base of the real parameter of the system. Put forward several problems that exist during the course of system development and program. Introduce some measures of distinguish that are often used in non-destructive detecting and echo wave crest. Then I depict one of the measures in detail that used in the course of non-destructive detection, which is the way of distinguishing whether defects exist or not. Then provide part core program to realize it. This paper also provides main flow chart through analyzing how main control program controls different parts to make the whole system more effective.

然后在文中以较大的篇幅，描述了相控阵聚焦的原理，并根据该系统的实际参数，抽象出一套数学模型和可行的算法，在此基础上提出了在系统开发过程及编程实现过程中存在的一些问题；介绍了在无损检测中缺陷的分类和对回波峰常用的识别方法，详述了系统开发过程中使用的其中一种识别方法，即判别是否存在缺陷的算法，并给出实现之的部分核心程序；详述主控程序如何控制各部分并协调各相关软件的工作，使整个系统发挥最高效能，并给出主要设计框图和程序。

The competent administrative departments or institutions for seismic work under the local people's governments at or above the county level in areas where destructive earthquakes may occur shall, together with the departments concerned, work out emergency preplans for destructive earthquakes in their own administrative areas in the light of the national emergency preplans for destructive earthquakes and submit them to the said people's governments for approval.

可能发生破坏性地震地区的县级以上地方人民政府负责管理地震工作的部门或者机构，应当会同有关部门参照国家破坏性地震应急预案，制定本行政区域内的破坏性地震应急预案，报本级人民政府批准

The effect of treatment is negatively correlated with the course of disease, generally, the effect is much worse with the course of disease is much longer; The effect is positively correlated with the course of treatment, it is better if the course of treatment is longer.The group of more than one year is much excellent than the group of less one year. The ex-electroencephalogram before treatment is normal, on edge and a little abnormal, the effect is better. As regards CT or MR result, there is difference between the effective group and the ineffective group. The result of CT and MR is negative, the effect is better, and the effect to demyelination and leukoencephalopathy is most worst.However,for the tipe of much abnormality, the effect is the most worst.The effect is related to course of disease.The effect is better if the course is shorter.There is no difference in the tipe of disease the symptoms before episode, the frequency of episode, the inducing reasons, the age, sex and so on.

病程的长短与疗效存在负相关关系，病程越长疗效越差；疗程与疗效存在正相关关系，疗程越长疗效越好；疗前脑电图的异常程度与疗效存在负相关关系，正常、边缘及轻度异常者，疗效较好，重度异常者疗效最差；无效组与有效组在CT及MR检查结果上有差异，有阴性者疗效好，阳性者疗效差，脱髓鞘及脑白质病疗效最差的趋势；疗效与性别无相关性；无效组与有效组在发作类型、既往病史、辨证分型、发作先兆、疗前发作频率、诱因、发病年龄、就诊前所用药物方面无差异。

As a whole, this article investigates the investment effect problem from a new view of regional economics. It covers the economic, social and ecological effect of regional investment; it defines the concepts of investment effect and regional investment effect, deciphers the connotation, classification and influential factors of investment effect, and establishes a basic theory framework of investment effect research; constructs a fire-new regional investment effect evaluation model that could evaluate comprehensively the economic, social and ecological effect of regional investment, including evaluation index system and evaluation method, which supplies the gap of regional investment effect evaluation in academic community.

总体上看，本文从区域经济学这一新的视角研究投资效应问题，内容涵盖了区域投资的经济效应、社会效应和生态环境效应；界定了投资效应、区域投资效应的概念，解读投资效应的内涵、分类与影响因素，建立了投资效应研究的基本理论框架；构建一个对区域投资的经济效应、社会效应和生态环境效应进行综合评价，包括评价指标体系和评价方法在内的全新的区域投资效应评价模型，弥补了学术界在区域投资效应评价方面的空白。

In the dissertation, the study on carbon fiber reinforced concrete andplain concrete has been reviewed. Then mechano-electric effect of CFRC and plainconcrete, its mechanisms, the interaction among mechano-electric effect,piezoresistivity and seebeck effect, the electrical circuit model of concrete based onmechano-electric effect, electromagnetic emission of concrete, the mathematicalmodel of mechano-electric effect are investigated. Also the converse effect ofmechano-electric effect of CFRC and plain concrete, that is, electromechanicaleffect, and the interaction between electromechanical effect and electrothermaleffect of CFRC are examined.

本文对碳纤维混凝土和素混凝土机敏性的国内外研究现状进行了全面的评述，在大量实验分析的基础上，系统地研究了碳纤维混凝土和素混凝土的力电机敏性，研究了力电效应机理、力电效应与其它效应(压阻效应、塞贝克效应等)的耦合关系、基于力电效应的混凝土电学模型、混凝土电磁发射现象、混凝土力电效应的数学模型等；论文同时对混凝土和素混凝土的电力效应、碳纤维混凝土电力效应与电热效应的耦合关系等进行了探讨；最后开展了基于力电效应的机敏混凝土结构应用研究。

This paper brings forward a non-destructive measurement method based on bilinear mapping which aimed at problems of how to correct image and wipe off texture feature to conduct non-destructive measurement of soybean-leaf area using computer vision technology.

该项研究针对大豆叶面积无损测量中校正图像和去除叶片纹理特征等问题上，提出了基于双线性映射的无损测量法。

If enough Americans can grasp the destructive charlatanism in play here then the truth can eventually be known and Cheney and Bush, along with their accomplices, will ultimately have to begin paying the ultimate price for destructive global and domestic policies grounded in deceit.

如果足够多的美国人能够把握破坏性charlatanism在这里打球的话，真相最终可以称为切尼和布什，并附上自己的同夥，最终将要开始支付的最终价格进行破坏性的全球和国内政策奠基於欺骗。

In product qualiy judgement of sample Lests, when the objective indicators get its values required destructive tasting, it is usually use the covariant index from non-destructive testing to forecast.

在产品质量判定的抽样检验问题中，当目标指标需用破坏性试验才能得其值时，更为常用的足用非破坏性试验可得量值的协变指标量来预报它。

All locks acquired by statements within a transaction are held for the duration of the transaction, preventing destructive interference including dirty reads, lost updates , and destructive DDL operations from concurrent transactions.

事务内各语句获得的锁在事务执行期内有效，以防止事务间破坏性的相互干扰，例如：脏读取，无效地更新，以及其他并发事务中具有破坏性的 DDL 操作。

Who? I never heard of him, Paul said, before asking teammate James Posey if he had heard of him.

赛后，科比说，他一直都是一名非常出色的射手，今天他打得很棒。

When I joined the company, I rotated around the different sections.

我加入这个公司时，轮换过几个不同的部门。