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The AC2 gene fragment of cotton leaf curl virus was obtained from total DNA of CLCuV infected tobacco leaves by polymerase chain reaction, and the amplified DNA fragment was cloned into vector.

以棉花曲叶病毒侵染的烟草叶片组织总DNA为模板，通过聚合酶链反应扩增CLCuV的AC2基因片段并插入克隆载体。

Tomato yellow leaf curl China virus ; DNA-A ; genetic diversity

中国番茄黄化曲叶病毒； DNA-A ；多样性

Isolation, Sequence Analysis, and Preliminary Functional Studies of a Novel Bidirectional Promoter, Cotton Leaf Curl Virus Promoter

棉花曲叶病毒(Cotton Leaf Curl Virus， CLCuV)是新近发现的一种单链DNA病毒即双生病毒，最近几年在印巴次大陆危害严重。

Complementary sense promoter from cotton leaf curl virusis a novel promo ter and could direct high level expression of foreign genes in transgenic plants .

摘 要 棉花曲叶病毒互补链基因启动子是一种新近鉴定的启动子，它能驱动外源基因在植物体内高效表达。

The thesis decribes two methods of detecting CLCuV-PK: TAS-ELISA and PCR. it is shown that CLCuV-PK is very closely related to viruses causing yellow vein disease or leaf curl disease of okra in Pakistan.

本文用二种方法检测CLCuV-PK，证明CLCuV-PK与引起巴基斯坦秋葵曲叶和秋葵黄化叶脉花叶的病毒有很近的亲缘关系。

Recently, the Cotton leaf curl Multan virus was found to seriously infect Hibiscus rosa sinensis and Abelmoschus esculentus in Guangdong and Guangxi.

近年来，在广东和广西分别发现了严重侵染朱槿和黄秋葵的木尔坦棉花曲叶病毒。

At first, plants would turn dark green but soon the top leaves would progress through typical K symptoms to bronzing and leaf curl.

最初植株变得深绿，但很快上部叶片就会经历典型缺钾症状出现青铜症和卷叶。

The monomer and dimer clones of full-length genome of cotton leaf curl gemini-virus from Pakistan were constructed , and it was shown that CLCuV-PK could be a geminivirus with monopartite genome.

本研究完成了巴基斯坦棉花曲叶病毒全长基因组单体和双体DNA克隆，证明了CLCuV-PK是一个单组分双生病毒。

The results indicated that the incidence of virus disease in processed tomato was 63%~91% from 2005 to 2006. The field symptoms had various disease symptoms including mosaic, leaf streak necrosis, fernbrake leaf and malformation, curl leaf and dwarf etc., but mainly mosaic and leaf streak necrosis with infectious frequencies of 78% and 37%, and leaf streak necrosis symptom with mosaic was above 90%.

结果表明：加工番茄病毒病害2005~2006年间各地发病率达63%~91%，在田间主要表现为花叶、条斑坏死、蕨叶畸形、曲叶和矮化等多种病害症状，以花叶和坏死条斑为主，其中花叶占78%，条斑占37%，并且条斑症状90%以上都伴有花叶症状。

The most serious damage caused by B. tabaci is its transmission of the Tomato leaf curl Taiwan virus to tomato plants in Taiwan.

台湾番茄卷病毒(Tomato leaf curl Taiwan virus， ToLCTWV)由烟草粉虱所传播，并在台湾地区造成番茄作物的严重损失。

Yet, Edith says, there was always money in the bank.

但是Edith说，她的银行户口里面总是有钱。

We have created a series of files that resolve this problem--one for each day of the 40 days of purpose.

我们制作一系列的档案去解决这问题---一个为标竿40天中的每一天。