查询词典 culture flask

The influence of dissolve oxygen to the yield of coenzyme Q_(10) in flask culture was studied.

考察了发酵过程中溶氧对辅酶Q_(10)产量的影响。

The steps for preparing insecticide include: first shaking flask for fermentation, adding amidon, soya bean cake powder, cotton cake powder, protease leather, yeast powder, maize milk, magnesium sulfate heptahydrate, monobasic potassium phosphate, dibasic potassium phosphate, calcium carbonate alpha-amylase and make-up water by ratio in culture medium with 100 unit volumes; secondarily filling 100ml culture mediums in triangular bottle, then seeding BTH-13 after disinfection and culturing them until the brood-cell pulls off under finite temperature; third adopting the selected culture medium and fermenting them in full-automatic fermentation tank, acidifying the fermentation liquor after the fermentation, adding the emulsifier, and directly performing spray-drying after the compression of the fermentation liquor to prepare the wettable powder.

制备杀虫剂的步骤是：首先是摇瓶发酵，在100单位体积的培养基中，按比例加入淀粉、豆饼粉、棉饼子粉、蛋白胨、酵母粉、玉米浆、七水硫酸镁、磷酸二氢钾、磷酸氢二钾、碳酸钙、及α-淀粉酶，补充水；其次是在三角瓶中装100ml培养基，灭菌后接种BTH-13，在一定温度培养至芽孢脱落；第三是采用筛选出来的培养基，在全自动发酵罐中进行发酵，发酵结束后发酵液酸化，加入乳化剂，酵液浓缩后直接喷雾干燥制备可湿性粉剂。

B order to make full use of Ta1, it is essential to improve the investigation to industrial production scale by drawing assistance from high cell density fermentation. Pilot test of tube culture and flask shaking culture were done to get optimized culture conditions. Finally, we reached the goal of high cell density fermentation and high level expression of recombinant human Ta1 E.

在经过试管以及三角摇瓶水平对培养条件进行摸索后，我们成功地实现了该工程菌的高密度发酵，3乙菌液获得湿菌体工4口丁蛋白表达水平与试管水平相当。

Results the optimized culture conditions as follows: 10 g/l glucose, 10 g/l yeast extract powder, alcohol=10 %, ph 5.5 , culture 5 d quiescently with 50 ml medium in 500 ml flask.

结果优化培养条件为：葡萄糖10 g/l，酵母膏10 g/l，乙醇10%，ph 5.5，装量50 ml/500 ml，静置培养5 d。

The control effects were above 66%. According morphologic, physiological and biochemical characteries, strain YN-55, NN-3, YN-4 and YN-10 were identified.Some conditions for culture YN-55, NN-3, YN-4 and YN-10 were studied in shake flask (100ml in 150ml flask).

本文根据形态特征和生理生化性状，初步鉴定内生细菌YN-55为假单胞杆菌属铜绿假单胞菌，NN-3为芽孢杆菌属枯草芽孢杆菌，YN-4、YN-10为芽孢杆菌属凝结芽孢杆菌。

The three parameters in the culture of 1 000 mL flask were 27%,30%,and 27% lower than that of 250 mL flask,respectively.The concentrates of phosphate,nitrate and ammonium in progressive scale-up flasks decreased similarly.

在逐级放大的摇瓶中，氮、磷、铵浓度都随着培养时间延长而逐渐降低，尽管在1L的摇瓶中磷消耗得最慢，但三种摇瓶中磷在细胞生长对数期基本都被消耗尽了。

The third passage osteoblasts were plated at the density of 5×104/ml in 75ml culture flask, twenty four hours later treatment factors were added. Total RAN was extracted from osteoblasts using guanidine, and mRNA expression of OPG and RANKL were examined using RT-PCR.

OPG作为RANKL的诱骗受体阻断其与表达于破骨细胞前体细胞和成熟的破骨细胞表面的功能性受体RANK的作用，从而抑制破骨细胞的分化和功能。

The third passage osteoblasts were plated at the density of 5×104/ml in 75ml culture flask, twenty four hours later treatment factors were added.

许多激素及细胞因子通过调节破骨细胞和成骨细胞这两类细胞的数量和活性，使骨量维持动态平衡。

Through shake flask experiments,it was acquired that the best culture conditions:pH6.5,37℃,240rpm,40mL/300mL,and 2.5%of bacterium culture for 12h.

通过摇瓶发酵试验，确定其最佳培养条件为：摇床37℃，240rpm，发酵培养基的起始pH为6.5，接种量为2.5%，装液量为40mL/300mL，培养32h。

With the single factor test and orthogonal test, the optimal culture media for HF3-04 strain producing agarase was decided as: agar 3‰, NH4NO31‰, Na2HPO4 0.5 mM, pH 8.0. The optimal fermentation conditions were inoculum size 3‰, 110ml medium in 250mL Erlenmeyer flask, rotation rate 150r∕min, culture time 48h,culture temperature 25℃.

通过正交试验确定其最佳培养基组成为琼胶3‰、硝酸铵1‰、磷酸氢二钠0.5mM、pH值8.0；该菌株的最佳培养条件为接种量1%、装液量为250ml三角瓶装入110ml发酵培养基、摇床转速150r∕min、培养温度25℃、培养时间48h。

I think he's learned how to approach hitters....

Johhny Damon说，『他已是最好的了，再没有人能够比他做的更好，我想他的球技已经完全成熟了，而且他也已经学会了如何去对付和压迫各种打击者。。。。。。

In effect, an 18 foot King Cobra could actually look down onto the average human being.

际上，一个18尺眼镜蛇王其实可以俯视到的平均人。