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To compensate the descent of pH for the heat-up, exalting the Tris-HCl buffer's pH can avoid the abscission of the purine and make the long sequence PCR go on smoothly. The GC-rich template's secondary structure is complex and stable. The organic reagent methylamine contributes to uncoil the secondary structure.The pfu DNA polymerase has more fidelity and thermostability than Taq DNA polymerase.

高GC含量使模板二级结构复杂而稳定，一般热启动难于解开，而变性温度过高、时间过长又不利于DNA聚合酶的活性作用发挥，于是借助能缓释模板二级结构的有机试剂甲胺扩增高GC含量模板。pfu DNA聚合酶比Taq酶有更高的保真复制性能和热稳定性，更有利于高GC含量模板的扩增。

It is to say that after mixing for a period of time, continuous mixing contributes quite small to the unconfined compression strength.

也就是说在搅拌一段时间后，持续的搅拌对强度贡献很小。

Actually, the development of genetically-modified products is a double-edged sword: On the one hand, it contributes to the enhancement of the world crop yield, as well as the quality, and reduction of chemical fertilizer and the pesticide use, helps solution of the famine and undernourishment in developing countries. Besides, it also promotes the development of certain related industries like pharmaceutical and chemical industries; On the other hand, it also brings the unprecedented threat for human beings. We are unaware of the potential effects in human bodies, or any unknown injury to the next generation, caused by genetic modification. Nor can we forecast what kind of influence to the extant gene will emerge.

而事实上，转基因生物技术的确是一把双刃剑：一方面，转基因技术可以提高世界农作物产量、提高质量、减少化学肥料和杀虫剂的使用、解决发展中国家的粮食缺乏、饥饿和营养不良的问题，另外，基因技术的飞速发展在很大程度上也促进了医药、化工等相关行业的发展；另一方面，它也为整个人类带来了前所未有的威胁，人们无法得知基因序列的改变会在体内引起何种潜在的变化，无法确定转基因产品是否会对下一代产生任何未知的伤害，无法预测转基因产品对现存基因会产生何种影响。

Actually, the development of genetically-modified products is adouble-edged sword: On the one hand, it contributes to theenhancement of the world crop yield, as well as the quality, andreduction of chemical fertilizer and the pesticide use, helpssolution of the famine and undernourishment in developingcountries. Besides, it also promotes the development of certainrelated industries like pharmaceutical and chemical industries; Onthe other hand, it also brings the unprecedented threat for humanbeings. We are unaware of the potential effects in human bodies, orany unknown injury to the next generation, caused by geneticmodification. Nor can we forecast what kind of influence to theextant gene will emerge.

而事实上，转基因生物技术的确是一把双刃剑：一方面，转基因技术可以提高世界农作物产量、提高质量、减少化学肥料和杀虫剂的使用、解决发展中国家的粮食缺乏、饥饿和营养不良的问题，另外，基因技术的飞速发展在很大程度上也促进了医药、化工等相关行业的发展；另一方面，它也为整个人类带来了前所未有的威胁，人们无法得知基因序列的改变会在体内引起何种潜在的变化，无法确定转基因产品是否会对下一代产生任何未知的伤害，无法预测转基因产品对现存基因会产生何种影响。

The newly built precision workshop has the capacity to produce high-speed precision low-noise bearings which are used in precision machines such as the main shaft of machine tools, large/medium motors and precision rolling mills, so as to substitute imported ones or make up domestic underproduction, which saves the cost for customers, shorten the receipt period and contributes to the industry development and technical advance.

新建成的精密车间可生产高速精密、低噪音轴承用于机床主轴、中大型电机、精密轧机等精密机械上，以替代进口或补充国内生产不足，为用户节约成本，减少进货周期，为产业发展和技术进步做出了贡献。

This finding coupled with the short-term tolerability of warfarin likely contributes to its underutilization.

这项发现加上华发林的短期耐受性试验可能对于解释它的少运用率有帮助。

It contributes to her oral speaking as well as being unembarrassed in front of audience.

这有利于提高她的口头表达能力以及练就在众人面前的胆量。

To approach security theory from feminist perspective, is unexceptionally peripheral compared with such mainstream theory of IR as realist security theory, but it contributes significantly to security studies both in theory and practice as well as the discipline of IR as a whole.

然而事实是，女性主义无论是对安全研究的理论与实践以及对国际关系这个学科都作出了巨大贡献。

UNHCR contributes to the protection of refugees to a great extent as a global international organization.

联合国难民署在阿富汗难民保护方面扮演了极为重要的角色。

The return to unity creates group relations in which all are supported and each contributes to the greater good of the whole.

回归到统一创造了所有成员都得到支持而每个成员都贡献给整体利益的群体关系。

He got into race cars, while my father liked go-karts more, so I didn't really pay attention to what Diego was doing, as I was just racing karts.

叔叔开的是跑车，而我父亲更喜欢越野卡丁车，所以我并不太关注Diego在做什么，我仅仅在跑我的卡丁车而已。

She is at present gone to look for the best nectar and ambrosia to regale me this evening.

她今晚为款待我，现在又去准备美酒佳肴去了。