查询词典 conditional expression

To test the hypothesis, neontal rat cadiomyocytes Treat with heat shock(HS,42℃,2h) to induce the expression of HSP70,then teated with 0.5mmol/L H2O2. We first found H2O2 can reduced cadiomyocytes apoptosis and HSP70 only over-expression in the HS group, other two group only express HSP70 a little in the test of expression of HSP70.Then we tested the concentration of Ca2+, the [Ca2+ ] of CON was 192.224+6.654, the [Ca2+ ] of H2O2 group was 290.6918+8.922and the [Ca2+]of HS group was 214.2633+4.484.To further determine how HSP70 repaired the Ca2+ homestasis, we measured calcium transient of each group.

实验中应用MTT和流式细胞技术检测细胞的凋亡，发现损伤组的存活率明显低于其他2组，热休克组(Heat shock group，HS group)细胞存活率略低于正常组细胞；免疫组化结果表明只有在HS组才检测到阳性反应，即是HSP70的表达，其他2组均为弱阳性结果；应用离子成像技术对细胞内钙离子浓度进行测定，发现损伤组290.6918+8.922明显高于正常组192.224+6.654和HS组214.2633+4.484，HS组细胞内的钙离子浓度略高于正常组；为了进一步探讨HSP70对于ROS引起的心肌凋亡过程中钙离子的调控机制，又对各组的心肌细胞进行了钙瞬变的测定。

This implies that DNA methylation of promoter exon 1_7 may not underly the mechanism of GR up-regulation in GLP-1 -programmed rats.5 Hippocampal expression of GLP-1R and NGFI-A mRNAHippocampal GLP-1R (p =.009) and NGFI-A (p =.001) gene mRNA expression were significantly up-regulated in GP group as compared to VP group.Taken together, neonatal intramuscular injection of plasmid DNA encoding GLP-1 affects behavior and hippocampal GR expression in adolescent rats.

提示GR exon 1_7启动子区域DNA甲基化可能不参与GLP-1导致的GR转录上调。6海马GLP-1R和NGFI-A mRNA表达GP组大鼠GLP-lR(P=。009)和NGFI-A(P=。001)表达均显著高于VP组，表明肌肉注射GLP-1可能通过作用于其海马受体提高NGFI-A表达，进而调控GR表达。

OC, BSP, OPN all showed low expression in unmineralized neoplastic bone matrix In mineralized neoplastic bone matrix, BSP and OPN showed high expression and OC showed low expression.

三者在未矿化肿瘤骨基质中均呈低表达，在矿化肿瘤骨基质中BSP、OPN呈高表达，OC呈低表达，在正常成熟骨中，三者均呈高表达。

Results ①p53 positive expression rate in cervical carcinomas 85.71%( 30 /35) was significantly higher than that in normal cervixes 6.67%( 2 /30),P= 0.001.②p53 expression is correlated with the differentiation state and pathologic grades.The positive expression rate of p53 in the cervical carcinomas of grades Ⅰ 57.14%(8 /14) was significantly lower than that in the cervical carcinomas of grades Ⅱ 86.67%(13 /15) and grades Ⅲ 100.0%(6 /6),P＜0.05.③The positive rate of PCNA in the cervical carcinoma of grade Ⅰ 64.29% was signifi cantly lower than that in the grade Ⅱ 93.33% and grade Ⅲ 100.0%,P＜0.05.Conclusion Our data indicate that the alteration of p53 is involved in cervical carcinoma.

结果 ①P53蛋白在宫颈癌组织中的表达率为85.71%(30 /35)，明显高于在正常宫颈组织中的表达率6.67%( 2 /30)，P = 0.001；②P53蛋白阳性表达率与宫颈癌组织的病理学分级有关，P53在宫颈癌Ⅰ级中的阳性表达率57.14%（8 /14）明显低于Ⅱ级86.67%（13/15 和Ⅲ级100.0%(6/6)，P＜0.05；③宫颈癌组织PCNA阳性表达率Ⅰ级64.29％明显低于Ⅱ级93.33％和Ⅲ级100.0%，P＜0.05。

Supplementation of folate in diets may reduce hyperhomocysteinemia induced by excess intake of methionine and decrease the expression of NF-κBP65 protein and monocyte chemoattractant protein-1 (MCP-1) in carotid common artery after balloon angioplasty, These data show that Hcy may exacerbate neointima hyperplasia after denuding injury by increasing the expression of inflammatory factors via the activation of NF-kB, Folate can reverse the effect of Hcy on the activation of NF-κB and the expression of MCP-1, an effect of preventing the restenosis postangioplasty.

而Hcy有可能通过激活核转录因子通路增加损伤血管组织炎症因子的表达而加重血管球能损伤后新内膜的过度增生过程，叶酸能对抗Hcy的上述作用，对高同型半胱氨酸血症引起的血管成形术后再狭窄可能有一定的预防作用。

One is that non-expression does not mean inward suppression. It means disallowing outward expression. The other point is that non-expression is but a first step.

这项解释形成了工作负面情绪的基础，但还有另外两点值得一提：其一，不表达并不是内心的压抑，它只是不准许外在的表达。

Methods Immunohistochemistry was employed to detect the expression of RASSF1A gene in 39 cases of human gastric cancer tissues and 18 cases of normal human gastric tissues, and RT-PCR was used to examine the expression of RASSF1A mRNA in 4 gastric cancer cell lines, normal gastric cell lines GES-1 and positive control Hefts cells. Correlations between expression of RASSF1A gene and clinicopathological characteristics of gastric cancer were also analyzed.

应用免疫组化法分别检测39例胃癌组织标本和18例正常对照胃组织标本RASSF1A蛋白表达；RT-PCR法检测4株胃癌细胞株、正常胃黏膜细胞株CES-1以及阳性对照Hela细胞中RASSF1A mRNA的表达；结合病理学特征进行分析。

OLH or 8-bromo-cAMP were added to luteinized granulosa cells. Results indicated that, protein expression of HIF-1a, HIF-1b and VEGF had a better performance in hypoxia than in normoxic condition, but phospho-PKA, StAR and P450scc protein expression had weaker expression in the former condition than in the later one. As a result of decreasing secretion of progesterone the decreasing of phospho-PKA was suspected to have a role in the reduction of StAR and P450scc in the condition of hypoxia.

结果发现，黄体化粒性细胞於缺氧条件下其HIF-1a、HIF-1b与VEGF蛋白质表现量皆较正常氧高；同时，StAR及P450scc蛋白质表现量则降低，但3b-HSD不受缺氧影响；此外，藉由特异性抗体检测PKA讯息传递路径发现，PKA讯息传递路经於缺氧条件下磷酸化PKA（phospho-protein kinase A）蛋白质表现量下降，可能经此路径的调控造成StAR及P450scc蛋白质表现量下降，导致黄体化粒性细胞孕酮分泌量减少。

When expression the knowledge we integrate object oriented expression and produce formula expression, and flexible deal with knowledge with different profundities.

在知识的表示中，集面向对象的表示和产生式表示为一体，并对不同深度的知识予以灵活处理。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清，进行以下检测：(1)原子吸收法测血清锌浓度；(2)测胸腺、脾脏脏器重量并与大鼠体重比较，计算脏器指数；(3)HE染色对胸腺、脾脏进行病理观察；(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡，并通过图像分析进行比较；(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达；(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达；(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。