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The goal of this dissertation is to develop a novel freshwater clam Corbicula fluminea-based on-line bimonitoring system as a bioassay tool to offer a real-time and cost-effective method to measure copper and cadminm concentrations in natural water resources.

本论文提出一崭新淡水亚洲蚬为基础之线上生物监测系统作为生物鉴定工具，提供一即时且有效低成本量测法以侦测自然水资源中铜或镉浓度。

MPS is stored in connective tissue cells, fibers, and the ground sub-stance of corium and subcutis, where, over longer periods of time, it reaches concentrations which must be considered biologically active.

MPS储存于结缔组织细胞、纤维组织以及真皮和皮下组织基质，并在经过更长一段时间后在该处达到具有生物活性的浓度。

Hemoglobin concentrations and mean corpuscular volume were tracked from infancy to childhood.

从婴儿期到儿童期血红蛋白浓度和平均红细胞容积有履带性。

The cytoplasmic trehalose concentration in red blood cells, free hemoglobin and ATP level were determined at different incubation temperatures (4, 22 and 37℃), different trehaolse concentrations (0, 200, 400, 600, 800 and 1000 mmol/L ) and different incubation times (2, 4, 6, 8 and 10 hours), the cytoplasmic trehalose, free hemoglobin, hemoglobin and mean corpuscular volume in fresh RBCs and RBCs strored for 72 hours at 4℃ were compared, when loading condition was ensured.

在不同孵育温度（4、22和37℃）、孵育时间（0、2、4、6、8、10小时）、不同负载缓冲液浓度（0、200、400、600、800、1 000 mmol/L）条件下检测新鲜红细胞对海藻糖的成功负载量及红细胞各项理化指标；在固定负载条件下，对新鲜红细胞和4℃保存72小时红细胞海藻糖负载、游离血红蛋白、血红蛋白和红细胞平均体积进行了比较。

Stationary source emissions - Automated monitoring of mass concentrations of particles - Performance characteristics, test methods and specifications; Technical Corrigendum 1

固定源辐射。自动监测粒子的质量浓度。性能特性、实验方法和规范、技术勘误1

The results show that the benthons were not polluted by the heavy metals, but the concentrations of Pb and Cd in crustacea and mollusk were potentially serious. The contents of Cd in crustacea was beyond the Standard Indexes of the seafood. The contents of Cd in mollusk was same as Stan...

结果表明，底栖生物中Hg、Pb、Cd、As含量较低，但甲壳动物和软体动物体内Pb和Cd具有潜在的严重积累问题；甲壳类Cd含量已严重超人体消费标准，软体类的Cd含量等于人体消费标准值，甲壳动物和软体动物的可食性应引起人们的关注。

In this paper,the treatment of water stress to Cryptomeria japonicaD.Don seeds was made by the different concentrations of polyethylene glycol.

本文采用配置不同浓度的 PEG溶液来模拟土壤自然水势，对日本柳杉种子萌芽实验进行人工水分胁迫处理。

Methods: The effects of initial and mature biofilms were measured by crystal violet assay under various concentrations of ampicillin and moxifloxacin.

通过结晶紫染色测定吸光度值的变化，观察不同浓度下两种抗生素对早期及成熟生物被膜的影响。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

The observational and calculated results are well matched. The calculated mean diameter, mean square-root diameter, mean cube -root diameter and liquid water content are all well matched with the observational results; In the paper two examples have been analyzed by using the seeding region data and the possible affecting region data in leeward region. It is found that F-100, 2dc, and 2dp's particle concentrations increase in leeward region, but rising extent is different, which is likely to be due to difference of seeding effect time.

拟合值和观测值吻合的较好，计算出的平均直径、均方根直径、均立方根直径和粒子浓度与观测值也比较吻合；本文对两组个例的催化影响区域及可能采集到相应值的下风方区域进行了分析，催化下风向影响区的F-100、2DC、2DP的粒子浓度比背景值都有所增加，但是增加的幅度不同，应该是催化响应时间的原因。

I go to school at night.

我是上上学。

Clear Tip Nasal Aspirator: A soft time and gentle suction action makes removing excess mucus easy.

鼻吸明确提示：一个柔软的时间和温柔吸力行动使消除过剩的粘液容易。