查询词典 comma bacillus

It was found that 0.25%o potassium sorbate produced a positive inhibition against short G+ spore bacillus with a concentration less than 5xl04cfu/ml. A concentration less than %o potassium sorbate hardly exerted a complete control towards short G- plump bacillus having a population density of 5x104cfu/ml. It was proved that use of l% potassium sorbute never controlled the growth of G+ coccus, G~ spirilla and enterobacter with a population density of 104cfu/ml. 1mmol EDTA completely controlled the growth of G+ short spore bacillus and G+ coccus whose cell density was 5xl04cfu/ml. A level of lmmol EDTA showed a limited inhibition against the growth of G- spirilla with a population density of 105 cfu/ml. However, a level of 10mmol EDTA completely controlled the growth of the G- spiral bacteria having a population density of 105cfu/ml. lOmmol EDTA produced a very significant control towards the growth of G"" plump short bacillus with 105cfu/ml. 20mmol EDTA showed a remarkable inhibition against the enterobacter with a population density of 105cfu/ml. Different concentrations of nisin including 25mg/mL, 50mg/mL, 75mg/mL and 100mg/mL were used as bio-preservative to examine its effects against the growth of all strains leading to the spoilage of fresh mutton meat. It was seen that there was a big difference in nisin's concentrations in inhibiting the spoiling bacteria. Generally speaking, as more as 75mg/mL of nisin significantly inhibited the growth of G+ short spore bacillus, G-plump short bacillus, enterobacter, G'spiral bacteria and G+ coccus having a population density of 105cfu/ml.

分别运用山梨酸钾、EDTA和Nisin对7种主要引起羊肉腐败的微生物进行了抑菌实验，结果显示，0.25‰以上山梨酸钾能够有效抑制5×10~4 cfu／mL以下的革兰氏阳性短芽孢杆菌的生长；1‰以下的山梨酸钾不能完全抑制5×10~4 cfu／mL革兰氏阴性粗短杆菌的生长，对10~4 cfu／mL革兰氏阳性球菌菌株、革兰氏阴性螺旋菌菌株和肠杆菌菌株抑制效果不太明显。1mmoL EDTA能完全抑制住小于10~5 cfu／mL革兰氏阳性短芽孢杆菌菌株、革兰氏阳性球菌菌株的生长，能明显的抑制10~5 cfu／mL的革兰氏阴性粗短杆菌菌株生长，对10~5 cfu／mL的革兰氏阴性螺旋菌菌株有一定的抑制作用。10mmoL EDTA能完全抑制住10~5 cfu／mL革兰氏阴性螺旋菌菌株的生长；能明显抑制10~5 cfu／mL的革兰氏阴性粗短杆菌菌株的生长，而20 mmoL EDTA能很明显抑制10~5 cfu／mL肠杆菌菌株的生长。25mg／mL、50 mg／mL、75 mg／mL和100 mg／mL的Nisin几乎对所有引起羊肉的腐败菌有抑制作用，但抑制程度不同，抑菌活性有一定的变化。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

Grounded on the first chapter, new and original in content, the second and the third chapters, employing the theories in phonetics, writing, rhetoric, semantics and aesthetics, further discusses the rhetorical and the semantic functions of the comma in English and Chinese: rhetorically speaking, the comma can be used to clarify, to expound, to substitute, to emphasize, to contrast, to complement, or to vivify; semantically speaking, the comma itself can carry information to indicate certain meaning, or the presence and absence of the comma can cause semantic changes. The fourth chapter applies what has been discussed in the previous chapters to English-Chinese translation.

在此基础上，本文第二、三章标新立异，运用语音语调原理、写作、修辞、美学、语义学等方面的知识，通过详实的文献实例，全面地论述了英汉语逗号的修辞和语义功能：从修辞功能的角度看，逗号可以用以进行澄清、解释、替代、强调、对比、补充，或是取得生动和幽默的效果，等等；从语义功能的角度看，逗号本身可以作为信息载体，暗含特定的意思，在更多的情况下，它本身不含意义，但它的使用与否会引起整个句子意思的变化。

Meanwhile, a new biger genetic group containing 6 cilicate sWains was formed, and it was different from the reference strains. The phylogenic tree of the representative sWains HLJ01 (GenBank accession EF031269) and HB 16 (GenBank accession EF031270) was constructed by analysis of the 16S rDNA full sequence, the results of analysis of 16S rDNA sequence revealed that swain HLJ01, HB 16 and Bacillus mucilaginosus and Bacillus edaphicus were located in the same phylogenic branch. HLJ01 and HB16 had high homology with Bacillus mucilaginosus in the phylogenic tree, but the 16S rDNA sequence of HLJ01 only has the identity of 96.7% with Bacillus mucilaginosus, and of HB16 has the identity of 95.5% with Bacillus mucilaginosus.

根据16S rDNA-RFLP聚类结果，选取供试硅酸盐细菌菌株HLJ01和HB16作为代表测定了其16S rDNA全序列，其Genbank序列登录号分别为EF031269和EF031270.16SrDNA序列分析表明：代表菌株HLJ01与HB16虽然与胶质芽胞杆菌位于同一个系统发育分支，但相似性分别只有96.7％、95.5％；与土壤芽孢杆菌的相似性则分别为94.6％和93.3％。

According to the identification, Bacillus subtilis CGMCC No.1982 is a typical Bacillus subtilis. Compared with other Bacillus subtilises, Bacillus subtilis CGMCC No.1982 has broad-spectrum antipathogen fungal activity; the fermentation broth and spore have sound effect in preservation of fresh fruits such as peach, grapes, etc, and also have sound antiviral effect in production of cucumber, tomato and other greenhouse vegetables.

经鉴定枯草芽孢杆菌CGMCC No.1982属于典型的枯草芽孢杆菌，与其它枯草芽孢杆菌相比其具有广谱抗病原真菌活性，其发酵液和芽孢对鲜桃、葡萄等水果保鲜效果较好；用于黄瓜、番茄等大棚蔬菜的生产抗病效果较好。

For example, it is a pathogenic bacterium and has endotoxins; it secretes protein into the periplasm and often into inclusion bodies. Bacillus subtilis is an attractive host for the production of heterologous secretory proteins. Many genes has succeed expressed in Bacillus subtilis, and the plasmid vectors for Bacillus subtilis are now taken from other genus bacterium, such as Staphylococcus aureus or lactococcus lactis and so on. As it is clear to known that these plasmids are far from optimal for Bacillus subtilis, because of low efficiencies of cloning and high levels of replicating instability.

枯草芽胞杆菌是目前原核表达系统中分泌表达外源蛋白较理想的宿主，近几年已在枯草芽胞杆菌中成功表达了多种基因，但大多都应用来源于金黄色葡萄球菌、乳酸杆菌等的载体来表达外源基因，由于其在枯草芽胞杆菌中存在分离复制的不稳定及外源基因的克隆表达效率低的现象，故都不是枯草芽胞杆菌的最佳选择，还有待改进。

The results of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L,there existed some stable hydrophobic regions,which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid,in the partially folded intermediate of Bacillus amyloliquefaciensα-amylase;with the denaturation concentration increasing,the stable hydrophobic regions disappered.the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed that using acrylamide as quenchers,with the protein denaturation extent increasing,the number of Trp that can be quenched increased untill all the Trp residues were quenched;Using potassium iodide as quenchers,with the maximum number(8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciensα-amylase molecule could be quenched by potassium iodide;with the denaturation concentration increasing,the number of Trp that can be quenched decreased to 5.the results of their protein electrophoreses and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciensα-amylase formed during the whole unfolding/refolding procedure of Bacillus amyloliquefaciensα-amylase induced by guanidine hydrochloride or urea.

ANS外源荧光探针结果表明：盐酸胍诱导的芽孢杆菌α-淀粉酶分子去折叠过程中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；而随着芽孢杆菌α-淀粉酶分子在盐酸胍溶液中变性程度的加深，这一疏水区域逐步被瓦解。丙烯酰胺和碘化钾猝灭结果表明：在盐酸胍溶液中，随着芽孢杆菌α-淀粉酶分子变性程度的进一步加深，其分子内能够被丙烯酰胺接近的色氨酸残基逐渐增多，直至全部被猝灭。但位于芽孢杆菌α-淀粉酶分子表面的能够被碘化钾猝灭的色氨酸残基，在中间态芽孢杆菌α-淀粉酶分子中数目达到最大的8个，而随着其分子变性程度的进一步加深，反而减少至5个。

Comma Splices 逗号连句 A comma splice is the use of a comma between two independent clauses.

逗号是不可以连接两个独立的单句的。

According to their individual shapes, they are Gram positive, catenulate rod-shaped bacteria that can produce elliptical endospore in the middle position. These characteristics are similar to Bacillus cereus' described in Bergey's Manual of Systematic Bacteriology. And the colony States, physiological and biochemical characteristics of the two strains also accord with Bacillus cereus', which suggests that they might be identified as Bacillus cereus.

通过菌株的个体形态观察，发现这两株菌是可以在细胞中间产生不膨胀的椭圆形芽孢的革兰氏阳性杆菌，且相互成链，这与伯杰氏手册中对蜡状芽孢杆菌的描述相似，另外对这两株菌的培养特征、生理生化特征的研究，也表明这两株菌符合蜡状芽孢杆菌的特性，故初步鉴定这两株菌为蜡状芽孢杆菌。

Consider to make clear in recent years, second acid is calcic - Bowman does not move bacillus clinical bacterium of single born of the same parents of the detached rate that go up and verdigris holiday comparatives, have the tendency that year after year rises, the fundamental condition pathogenic bacteria that already became the infection inside the courtyard [1] , have to a variety of antibacterial content be able to bear or endure higher property of a medicine [1, 2] , for this, we are monitored and statistic analysed my courtyard to came 1998 2004 7 years a second acid is calcic - the clinical infection condition that Bowman does not change bacillus, to 14 kinds of antibacterial content be able to bear or endure property of a medicine and be able to bear or endure medical tendency, sum up a report to be as follows now. 1 material and method all data all come from 1.1 basic materials to came on January 1, 1998 my courtyard outpatient service was mixed on December 31, 2004 the specimen sending check of inpatient, via clinical microbiology the lab is fostered and classics appraisal is second acerbity calcium - Bowman does not move bacillus to affect case of illness, with clinical and commonly used antibacterial at the same time content makes the kill that medical quick detects. 1.2 bacteria appraisal is mixed medical quick experiment uses live thing VITEK32 of Mei Li dust is full automatic the bacterial appraisal of bacterial analysis system and its form a complete set gets stuck and medical quick card undertakes detecting, 1998~2000 year use GNI appraisal gets stuck, 2001~2004 uses GNI+ card.

近年探究表明，乙酸钙-鲍曼不动杆菌临床上的分离率和铜绿假单胞菌相当，并有逐年上升的趋向，已成为院内感染的重要条件致病菌[1]，对多种抗菌药物有较高耐药性[1，2]，为此，我们监测并统计分析了我院1998年至2004年七年间乙酸钙-鲍曼不动杆菌的临床感染情况，对14种抗菌药物的耐药性及耐药趋向，现总结报道如下。1材料和方法1.1基本资料所有资料均来自于1998年1月1日至2004年12月31日我院门诊和住院病人的送检标本，经临床微生物学实验室培养并经鉴定为乙酸钙-鲍曼不动杆菌感染病例，同时以临床常用抗菌药物作药敏检测的结果。1.2细菌鉴定和药敏试验采用生物梅里埃VITEK32全自动细菌分析系统及其配套的细菌鉴定卡和药敏卡进行检测，1998~2000年使用GNI鉴定卡，2001~2004使用GNI+卡。

It's rather showy.I'd like something plainer.

赶花了，我想要素净些的。

The Kremlins Spassky Tower and passers-by are reflected in the wet cobblestones of Moscows Red Square on Feb.

克里姆林宫的斯巴斯克塔和旅客的身影倒映在潮湿的鹅卵石墙上。