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Expression of Caspase-3 and bcl-2 protein In E20 cerebral cortex, the expression of cleaved Caspase-3 in the encephalocoele group are higher than that in control group, and the expression of bcl-2 in the encephalocoele group are lower than that in control group. These findings were consistent with the results of the western blot analysis in E14 Brain.

四、Western-blot检测E14、E20胎鼠大脑皮质中caspase-3、bcl-2蛋白的表达 1、用Western-blot方法检测不同组E20胎鼠大脑皮质中caspase-3、bcl-2蛋白表达水平，结果表明对照组、给药无畸形组、脊柱裂组胎鼠大脑皮质中caspase-3、bcl-2蛋白表达水平无明显改变，而脑膨出组胎鼠大脑皮质中caspase-3蛋白活化片段表达水平明显增加，bcl-2蛋白表达水平明显减少。

Thick stem of the potato, in the Man-type, leaf green, kidney-type, petiole, stem are green, veins, geranyl Department of purple, purple-red skin potato, spindle type, the high rate of potato, sweet potato spring block 2-4 summer potato blocks 3-8, blockenlarged, smooth non-cleaved ditch resistance, storage, germination strong resistancelean, cooked noodles and sweet chestnut like incense and rich nutrition, high resistance to stem nematode, anti - root rot, is suitable for processing of starch, dry and cut the best varieties for human consumption.

该薯茎蔓粗壮，中蔓型，叶绿色，肾型，叶柄，茎蔓均为绿色，叶脉、叶基部紫色，薯表皮紫红色，纺缍型，大薯率高，春薯2-4块，夏薯3-8块，薯块膨大块，光滑无裂沟，耐贮藏，萌芽性强，耐肥耐瘠，熟食面甜而香似板栗，营养丰富，高抗茎线虫病、中抗根腐病，是适合加工淀粉、切干和食用最理想的品种。

Then the fusion protein was primary purified by affinity chromatography. The GST tail of the fusion protein was cleaved by Thrombin. Nucleotide sequence analysis showed that the gdh gene of S.suis possesses the highly conserved motifs typical of family I hexameric GDHs.

猪链球菌2型谷氨酸脱氢酶编码基因克隆、序列分析及原核表达根据已发表的猪链球菌2型的gdh基因设计引物，扩增海安病人分离株Habb中的目的基因，并进行序列测定和分析；构建重组表达载体pGEX4T-2-gdh，在大肠杆菌中表达，并纯化重组蛋白。

The conjugated structure of AOⅡ was reductively cleaved by micro-electrolysis, producing easily bio-degradable aromatic amines. The absorbance bands in the visible and ultraviolet regions were reduced or completely eliminated.

AOII分子在转鼓式内电解的作用下，其可见光和紫外光区的特征吸收峰逐渐降低直至消失，并新生成更容易生物降解的对氨基苯磺酸盐。

Second, residues in the reactive site of the inhibitor were replaced by the consensus substrate recognition sequence of furin, namely, Arg at P_1, Lys at P_2, Arg at P_4 and Arg at P_6. In addition, the P_7 residue Asp was substituted with Ala to avoid possible electrostatic interference with furin inhibition. Finally, the extra N and C terminal residues beyond the doubly conjugated disulfide loops were further truncated. However, all resultant synthetic peptides were found to be temporary inhibitors of furin and kexin during a prolonged incubation, with the scissile peptide bond between P1 and P'1 cleaved to different extents by the enzymes.

高活性、低分子量的furin酶抑制剂有可能发展成为新型的抗细菌内毒素和抗病毒感染的药物，我们以绿豆胰蛋白酶抑制剂Lys片段长链22肽为模板，设计了一系列能抑制furin和kexin活性的突变体，采用如下三种方法逐步优化：一、去除Lys片段长链三对二硫键中的一对，用Ser替换Cys，减少可能存在的二硫键错误配对；二、抑制剂的活性中心根据furin底物的专一性逐步改变，即P1、P2、P4和P6位分别变为Arg、Lys、Arg和Arg，同时为了避免P7 Asp和P6 Arg之间可能存在的相互作用，将P7位Asp替换为Ala；三、将两对二硫键形成的双环的外面5个氨基酸残基去除。

For long has this been cleaved to thus: this is mine, this is I, this is myself.

长久以来都在执著这些：这是我的，这是我，这是我自己。

That stubborn man always cleaved to his own ideas.

那个固执的人总不愿改变自己的观点。

"From the voice of my groaning my bone has cleaved to my flesh."

我的呻吟声听来，我的骨头已撕裂肌肉。

Compounds such as methane can be cleaved to carbon and hydrogen.

象甲烷这样的化合物能分解为碳和氢。

He cleaved an apple with a knife.

他用一把刀子将苹果切开。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。