查询词典 circulating library

New laccase gene and its full-length cDNA were clone from genomic library and equalized cDNA library of Ganoderma lucidum. Similarity between the sequence and other laccase gene were analyszed with NCBI blast, the results show that the new gene belong to multicopper oxidases gene family and have 60-70% Similarity with other laccase gene.

从灵芝的基因组文库和cDNA均一化文库中克隆了灵芝的漆酶基因和全长cDNA，并通过NCBI Conserved Domain Search氨基酸序列的相似性分析及与其它真菌漆酶之间的相似性分析，证明得到的蛋白是一种新的multicopper oxidases，其蛋白质序列与其它已克隆漆酶基因的同源性在6 0～7 0％之间，并对论文克隆的漆酶基因的启动子和终止子进行了分析。

In what District of Columbia Public Library Chief Librarian Ginnie Cooper calls an effort to make DCPL more secure and welcoming, the library has proposed banning sleeping in its facilities and a limit of two bags per person.

在哥伦比亚特区的公共图书馆馆长吉利库珀要求努力使图书馆更安全和更欢迎，图书馆已提议禁止睡在其设施内并限制每人只能带两个袋子。

Mr. Thoreau explained to the President that the railroad had destroyed the old scale of distances,--that the library was useless, yes, and President and College useless, on the terms of his rules,--that the one benefit he owed to the College was its library,--that, at this moment, not only his want of books was imperative, but he wanted a large number of books, and assured him that he, Thoreau, and not the librarian, was the proper custodian of these.

梭罗向馆长解释：现在火车已摧毁或扩大了借阅距离，----图书馆也就变得无用，是的，馆长和大学也就变得无用，在他自己看来，这所大学唯一对他的用处就是它的图书馆，----眼下，他不但是急用这些书，他还急用更大数量的书，而且梭罗向馆长保证，他，梭罗，而不是管理员是这些书的合法看管人。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

This paper points out that the library should establish the modern information knowledge service and resource guaranteeing system under the guidance of the scientific concept of development,and expounds the thought of people-based concept and reader first in the modern library.

阐述了现代图书馆&以人为本，读者第一&的思想，指出了图书馆应在科学发展观指导下，建立现代信息知识服务和资源保障体系。

Realizing the uncertainty legal statute of the "fair use" issue concerning digital library, this article moves to rethink the current copyright law and proposes to introduce the "implied license theory" and a digital library safe harbor into the copyright law.

在认识到Google数字图书馆在法律上是否构成合理使用的不确定性和模糊性之后，试图从理论上对著作权法进行反思，并提出在著作权法中引入&默示许可制度&和&数字图书馆'安全港'条款&的构想。

And the same time, with the development of academic education of our country, the academic library is transfer from single traditional library to the organization with comprehensive multi-functional, includes to provide the information of different fields and consultation serve, and the place with study, scientific research and associate.

同时，随着我国高等教育制度将向建立多元自主的特色方向前进，大学图书馆由单一功能的传统图书馆走向综合性多功能的信息服务中心，成为为读者提供各方面的情报、咨询服务的机构和学习、科研、交往的场所。

Centering on the demand of middle and primary school, the author develops the systems of library and digital library.

图书馆是学校文献情报中心，是为教学和科研服务的学术性机构。

During 1920s till 1930s, the famous library scientist Mr. Xiaoyuan Li advocated the spirit of popular library, which aims at protecting the same free reading rights of the lower class public as the upper class. To commemorate the 110th anniversary of Mr.

20世纪二三十年代，著名图书馆学家李小缘先生积极倡导民众图书馆精神，旨在保证处于社会底层的广大民众与强势群体一样享有同等的免费阅读的权利。

However, this result is possible only when there is recognition that library skills or information literacy is in collaboration with the library media specialist and the classroom teacher, supported by school principal and administrators.

然而这种结果只有在认知到图书馆资讯素养课程是图书教师和级任教师的合作，以及在学校行政人员的支持下，才有可能发生。

The system can be widely used in belt weighing and batching process control,etc.

介绍了基于智能模糊控制的嵌入式配料系统的设计方法，对模糊PID控制过程和系统调零作了重点的讨论，同时给出了PID控制的程序流程图和系统定长调零子程序。

"I dunno," she answered,"but if you show me aroun', I'll recognize it from the lighthouse just outside the porthole."

"我不知道，"她回答说，"如果你能带我转转，我能认出来的，因为灯塔就在舷窗外。"