查询词典 buffer bar

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

This invention discloses a multi-queue sequence buffer management circuit and a method based on a pipeline applying a pipeline structure including: an arbitration circuit selecting one for process from read, write and distribution buffer requests, a buffer slot state module designing state of the slot requiring operation and queue numbers and assigning idle slots, a buffer slot filter module filtering the slot, a buffer slot filter module filtering the slot states not belonging to the current operation queues nor idle aligned in terms of the head pointer, a queue slot selection module computing continuous idle slot numbers from the slot pointed by the head pointer and refreshing the head pointer and selecting preparing slots, a queue slot prior queuing module refreshing the read pointer and result numbers of the current operation queues with the pointer of the first prepare slots and their numbers which can support multi-queue to share one buffer space, queues can access the buffer in overlap.

本发明公开一种基于流水线的多队列顺序化缓冲管理电路及方法，本发明电路采用流水线结构，包括：仲裁电路，从读、写、分配缓冲请求中选取一路进行处理；缓冲槽口状态设置模块，设置请求操作的槽口状态和队列号，分配空闲槽口；缓冲槽口滤除模块，滤除不属于当前操作队列且非空闲态的槽口状态，按头指针对齐；队列槽口选择模块，计算头指针指向槽口起的连续空闲槽口数并更新头指针，选出预备态的槽口；队列槽口优先排队模块，用第一个预备态槽口的指针和预备态槽口数分别更新当前操作队列的读指针和结果数；本发明可以支持多个队列共享一个缓冲空间，各类指令队列能对缓冲进行交叉访问，并对指令结果的写入读出进行顺序化管理。

Through a combination of T7 RNA polymerase and T7 Promoter for testing proper buffer was selected.The experimental results showed 5×binding-Buffer 1 was more suitable for the test;TBE running-buffer was better than TAE and TGE buffer;0.2×TBE buffer was the best concentration amang there electrophoresis buffer.

通过用T7RNA聚合酶与T7启动子DNA的结合反应来选择合适的缓冲液，结果表明，不同的缓冲液会影响凝胶迁移试验的结果，5×Buffer1结合缓冲液的效果较好；TBE电泳缓冲液比TAE、TGE的电泳效果好，同时在3种不同浓度的TBE缓冲液中，0.2×的TBE效果最好。

Trans-Blot Cells and Systems 170-3825 Trans-Blot Cell With Wire Electrodes and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3850 Trans-Blot System With Plate Electrodes and PowerPac HC Power Supply, 100120/220240 V 170-3853 Trans-Blot System With Plate Electrodes, Super Cooling Coil, and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3910 Trans-Blot Cell With Wire Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3939 Trans-Blot Cell With Plate Electrodes and Super Cooling Coil, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3946 Trans-Blot Cell With Plate Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) Trans-Blot Cell Accessories 170-3912 Super Cooling Coil, required for all high-intensity transfers 170-3913 Gel Holder Cassette, includes 2 fiber pads 170-3914 Fiber Pads, 15.5 x 20.5 cm, 6 170-3920 Trans-Blot Standard Wire Electrode Card, cathode 170-3921 Trans-Blot Standard Wire Electrode Card, anode 170-3922 Trans-Blot Cell Buffer Tank 170-3923 Trans-Blot Cell Lid With Power Cables 170-3943 Trans-Blot Platinum Anode Plate Electrode 170-3944 Trans-Blot Stainless-Steel Cathode Plate Electrode 170-3945 Trans-Blot Plate Electrode Pair, platinum anode and stainless-steel cathode 16 规格:说明: Trans-Blot Plus

电泳转印槽组件 1。缓冲液槽及带有电缆的盖 2。凝胶支架转印夹 3。纤维衬垫 4。电极丝 5。电极板 6。特级冷却芯 Trans-Blot 转印槽是功能灵活的转印设备，可理想地用于多种转印应用。Trans-Blot 转印槽特点包括：*能进行多胶转印，可容纳3 块PROTEAN I xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶*多组参数灵活可设，可调节的电压设置（从30 V 的过夜转印到200 V 的1 小时快速实验）*电极间距设置为8 cm 用于标准印迹杂交，或设置为4cm 用于高强度印迹杂交*可选择板式电极：涂有铂金的钛作为正极，不锈钢为负极，能提供高强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝*通过特级冷却芯和水循环仪来调节温度―是天然酶（4°C）或高强度转印的理想选择，随着转印时间增加（多达24 小时），不会引起缓冲液耗竭（在高强度转印中必须使用冷却芯，也推荐用于所有板式电极的应用）*带铰链的凝胶支架转印夹能避免滑动，确保凝胶与印迹膜间的紧密接触；每个转印夹都有颜色标记以保证在转印槽中的正确定位 Trans-Blot 转印槽的锁闭凝胶支架转印夹系统。转印夹（1）支撑凝胶（2）印迹膜（3）两侧有纤维衬垫和滤纸（4）确保凝胶三明治内的完全接触。凝胶夹垂直插入缓冲液槽中（5）。

A polymeric buffer bar is employed to replace the conventional metallic transmission bar in a split Hopkinson pressure bar to determine the spalling strength of a long concrete rod that is placed between the metallic incident bar and the polymer buffer bar.

提出了利用Hopkionson压杆测量混凝土层裂强度的新实验方法：用高聚物材料取代传统的金属材料透射杆，混凝土试件为细长杆，由于高聚物波阻抗比混凝土小，试件中压缩波在试件／吸收杆界面反射后形成拉伸波使试件产生层裂破坏，通过吸收杆上透射波形可以确定混凝土层裂强度。

A rotor structure for a rotating wing aircraft having substantially rigid rotor head means and rotor blades or wing means arranged in at least one pair, comprising blade angle bearing means (10, 12, 14, 16) for each rotor blade means, tension bar means (6,8) operatively interconnecting the rotor blade means of a pair which blade means are arranged diametrically opposite each other, said tension bar means having a high bending resistance, whereby transmission of bending moments from the rotor blades to the rotor head means is substantially prevented, each of said blade angle bearing means comprising a structure angularly movable in the wing flap direction and in the wing lead-lag direction, at least one of said blade angle bearing means of a pair comprising means permitting an axial motion of the respective tension bar means supported in said blade angle bearing means, and wherein said blade angle bearing means (10, 12, 14, 16) operatively support said tension bar means (6, 8) on said rotor head means (4) for pitch angle rotation of said tension bar means relative to said rotor head means (4), said rotor structure further comprising means (24) securing said rotor blade means (18) to said tension bar means (6, 8) in a manner rigid against rotation of the blade means about the longitudinal blade axis whereby the securing means prevent flapping, drag and lagging movements of the tension bar means, said tension bar means being constructed as torsion bars which are elastic relative to torsion about said longitudinal blade axis.

1转子结构为旋转翼飞机，有大幅刚性转子头部的手段和转子叶片或机翼的手段安排中至少有一对，组成的叶片角度轴承手段（ 10 ， 12 ， 14 ， 16 ）为每个转子叶片的手段，拉杆手段（ 6，8 ）手术互连转子叶片的手段，其中一对刀片的手段都是安排截然相反的对方说，拉杆手段，有一个高弯曲阻力，即传输弯矩从转子叶片向转子头的意思是，大幅防患于未然，每个叶片角度说，轴承的手段组成的一个结构angularly动产在机翼上皮瓣的方向和在机翼导致滞后的方向，至少有一个叶片角度说，轴承是指一对组成的手段，允许轴向的议案，分别紧张酒吧手段，支持在叶片角度说，轴承的手段，其中，叶片角度说，轴承的手段（ 10 ， 12 ， 14 ， 16 ）手术的支持表示拉杆的手段（ 6 ， 8 ）就表示，转子头部的手段（ 4 ）为俯仰角轮换说拉杆手段相对说，转子头部的手段（ 4 ）表示，转子结构进一步组成的手段，（ 24 ）说，确保转子叶片的手段（ 18 ）表示，拉杆手段（ 6 ， 8 ）在一个僵化的方式对旋转的叶片即是说，约纵向叶片轴，即确保手段防止扑，拖曳和落后变动的紧张局势，酒吧的手段，说拉杆手段，正在建造的，作为扭杆这是弹性相对扭转约说，纵向叶片轴。

This kind of bar codes can be divided into achromatous function ink business card printing and membership card making bar code and non-ferrous function ink business card printing and membership card to make a bar code, the former is commonly used fluorescent ink and thermochromic ink, cyclometalated ink special ink to cards and membership cards produced by the bar code, this invisible bar codes in the business card printing and membership card must be used in making specific illumination, bar code recognition must be enclosed in the sensitive light source, this kind of bar code originally adelomorphic, and on functional inks business card printing and membership cards produced by the bar code is commonly used variable ink to cards and membership cards.

这种条码可以分为无色功能油墨制卡和会员卡制作条码和有色功能油墨制卡和会员卡制作条码，对于前者一般是用荧光油墨，热致变色油墨，磷光油墨等特种油墨来制卡和会员卡制作的条形码，这种隐形条码在制卡和会员卡制作中必须用特定的光照，在条形码识别时必须用相应的敏感光源，这种条码原先是隐形的，而对有色功能油墨制卡和会员卡制作的条码一般是用变色油墨来制卡和会员卡制作的。

Zheng Tiaojie with the son, referring to double-bar, night bar, true color bar, Feifan bar, a windmill with lounge, night Weiyang, happy bar, Witters, mangroves, Jue Shihao bar, night Weiyang, won it, Asia Metres high, and cheer it, Rafi Bar, the keys, such as the Blue Bar at Fort over 30 different styles of the bars.

整条街拥有金公子、双指吧、夜景吧、本色吧、飞帆吧、风车伴酒廊、夜未央、开心吧、威特斯、红树林、绝世好吧、夜未央、赢吧、亚米高、喝采吧、拉菲吧、金锁匙、蓝堡吧等30多个风格各异的酒吧。

The invention discloses a LCD panel, colorful filter piece and making method, which comprises the following steps: providing a base; forming a buffer material layer on the base; forming patterned lightproof layer on the buffer material layer; adopting patterned lightproof layer as lid to pattern the buffer material layer to form a patterned buffer layer; forming a separating piece through patterned lightproof layer and patterned buffer layer; making the separating piece form multiple pixel areas on the base; forming a colorful filter layer in each pixel area.

本发明公开了一种液晶显示面板、彩色滤光片及其制作方法，其中彩色滤光片的制作方法包括下列步骤：首先，提供一基板，并在基板上形成一缓冲材料层。接着在缓冲材料层上形成一图案化遮光层。以图案化遮光层为罩幕来图案化缓冲材料层而形成一图案化缓冲层，使得该图案化遮光层与图案化缓冲层共同形成一隔离件，且隔离件在基板上形成多个像素区域。然后在每一像素区域内分别形成一彩色滤光层。

Based on some related concepts of shock disturbed system, weakening buffer operators with variable weights and strengthening ones with variable weights are established respectively. This subject defines buffer operator regulation degree which reflects effect strength to original sequence caused by buffer operator, and also studie the relationship between buffer operator regulation degree and variable weight. Consequently, the monotonicity nonvariable axim of buffer operator is put forward.

基于冲击扰动系统的相关概念，提出了变权缓冲算子的概念，分别构造了变权弱化缓冲算子和变权强化缓冲算子，定义了缓冲算子调节度来反映缓冲算子对原始序列的作用强度，并研究了缓冲算子调节度与可变权重之间的关系，在此基础上提出了缓冲算子的单调性不变公理。

Fostering the concept of multi-culture firmly and mastering the development of advanced culture correctly are conductive to resisting terrorism, separatism of nationalities and ultraism of religion, which is of great academic theoretical, immediate and profound historical si...

牢固树立多元文化的理念和正确把握先进文化的发展方向，有助于在世界范围内自觉地抵制恐怖主义，民族分裂主义和宗教极端主义。不仅具有重大的学术理论意义，而且也具有重大的现实意义和深远的历史意义。

But if anyone walks in the night, he stumbles, because the light is not in him.

11：10 若在黑夜行走，就必碰跌，因为那光不在他里面。