查询词典 auxin

Isolation and identification the auxin binding protein is one of the major works in the studying of auxin receptor.

分离、鉴定与生长素结合的蛋白是生长素受体研究中的一个重要方面。

And this is determined by the asymmetric localization of auxin efflux protein in plasma membrane of auxin transport cell.

目前已知的植物激素中只有生长素具有极性运输这一特征。

Changes of IAA oxidase activity showed a parallel time curve while phenolic contents and auxin protector levels showed an inverse trend.

IAA氧化酵素之活性变化也有相似之时间曲线，而酚类含量及auxin保护成份之水位显示相反之时间变化。

These results suggested that the stimulation effect of local nitrate on LR growth is dependent on auxin. And the auxin mainly comes from the basipetal transport from shoot to roots, but not from the seed or the basipetal transport of auxin from root tip to LR growth sites.

这些结果表明，局部供应硝酸盐对侧根生长的促进作用依赖于生长素，这些生长素主要来源于地上部生长素向侧根生长部位的直接供应，而不是来源于种子或由根尖向侧根发生部位的生长素向基运输。

In order to explore the mechanisms of auxin action and metabolism, as well as the changing characteristics of its relative enzymes, IAA content in strawberry fruits with different treatments was determined by HPLC. Content of IAA and the activity of IAO and POD in fruits treated by NPA or TIBA, the inhibitor of auxin transport, were analyzed to investigate the changing characteristics and possible source of auxin, as well as the relation among IAA, POD and IAO. Results were showed as the followings: there had a certain count of IAA in receptacles and achenes, and IAA content in achenes was higher than that in receptacles.

摘要为了探索草莓果实中生长素的代谢变化及其发挥作用的机理，利用HPLC定量测定了草莓果实发育过程中瘦果和花托中的吲哚乙酸，结合生长素运输抑制剂NPA和TIBA处理考察了草莓果实中IAA含量以及不同存在状态的生长素氧化酶和过氧化物酶活性变化，综合分析了草莓果实中IAA 的变化特性、可能来源及其与生长素代谢相关酶的关系。

The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12 000 independent transformant lines were acquired by the above method, from which two mutants related to plant defense response and development, asr1 (auxin and .salicylic acid responsive 1.) and dai1 (brought tolerant and obscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSK1015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asr1(auxin and salicylic acid responsive 1)和dai1(drought tolerant and abscisic acid insensitive 1)。

The main work of this dissertation is constructing Arabidopsis gain of function mutants pool, screening mutants related to plant defense response, cloning the genes and studying their functions. The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12000 independent transformant lines were acquired by the above method from which two mutants related to plant defense response and development, asr1 (auxin and salicylic acid responsive 1) and dai1 (drought tolerant and abscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSKI015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asrl(auxin and salicylic acid responsive l)和dail(dought tolerant and abscisic acidinsensitive l)。

Auxin response factor is an important transcription factor for auxin signal transduction pathway. In order to identify the function of cotton auxin response factor GhARF3, some bioinformatics methods were used to analyze GhARF3 gene sequence, including BLAST analysis, domain analysis, phylogenetic tree analysis, the physical and chemical properties analysis of protein, hydrophobicity analysis, disulfide bonds analysis, phosphorylation site analysis, secondary structure and tertiary structure prediction, and subcellular localization and protein functional classification analysis.

运用生物信息学方法对棉花GhARF3基因序列进行BLAST分析、结构域分析、进化树分析、编码蛋白质的理化性质分析、疏水性分析、二硫键分析、磷酸化位点分析、二级结构以及三级结构的预测、亚细胞定位和蛋白质功能分类预测等分析。

First, the significant of co-factor InsP6 and center water in the mechanism of auxins are explored by docking; second, the experiments that compared with the docking rusults of two situations involving fully rigid and selective flexible of active residue of the receptor TIR1 illuminate that selective flexibility docking by AutoDock4 reports more rational results, so that, AutoDock4 dockings are implemented with TIR1-Auxins; third, AutoDock4 docking by TIR1-Auxins-Aux/IAA illuminates that auxin as a molecular glue enhances the interaction between TIR1 receptor and Aux/IAA substrate by the weak interactions, such as hydrogen bond and hydrophobic interaction, furthermore, the weak interactions between receptor protein and ligands greatly influence on auxin activity of auxin ligands.

首先， 通过分子对接计算研究辅酶InsP6以及中心水分子在生长素反应中的重要作用；其次，比较受体大分子完全刚性以及活性残基部分柔性的两种情况下的分子对接结果，说明AutoDock4实现了受体分子活性残基的部分柔性而使对接结果更加合理，进而使用AutoDock4方法对TIR1-Auxins体系进行对接计算；最后，对TIR1-Auxins-Aux/IAA体系进行分子对接计算，结果表明，生长素配体分子作为&分子胶水&直接与受体大分子TIR1以及底物多肽Aux/IAA形成强的弱相互作用，如氢键作用、疏水相互作用，促进了受体TIR1与Aux/IAA底物之间的结合，进而说明氢键作用和疏水相互作用等弱相互作用对于生长素分子的活性具有很大的影响。

Researchers have known since the nineteen thirties that the actively growing tip of a plant releases a hormone called auxin.

从十九世纪三十年代，研究人员已经知道植物活跃的生长锥释放一种称之为auxin的荷尔蒙。

It is also known as one of the most poisonous naturally occurring substances.

它也被称为一个最自然发生的有毒物质。

The greatest stress is found at the location on the cross section where V is the largest.

最大应力出现在横截面上V为最大的地方。