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The structural of mechanism adopted double pipes feed-in with mixing machine, special weighbridge for reducing bond; The hardware circuit make use of the programmable amplifier AD8555 which amplify the weak sensor signal and bridge imbalance compensation and gain error, at the same time,32-bit microprocessor LPC2134 was used as the core of the hardware to deal with the messages and send order to the implement machine. Software design transmits materiel by stages and FIR filter was adopted to remove big errors and enhance precision.

机械结构使用双管螺旋喂料机加搅拌机构，特殊的称斗结构；硬件电路采用数字可编程放大器AD8555作为称重传感器微弱电信号的放大器，以便于补偿桥式传感器的失调和增益误差，同时采用32位单片微处理器LPC2134作为整个电路的处理核心，及时处理称重信息，并向执行机构发送命令；软件根据称重值采取分阶段喂料，并加入了FIR软件滤波，滤除了粗大误差信号，提高了称重精度。

To amplify transferrin-binding protein 2 (Tbp2) gene by PCR from Actinobacillus pleuropneumoniae, a pair of primers were designed according to sequence Z46774 of A.

参照猪传染性胸膜肺炎放线杆菌血清5型菌株序列Z46774设计1对特异性引物，用PCR方法扩增转铁结合蛋白2(Tbp2)基因，得到1条1624bp的片段，然后克隆到pMD18-T载体中，经测序比较，与参考序列的核苷酸同源性达99。

The total DNA was extracted from four different species of Chironomus and used as template to amplify the 18s rRNA ITS1 by PCR and then the 18s rRNA was analyszed by enzyme. The method and condition of molecular classification of Chironomus was summzrized.

尝试了对摇蚊分子分类的研究，通过对4个不同种类摇蚊的DNA提取、PCR扩增和酶切分析，总结出摇蚊分子分类的条件和方法。

Among three microsatellite primers usually used in the aphid and three primers used in the study of the Aedes japonicus (Diptera:Culicidae), finally four primers were selected to amplify the different samples of the green peach aphid. The result showed that DNA samples could be well amplified.

在三条常用的蚜虫微卫星引物和三条用于对日本伊蚊Aedes japonicus 研究所用的引物中，最终选取了33.15 核心序列、4、8G、8G 四条引物对桃蚜的不同样本进行扩增，结果表明扩增效果较好。

Long-distance PCR was used to amplify the β-actin gene for black carp based on the β-actin gene sequences of Cyprinidae. Construct of EGFP expression system containing 5'UTR of β-actin gene for black carp was transferred into zygotes of mud loach and COS-7 cells and the results showed that 5'UTR of β-actin gene for black carp could be used as the promoter of the"all-fish"transgene.

基于鲤科鱼类Beta-肌动蛋白基因序列，采用长片段PCR技术，克隆了青鱼β-actin基因DNA片段，并构建了青鱼β-actin基因5'启动调控区绿色荧光蛋白表达载体，通过显微注射泥鳅胚胎和COS-7细胞转染实验，证明了所克隆的青鱼β-actin基因5'启动调控区具有启动调控功能，并用之来构建&全鱼&基因的启动子部分。

In order to simplify the procedure and obtain high quality gDNA, we alternate water-bath to dewax, extract gDNA from hepatocellular carcinoma embedded by paraffin, and amplify gene using HCC gDNA.

为了简化操作，获得更高质量的gDNA，我们采用水浴法替代二甲苯进行脱蜡，亲和层析纯化石蜡包埋肝细胞癌组织标本中基因组DNA，以纯化gDNA为模板PCR扩增看家基因。

An epicyclic unit was also fitted between the coupling and rear differential to amplify speed differences between coupling input and output.

一个单位也是行星之间的耦合装置和后部之间的差距继续扩大耦合输入和输出速度的差异。

We amplify the method of test and research the data, then draw the conclusion of system application in the finished oil pipeline from Karamay to Urumchi, according to experimental effect and give the problem in exite and advise for improvement.

说明了调试方法，对测试数据进行了分析，根据试验效果对该系统在克-乌成品油管道上的适用性做出了结论，提出了存在问题和改进建议。

Objective To clone the flagellar biosynthesis genes fihA, flhB2 and fliR of Leptospira interrogans and construct their prokaryotic expression system Methods Extract genomic DNA from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai strain 56601 by phenol-chloroform method and amplify fihA, flhB2 and fliR gene fragments by high fidelity PCR.

目的 克隆问号钩端螺旋体鞭毛相关基因flhA、flhB2和fliR，并构建其原核表达载体。

Which will amplify greatly the resource of artworks elements and resolve forenamed problem at a certain extent. Color is also one of the most important ingredients, so we should also supply functions of color changing.

由于孟赛尔色彩协调理论很难用精确的规则来表示，我们引入了直观而且符合人的形象思维习惯的基于实例的推理技术。

Yet, Edith says, there was always money in the bank.

但是Edith说，她的银行户口里面总是有钱。

We have created a series of files that resolve this problem--one for each day of the 40 days of purpose.

我们制作一系列的档案去解决这问题---一个为标竿40天中的每一天。