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Changdong" showed a noticeable enhancive effect when it was co-administrated with other anti-tumor agents or when it was co-applied with radiotherapy. The action mechanism study showed that an apparent cytotoxic effect of "Changdong" on ten tumor strains in vitro with a quantity-effect relationship was observed. Immunity experiment showed that "Changdong" could induce proliferation of spleen lymphocyte in mice bearing tumor and could improve NK cell" s activity, whereas has no impact on IL-2" s reproduction. It was also noticed that "Changdong" could induce human liver cancer QGY" s apoptosis in vitro, and it happened mainly on G0-G1 phase.

结果:"长动"对小鼠移植瘤模型和人体肿瘤裸鼠异种移植均有明显抑制作用,量效关系明显:"长动"与放疗和化疗分别联合应用有明显的增效作用:作用机制研究结果:细胞毒性试验研究表明"长动"在体外对10株肿瘤细胞有明显的细胞毒性作用,量效关系明显;免疫学试验表明"长动"可以诱导荷瘤小鼠的脾淋巴细胞增殖,增强NK细胞的活性,对IL-2的产生无明显影响;"长动"在体外可明显诱导人体肝癌QGY细胞凋亡,使细胞阻滞于G0-G1期。

Methods Atherosclerotic model was developed by feeding the rats with high-cholesterol-fat diet for nine weeks and Equisetum extract and the rest of the extract were administrated to the rats at the same time.

选用雄性SD大鼠,复制食饵性动脉粥样硬化早期模型,用木贼提取物、提取剩余物分别预防性给药,以吉非罗齐为阳性药对照。

Adult male SD rats were randomly divided into five groups:control group、hypercholesterolemia control group、dosage group with 72.3%,80.5%,96.1% deacetylated flyblow chitosan.The rats were fed with a high cholesterol diet and were administrated flyblow chitosan continuously for 8 weeks at the same time,then the blood lipid and weight were measured.

取成年雄性SD大鼠随机分为5组,分别为正常对照组、高脂模型组、蝇蛆壳聚糖72.3%,80.5%,96.1%脱乙酰度组,给高脂饲料造模的同时连续给予蝇蛆壳聚糖8周,测定大鼠血清总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白含量,同时观察对大鼠体重影响。

Methods We made gastrostomy in rats first,and special amount of alcohol was administrated via gastrostoma by a tube everyday associated with highly fatty food.

先给大鼠置一个长期人工胃管,经胃造瘘管每日注入一定量酒精,辅以高脂肪饲料。

Experiment 3: Affections of Ginger Extraction on Cardiac Systolic/Diastolic Function of Rabbits with Heart Failure and the Comparison Between Ginger Extraction and 4-Retro-decoction (Si-hi Tang,四逆汤) Method 50 pure-breed New-Zealand rabbits were randomly allocated into 5 groups: control group, low (0.028mL/kg), middle (0.056mL/kg) and high (0.112mL/kg) dosage Ginger group, and 4-Retro-decoction group. The heart failure model of rabbit was established by intravenous administration of 20g/L pentobarbital sodium (0.15 mL·kg〓·min〓) at a certain rate. Oral tube perfusion of Ginger extraction was administrated after the establishment of the model. Their hemodynamic changes were detected before and after the model establishment, and at 5, 10, 15, 20, 30, 45, 60, 90, 120, 150 minutes respectively after the ginger administration by a RM-6000 4-graph physiological monitor.

干姜提取物对兔急性心衰模型心脏功能及血流动力学的影响及其与四逆汤作用的比较方法以50只纯种新西兰兔为研究对象,随机分为低、中、高剂量干姜组,溶剂对照组和四逆汤对照组,共5组,以0.15mL·kg〓·min〓静脉恒速给予20g/L戊巴妥钠的方法建立兔急性心力衰竭模型,造模成功后,经口插管入胃灌服受试品,用RM—6000四道生理仪测定造模前、造模成功时及用药后5min、10min、15min、20min、30min、45min、60min、90min、120min、150min兔的心肌舒缩性能指标,观察各时段指标的变化情况。

Methods 40pure-breed New-Zealand rabbits were randomly divided into4groups:low(0.028mL/kg),middle(0.056mL/kg),high(0.112mL/kg)dosage ginger group and4-Retro-decoction group.The heart failure model of rabbit was established by intravenous administration of20g/L pentobarbital sodium at a certain rate.Oral tube perfusion of ginger extract and4-Retro-decoction were administrated after the establishment of the model.Their hemodynamic changes were detected before and after the model establishment,and at5,10,15,20,30,45,60,90,120,150minutes respectively after the ginger administration by a RM-6004-graph physiological monitor.

以40只纯种新西兰兔为研究对象,随机分为高、中、低剂量干姜组和四逆汤组共4组,用恒速滴注戊巴比妥钠的方法建立兔急性心力衰竭模型,造模成功后,经口插管入胃灌服受试品,用RM-600四道生理仪测定造模前、造模成功时及用药后5min、10min、15min、20min、30min、45min、60min、90min、120min、150min兔的心肌舒缩性能指标,观察各时段指标的变化情况。

Objective To investigate the difference between the effects of Ginger Extract and4-Retro-deˉcoctionon cardiac systolic/diastolic function of rabbit with heart failure.Methods 40pure-breed New-Zealand rabbits were randomly divided into4groups:low(0.028mL/kg),middle(0.056mL/kg),high(0.112mL/kg)dosage ginger group and4-Retro-decoction group.The heart failure model of rabbit was established by intravenous administration of20g/L pentobarbital sodium at a certain rate.Oral tube perfusion of ginger extract and4-Retro-decoction were administrated after the establishment of the model.Their hemodynamic changes were detected before and after the model establishment,and at5,10,15,20,30,45,60,90,120,150minutes respectively after the ginger administration by a RM-6004-graph physiological monitor.

目的 探讨干姜提取物和四逆汤对心衰兔心脏舒缩性能的作用异同方法以40只纯新西兰兔研究对象,随机分为高、中、低剂量干姜组和四逆汤组共4组,用恒速滴注戊巴比妥钠的方法建立兔急性心力衰竭模型,造模成功后,经口插管入胃灌服受试品,用RM-600四道生理仪测定造模、造模成功时及用药后5min、10min、15min、20min、30min、45min、60min、90min、120min、150min兔的心肌舒缩性能指标,观察各时段指标的变化情况。

The first aim of this study is to clarify whether that polymorphisms of the genes that encode these enzymes associates with the TB drugs-induced adverse reactions. Furthermore, since tuberculosis patients are always administrated with multiple TB drugs, it is hard to distinguish which TB drug caused these adverse reactions.

本研究从结核药物过敏病患代谢酵素基因多型性方面探讨与结核药物过敏反应发生的相关性;此外,由於结核病患通常同时接受多种结核药物的治疗,导致难以判断是其中何种药物引起不良反应,本研究另一目标即为建立一T细胞体外培养系统,用於判断何种药物引起不良反应的产生。

RAFE at all doses administrated interruptedly by gastric tube above 13 w caused chronic renal tubulo-interstitium fibrosis.The renal injury in functions and tissue forms in rats were similar with AA closely.The results showed that AA was the main toxic composition of RAFE.

RAFE小、中、大剂量灌胃给药13周以上可致大鼠慢性肾小管-间质纤维化,其肾功能和肾组织形态表现上与马兜铃总酸所致肾损伤相似,结果提示马兜铃酸是RAFE主要毒性成分。

Seven days after inoculation, nude mice were intraperitoneally administrated with drugs.

实验干预:接种后7 d 开始腹腔注射给药。

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