查询词典 adm

The mechanism of GCT restrained by ADM: To determine the mechanism of GCT restrained by ADM, the GCT cells were cul- tured with ADM (the dense of ADM after adding were 0.04μg/ml, 0.4μg/ml and 4μg/ml) for 72 hours. The GCT cell were observed by microscope and electron lens, analyzed by flow cell counter and DNA electrophoresis.

阿霉素抑制骨巨细胞瘤的机理：为探讨阿霉素抑制骨巨细胞瘤的机理，我们用阿霉素与骨巨细胞瘤细胞悬液共同培养72小时（阿霉素最终浓度分别为0.04μg/ml，0.4μg/ml及4μg/ml），搜集细胞，用光镜、电镜观察及用流式细胞仪、DNA电泳进行分析。

As metal ions can interact with both of ADM and DNA, they undoubtedly affect the interaction between ADM and DNA. Addition of a large excess of Cu or Fe into a solution of well-formed ADM-DNA complex makes the metal ions simultaneously bind to ADM and DNA to form a ternary complex. Otherwise,〓 or 〓 complexes can bind directly to DNA to form a ternary complex.

金属离子-ADM-DNA三元体系的光谱研究表明，当ADM与DNA结合后，大量Cu或Fe的加入可以使金属离子进入到ADM-DNA复合物中，并同时与ADM和DNA键合，形成三元复合物；反之，若ADM先与Cu或Fe配位，生成的金属配合物则能够直接与DNA结合形成三元复合物。

Heat treatment can apparently confronte multidrug-resistant of 7721/Adm and increase the sensitivity of 7721/Adm to ADM.,and the resulet indicate that increasing the sensitivity can be related to intracellular concentration of ADM.

结论加热可以显著对抗7721/Adm的耐药性，提高其对阿霉素的敏感性，这与加热提高了细胞内药物浓度有关。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

By immunohistochemistry and semiquantitative RT-PCR analysis, we were the first to demonstrate that AdM mRNA and immunoreactive AdM exists in the rat brain distributed in cerebral cortex, paraventricular tissues, hypothalamus, mesencephalon, medulla oblongata and cerebellum, and AdM mRNA was also found in the human brain.

应用免疫组织化学和半定量RT-PCR方法，首次发现在大鼠的脑内，包括大脑皮层、室旁组织、下丘脑、中脑、延髓和小脑均有AdM mRNA及AdM的免疫活性物质存在；在人脑内也含有AdM mRNA。

Six weeks after drug intervention,we found,in ADM+Res group,the mean tumor volume of MCF-7/ADM cells implanted tumor was 1.203±0.338 cm~3 with statistical significance to compare with other groups(P＜0.05),decreased about 50.58%compared with the mean tumor volume of ADM alone group1.979±0.307 cm~3the result of the mean tumor weigh was alike to the mean tumor volume; compared with the control,Res could relieve weigh loss(P＜0.05,and the toxicity of treatment was not obvious;Microscopic examination showed that there was massive area of cytoclasis and apoptosis in the tumor tissue of the combination group comparing with other groups.

实验结果提示用药6W后，ADM+Res组移植瘤体积(1.203±0.338)cm~3明显小于其余各组，抑瘤率为50.58%，与单用ADM组(1.979±0.307)cm~3相比有差异P＜0.05肿瘤重量的结果与瘤体结果类似；未观察到用药后的毒副作用；经不同处理后，单独注射Res组小鼠体重较生理盐水组及ADM组有所增加(P＜0.05，而ADM+Res组治疗前后体重无差异P＞0.05HE结果显示与其他组相比，ADM+Res组肿瘤组织中出现大片坏死区域。

Hyperthermia can increase the sensitivity of 7721 and 7721/Adm to ADM.

加热可以显著提高人肝癌细胞-7721和耐药人肝癌细胞模型-7721/Adm对阿霉素的敏感性。

Taking untreated MCF-7/ADM cells as controls,flow cytometry was applied to detect the intracellular concentration of rhodamine123 in MCF-7/ADM cell intervened with lobeline of 20 μmol/L.

同时用流式细胞术检测洛贝林对MCF-7/ADM细胞内罗丹明123积聚浓度的影响，从功能学的角度观察洛贝林的耐药逆转作用及其机制。

In hypertensive patients with heart failure,the ratio of ADM/ADT decreased with the deterioration of heart failure Conclusion:The oversecretion of ADT and slow compensation of ADM is a characteristic deregulation of vasoactive peptide in the process of hypertensive heart failure.

ADM代偿缓慢和ADT分泌过度是高血压心力衰竭过程中血管活性肽调节紊乱的一个特点。

Methods:(1) ADM was produced from swine skins treated with trypsin followed by Triton X-100.(2) type I collagen of mouse tail was extracted by 0.5mol/L acetoacetic acid.(3) Two kinds of ADM were got by being soaked with 0.02% glutaraldehyde for 5~10 min or being soaked with mouse type I collagen for 24h, then preserved at 4℃.

(1)应用胰蛋白酶消化-去污剂法制备ADM；(2)用0.5mol/L乙酸溶液提取制备I型鼠尾胶原；(3)用0.02%戊二醛溶液浸泡ADM5-10min，制成交联型ADM；另一部分ADM用质量分数0.25%的I型鼠尾胶原浸泡24h，制成胶原包埋型ADM，冰冻保存；(4)用酶消化法培养原代成纤维细胞，取第三代增殖期细胞，将其调整至浓度为2×105/ml的成纤维细胞悬液。

Sodium citrate; calcium carbonate; calcium hydroxide; potassium bicorbonate; magnesium carbonate; ferrous sulphate; Zinc sulphate; copper sulphate; manganese sulphate; potassium iodide; sodium selenite.

柠檬酸钠；碳酸钙；氢氧化钙；碳酸氢钾；碳酸镁；硫酸亚铁；硫酸锌；硫酸铜；硫酸锰；碘化钾；亚硒酸钠。

All this to say that I'm glad filmmaking hasn't become completely ironic. I can enjoy an ironic film as much as the next person, but they usually involve a message of some sort. One that's delivered in a smirkingly clever way that requires little in the way of talent.

所有这些都是说，我很高兴电影制作没有变成完全讽刺艺术，我可以和下一个人一样享受讽刺电影的乐趣，虽然他们经常会被划归到一定级别，技术方面不需要很高的才能。