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However, the metals showed their neurotoxcity, more by enhancing the activity of AChE in copepod. Nevertheless, after 12 d exposure of Cd or Ni, at more extent, MDA level in copepods are little correlated with metal treatments.

同时,镉和镍对桡足类均有神经毒性效应,因为不同浓度重金属均诱导桡足类体内AChE活性的显著变化,但重金属更多是通过AChE活性的提高而呈神经毒性。

Accordingly, the activities of AchE and CarE on the larvae of diamondback moth raised on 5 C are much more than that of raised within the range of 10C to 30 C.

相应地,在5℃的低温条件饲养下小菜蛾的AchE和CarE比活力均大大高于在10℃一30℃范围内饲养的AchE和CarE比活力。

Through the methods of biochemistry, detecting the active of AchE and CarE and thecontent of protein in the eggs of Bactrocera dorsalis in the time of

随着发育进行Pr含量逐渐降低;AchE酶活是先增强,随后降低,随后又增强,其中在48h时AchE酶活最低,而CarE酶活在整个发育过程中都没有表现。

In addition, the maximum velocity in larvae was significant higher than those in pupae and adults. In vitro inhibition studies of larvae AChE indicated that the I50 values of 20-d larvae were higher than those of 10-d larvae when treated with eserine, malaoxon and carbaryl, respectively, suggesting that AChE in 20-d larvae was less sensitive to the inhibitors.

通过对幼虫AChE离体抑制作用测定表明,毒扁豆碱、马拉氧磷和西维因对20d龄幼虫的抑制中浓度I50均高于10d龄幼虫,说明20d龄幼虫体内的AChE对3种抑制剂的抑制作用更不敏感。

Its hypotoxicity and high selectivity against AChE make become the ideal leading compound in developing new AChE inhibitors.

土震素B具有低毒性、高选择性等优点,是发展新的AChE抑制剂理想的先导化合物。

The 6 AChE Genes from L.bostrychophila,L.entomophila and L.decolor were demarcated into Insect TypeⅠAChE Gene and Insect TypeⅡAChE Gene.4 Gene cloning of nAChR and mRNA expression level in L.bostrychophilaTwo nicotinic acetylcholine receptor subunit genes,Lb al and Lb a8,were cloned from the psocid L.bostrychophila.The full length cDNAs of Lb a1(GenBank Accession No.: EU871527) and Lb a8GenBank Accession No.

昆虫的两类AChE严格的区分,其中Ⅰ型AChE先与脊椎动物AChE聚合后才与Ⅱ型AChE相聚,说明两类AChE基因在物种分化前就已经分化。4嗜卷书虱烟碱型乙酰胆碱受体基因克隆及其mRNA表达水平研究利用RT-PCR和RACE技术成功克隆获得了嗜卷书虱2个烟碱型乙酰胆碱受体亚基的全长序列,分别命名为Lb a1(GenBank登录号:EU871527)和Lb a8(GenBank登录号:EU871526)。

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具,分别以人丁酰胆碱酯酶(1p0i:A)和果蝇乙酰胆碱酯酶(1d×4:A)的蛋白晶体结构为模板,对嗜虫书虱2个AChE的三维结构进行同源建模,并在三维结构中发现了AChE的酶解活性位点,证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少,在GenBank中没有可用作内参基因的序列,因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号:FJ041117),该片段长度为822 bp,编码273个氨基酸残基,同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

In contrast,the expression levels of Lb est2 in nymphal stages are lower than that in adult stage.2 Gene cloning of AChE and mRNA expression level in L.entomophila2.1 Gene cloning and sequence analysis of AChE in L.entomophilaTwo full length cDNA encoding AChE were cloned from L.entomophila by the methods of RT-PCR and RACE,named Le ace1(GenBank Accession No.:EU854149) and Le ace2GenBank Accession No.

CarE基因在不同发育阶段mRNA表达量的分析结果表明,Lb est1的表达量随着该虫生长发育的进行逐渐降低,至成虫期时表达水平最低;相反,L6 est2在若虫期表达水平较低,而在成虫期表达水平最高。2嗜虫书虱乙酰胆碱酯酶基因克隆及其mRNA表达水平研究2.1嗜虫书虱乙酰胆碱酯酶基因克隆及序列分析利用RT-PCR和RACE技术成功克隆获得嗜虫书虱2个AChE基在的全长序列,分别命名为Le ace1(GenBank登录号:EU854149)和Le ace2(GenBank登录号:EU854150)。

Activity of s AChE and GSTs was elevated in 4 strains,of which the activity of AChE and GSTs in KFQ strain and BYS strain was 1.57 fold and 2.33 fold respectively compared with susceptible strain.Activity of AChE and GSTs was decreased in one strain,activity of AChE was elevated but activity of GSTs was decreased in other 3 strains.

有4种品系可使AChE和GSTs活性提高,其中KFQ品系、LD品系与敏感品系相比,AChE的活性分别提高了1.57倍和1.44倍;BYS品系使GSTs活性提高了2.33倍;有3种品系可使AChE活性提高,使GSTs活性下降;另有一种品系使AChE和GSTs活性均下降。

Results:Two different types of cells were observed in rat myenteric plexus:AChE-positive neurons,most of them being strong AChE activity,were circular or ovala few were triangle or ir...

结果:光镜下,在回肠肌间神经丛内,可观察到两种不同染色的细胞:① AChE 阳性神经元,大多呈 AChE 强阳性,胞体多为圆形或卵圆形,部分为三角形或不规则形,体积大,胞体直径达20~25μm,胞浆内含有棕褐色 AChE 阳性颗粒,细胞核不着色。

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