- 更多网络例句与筛选法相关的网络例句 [注:此内容来源于网络,仅供参考]
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One cDNA clone was identified by immunoscreening from a cDNA expression library prepared from Babesia gibsoni merozoite mRNA and its complete nucleotide sequence was 2108bp in length,including one single open reading frame and encoding a 62ku protein which had high homology with that of apical membrane antigen-1(AMA-1) of other species.
通过免疫筛选法,从构建的犬吉布森巴贝虫cDNA基因表达文库中筛选到1个cDNA克隆,此cDNA核苷酸全长2108bp,包含1个完整的开放阅读框,编码蛋白的分子质量约为62ku。
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After 4 passages in culture, the MSCs were implanted into 96 foveola with cell density of 0.08,0.1,0.2,0.4,0.6,0.8,1,2,4,6,8,10(x104/ml) respectively. Meanwhile, the MSCs were cultured in Dulbecco's modified Eagle's medium culture medium with different concentration of fetal bovine serum, the 3-4,5dimethylthiazol-2-yl-2,5-diphenyl tetrazelium bromide assay were used to determine the proliferation index of MSCs 72 hours later, the cell cycle were determine by cytometer also.
贴壁筛选法分离、纯化MSCs,取4代以后细胞,以不同密度接种于96孔培养板内,分别以不同浓度血清进行培养,72h后,采用四甲基偶氮唑盐比色法测定MSCs增殖能力并用流式细胞仪测定细胞周期。
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Screening anti-diabetes and anti-hyper activity of MCP with high throughput screening was studied in this paper.
利用高通量筛选法对苦瓜多糖的分离纯化产物进行降血糖降血脂活性研究。
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Cloning and characterization of novel genes involved in cell growth and prolifereation using the strategy of homology screening;;Cloning,chromosome mapping and tissues expression patterns of NQO3A2 and human hereditary methemoglobinemia gene
中文题名应用同源筛选法分离与克隆细胞生长、增殖相关基因的研究;人类NQO3A2 cDNA的克隆、定位,及其与NQO3A1表达谱的比较分析
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RESULTS: BMSCs was pure following density gradient centrifugation and adherence screening method. The third and fifth passage of cells had typical whirlpool-shape. Transmission electron microscope demonstrated that round or oval MSCs possessed large nuclei, big nucleus proportion, a few cellular organ.
结果:①经密度梯度离心法、贴壁筛选法并结合传代方法所得的间充质干细胞很纯,第3代和第5代细胞形态单一,细胞排列具有典型的漩涡状结构。
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Present methods for diluting were briefly introduced, and a new method, selection from point to point, was put forward and demonstrated to be a practical method. In the end,several problems about right-angle processing,deleting redundant points and lines were s imply discussed.
概述了目前已有的几种曲线点抽稀方法,提出了一种新的方法——逐点筛选法,并且用实例证明了它的实用性;最后,简要介绍了进行直角化处理、重复点线的删除以及接边时要注意的问题。
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A moderately halophilic bacterium strain DF-B6 capable of degrading Tween 20 was isolated from solar saltern by plate method.
利用平板筛选法从盐场卤水池中筛选到一株高效降解Tween 20的中度嗜盐菌DF-B6菌株。
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Use tertiary system method to screen and optimize JLNCAO substrate, and use heat and dry under atmospheric pressure method, ignition residue method, The Casparian law of trace nitrogen, acid...
运用三级系统筛选法对菌草培养基进行筛选优化;用常压加热干燥法、灼烧法、微量凯氏定氮法、酸性洗涤剂法、氨基酸自动分析仪、原子荧光光谱和原子吸收光谱、乙酰丙酮法和热水提取多糖法分别对菌草香菇和木屑香菇的水分、灰分、蛋白质、纤维素、氨基酸、重金属、甲醛、香菇水溶性多糖等的含量进行比较研究。
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This paper reviewed detailedly chemical structure,physical and chemical characteristics,relation of structure and effect,toxicity of chloramphenicol,metabolism and residual in animal body,measurement method of residual of chlor...
本文就氯霉素的化学结构、理化特性、构效关系、毒性作用,在动物体内的代谢与残留,检测方法中的筛选法、定量测定法、确证法等进行了详尽阐述。
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Six new Bt strains, W14, ZY1, 3346, TMQ2, B31 and P91, were isolated from different habitats by NaAc-antibiotics method. Small- rhomboidal crystals were observed under microscope.
通过醋酸钠-抗生素筛选法从6个不同生境中筛选得到了苏云金芽孢杆菌新菌株W14、ZY1、3346、TMQ2、B31和P91,镜检可观察到它们的伴孢晶体呈小菱形和不规则形状。
- 更多网络解释与筛选法相关的网络解释 [注:此内容来源于网络,仅供参考]
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Western blot:法
检测TIP30蛋白在涎腺腺样囊性癌高转移细胞中的表达,检测携带外源性TIP30细胞的细胞周期分布变化.方法应用脂质体转染方法将TIP30导入ACC-M细胞中,G418筛选阳性克隆,用免疫印记杂交法(Western blot)检测转染细胞中TIP30蛋白的表达;
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group factor:组因子
可分组旋转设计 group divisible rotatable designs | 组因子 group factor | 群筛选法 group screening methods
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sieve method:筛选法,逐步淘汰法
sield-stop reticule combination 视场光阑-调制盘组合 | sieve method 筛选法,逐步淘汰法 | sift converter 移相变换器
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sieve method:筛选法
筛目 sieve mesh | 筛选法 sieve method | 筛目孔径 sieve size
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sieve analysis method:筛选法
sierra 山脉 | sieve analysis method 筛选法 | sieve analysis 筛选
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Major Ampullate Gland:主壶腹腺
文章摘要:首次通过反转录-置换法和使用pUC18质粒成功构建大腹圆蛛(Araneus ventricosus)主壶腹腺(major ampullate gland)cDNA基因文库,并以鸟枪法从中筛选出具有典型重复结构的大腹圆蛛主壶腹丝蛋白cDNA基因AvF1,大小为1744bp,编码区为1572bp,
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Trimeresurus stejnegeri:竹叶青
利用逆转录酶与聚合酶链反应相结合的RT-PCR法,扩增出5个竹叶青(Trimeresurus stejnegeri)蛇毒丝氨酸蛋白酶的cDNAs;将扩增的cDNA片段克隆入pGEM-T载体中,筛选得到它们的基因,分别命名为TSSP-1、TSSP-2、TSSP-3、TSSP-4和TSSP-5.经末端终止法测定核苷酸序列,
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path filtrating method:路由筛选机制
有序搜索法:Predictor based Ordered Search Method | 路由筛选机制:path filtrating method | 关键线路法:Critical Path Method
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blue-white selection:蓝白斑筛选
blotting 印迹法 | blue-white selection 蓝白斑筛选 | blunt end 平(整末)端
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blue-white selection:<基因词汇Gene> 蓝白斑筛选
blotting <基因词汇Gene> 印迹法 | blue-white selection <基因词汇Gene> 蓝白斑筛选 | blunt end <基因词汇Gene> 平(整末)端